毛细管区带电泳法分析不同来源血竭中龙血素A和龙血素B

在系统优化了电解质溶液的pH、组成、浓度及仪器条件的基础上,建立了一种测定不同来源血竭中龙血素A和龙血素B的毛细管区带电泳(CZE)方法。采用20 kV的分离电压,25 ℃的毛细管柱温,211 nm的检测波长以及5 s的压力(3447 Pa)进样时间,在20 mmol/L的Na2B4O7缓冲溶液(用NaOH调节pH到9.98,含有10%(v/v,下同)乙腈、5.0%乙二醇和1.0%正丁醇)中,龙血素A和龙血素B在15 min内得到了有效分离与检测。方法的线性范围对于龙血素A和龙血素B分别为1.0～100.0 mg/L和0.5～100.0 mg/L。将该方法用于天然血竭及人工诱导血竭中龙血素A和龙血素B的测定,相对标准偏差在0.6%～3.8%之间,加标回收率在95.1%～105.8%之间。方法具有简单、快速、重现性较好和准确度较高的优点,可以用于血竭样品中龙血素A和龙血素B的测定。     

一种新型的化学离子敏（1）一次性血液电解质钾、钠测试卡片的研制和应用

新型的一次性化学离子敏钾、钠测试卡片，是基于离子选择电极（ＩＳＥ）原理设计制作的一种电化学传感器~［１］。它由测量电池装置、敏感膜、接触衬底（石墨）和盐桥等构件组装而成。采用两点电位测量法。具有一次性使用、简便、快速、精确度高、成本低等优点，对样品不必作预处理，避免了交叉污染，克服了ＩＳＥ重现性差、固有漂移等缺点，发挥了ＩＳＥ选择性好、准确度高等优点。根据临床应用和抽样测试结果，钾、钠测试卡片的精密度±ＳＤ：钾１．０；钠２．０，Ｃ．Ｖ％：钾３．０％；钠２．０％；响应时间为３０ｓ；漂移：钾０．３ｍＶ／ｍｉｎ；钠０．５ｍＶ／ｍｉｎ；回收率为９９．５％，均符合临床应用的基本要求。     

Performance of a sound card as data acquisition system and a lock-in emulated by software in capillary electrophoresis

The performance of fluorescence detectors in capillary electrophoresis is maximized when the excitation light intensity is modulated in time with optimal frequencies. This is especially true when photomultiplier tubes are used to detect the fluorescent light. The photomultiplier tube amplified raw output signal can in principle be captured directly by a personal computer sound card (PCSC) and processed by a lock-in emulated by software. This possibility is demonstrated in the present work and the performance of this new setup is compared with a traditional data acquisition system. The results obtained with this “PCSC and lock-in emulated by software” were of the same quality or even better compared to that obtained by conventional time integrators (Boxcars) and data acquisition boards. With PCSC the limits of detection (LOD) found for both naphthalene-2,3-dicarboxaldehyde-derivatized tyrosine and alanine were 3.3 and 3.5 fmol (injection of 5 nL of samples at 0.66 and 0.70 μmol/L), respectively. This is at least three times better compared to conventional systems when light emitting diodes (LEDs) are used as the excitation source in fluorescence detectors. The PCSC linear response range was also larger compared to conventional data acquisition boards. This scheme showed to be a practical and convenient alternative of data acquisition and signal processing for detection systems used in capillary electrophoresis.     

不同知识引出方法对科学概念结构表征的影响——来自多维标度法的证据

不同知识引出方式对获得的群体概念结构的影响,是概念结构研究中重要且未解决的问题.以17名中学化学教师为被试,通过自由分类、概念构图和语义相关性评定,引出被试对15个与原子相关概念的认识,并以多维标度法分析群体概念结构.研究发现:由不同知识引出方式获得的群体概念结构具有不同的特点,通过语义相关性评定获得的群体概念结构更适...     

Antioxidative and Anti-Inflammatory Phytochemicals and Related Stable Paramagnetic Species in Different Parts of Dragon Fruit

In this study, we investigated the antioxidant and anti-inflammatory phytochemicals and paramagnetic species in dragon fruit using high-performance liquid chromatography (HPLC) and electron paramagnetic resonance (EPR). HPLC analysis demonstrated that dragon fruit is enriched with bioactive phytochemicals, with significant variations between each part of the fruit. Anthocyanins namely, cyanidin 3-glucoside, delphinidin 3-glucoside, and pelargonidin 3-glucoside were detected in the dragon fruit peel and fresh red pulp. Epicatechin gallate, epigallocatechin, caffeine, and gallic acid were found in the dragon fruit seed. Additionally, 25–100 mg × L⁻¹ of dragon fruit pulp and peel extracts containing enrichment of cyanidin 3-glucoside were found to inhibit the production of reactive oxygen species (ROS), reactive nitrogen species (RNS), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in cell-based studies without exerted cytotoxicity. EPR primarily detected two paramagnetic species in the red samples. These two different radical species were assigned as stable radicals and Mn²⁺ (paramagnetic species) based on the g-values and hyperfine components. In addition, the broad EPR line width of the white peel can be correlated to a unique moiety in dragon fruit. Our EPR and HPLC results provide new insight regarding the phytochemicals and related stable intermediates found in various parts of dragon fruit. Thus, we suggest here that there is the potential to use dragon fruit peel, which contains anthocyanins, as a natural active pharmaceutical ingredient.     

RFID发展时代邮政业的挑战和机遇

本文介绍了邮政业应用电子标签的历史和应用中存在的问题及其解决办法,分析了第二代身份证和交通一卡通为其带来的新问题,最后指出了机场应用电子标签为邮政业带来的商机和挑战。     

一种基于IPSec协议的安全网卡的研究与设计

随着网络数据开放性,共享性的不断扩大,人们越来越重视网络传输的安全问题,针对这一问题,本文首先对IPSec协议族进行了研究,介绍了IPSec安全机制及应用,分析了认证头协议、封装安全载荷协议的结构及其关键技术。在此基础上提出了一种基于IPSec协议的安全网卡的设计模型,并阐述了此安全网卡的工作原理。     

IC卡式家用电度表设计

以单片机为核心,设计出IC卡式家用电度表,实现了预收费、报警、显示、断电和防作弊等功能。论文从硬件电路和软件设计两方面进行论述,并提出电度表的网络化管理思路。     

基于PCI总线的高速多路数据传输卡设计

介绍了一套基于外部设备互连(PCI)总线的高速多路数据传输卡的设计,采用基于PCI内核与PCI用户逻辑相结合的新型设计方案,在顶层通过仿真来验证PCI接口以及用户逻辑设计正确与否.降低了设计的复杂程度,提高了电路的集成度和系统的性能,并根据PCI卡对外部设备驱动能力较弱的特点,在传输卡中加入了长线驱动功能,采用低电压差分信号(LVDS)技术,既降低了系统功耗,又实现长距离的计算机双向通信.     

基于TC8830AF的智能语音卡结构与设计

本文描述一种基于８０３１单片机和ＴＣ８８３０ＡＦ语音处理器的ＰＣ机智能语音卡，通过软硬件设计解决了ＰＣ－８０３１－ＴＣ８８３０ＡＦ结构中的通讯问题。该语音卡在进行语音的合成、编辑和播放时，仅占用极少的ＰＣ机ＣＰＵ处理时间，能满足计算机系统进行实时语音合成的要求。