Affinity Chromatography of Insulin with a Heptapeptide Ligand Selected from Phage Display Library

A heptapeptide phage display library was screened with insulin to find its ligands for affinity chromatography. The peptide was synthesized and coupled to EAH Sepharose 4B (5.4 mol mL^–1 bed). Then, insulin chromatography was carried out with mobile phases of different pH values and by the addition of urea and ethylene glycol. It was found that electrostatic interactions were predominant for the affinity binding, and hydrogen bonding might also contribute somewhat to the affinity. Finally, frontal analysis was performed and the dynamic binding capacity of the affinity column for insulin at 50% breakthrough was estimated at 60.6 mg mL^–1 bed, which was about two times higher than the theoretical binding capacity of the monomeric insulin. The result suggests that insulin was bound in dimer state in a stoichiometric relationship with the coupled peptide, indicating the high binding efficiency of the peptide ligand for insulin.     

出租车司机社会贡献评价方法研究

出租车司机的社会贡献对于促进城市经济发展和社会进步具有重要的作用,评价出租车司机的社会贡献是激励出租车司机多做社会贡献的重要手段。为了评价出租车司机的社会贡献,本文提出了一种基于实际出租车运营数据的评价方法。本文设计了运营特性、重点区域、恶劣天气、大事件四个体现司机社会贡献的指标,以此构建了出租车司机社会贡献评价指标体系,进而选用了主观赋权法G1法和客观赋权法拉开档次法相结合的赋权方式对指标赋权,并选择四种评价信息集结方法建立了出租车司机社会贡献评价模型。最后,通过实际的出租车运营数据验证了本方法的可行性。本文为评价出租车司机社会贡献提供了一种研究思路和方法,并对政府政策的制定和企业策略的实施具有一定的启示。     

一种43 GHz超高带宽线性驱动电路设计

基于0.13μm SiGe BiCMOS工艺,设计了一种线性驱动电路。该电路具有高速和大摆幅的优势,能线性驱动行波马赫-曾德尔调制器(TW-MZM),可满足光通信系统100 Gbit/s单通道的应用需求。驱动电路包括连续时间线性均衡(CTLE)电路、可变增益放大(VGA)电路和基于Cascode结构改进优化的输出级电路,实现了增益可调,且避免发生由较大输出摆幅导致的晶体管击穿。仿真结果表明,电路的-3 dB带宽为43 GHz,其增益在15～25 dB内可调。在56 Gbaud NRZ/PAM4的输入信号下,测得的眼图形状良好,差分输出摆幅峰-峰值达4 V,电路整体功耗为1.02 W,面积为0.33 mm²。     

小型化低功耗机载显示器图形系统设计与实现

为了满足机载显示器图形生成系统的小尺寸、低功耗需求,提出了一种基于龙芯2K1000的全国产图形生成与处理方案。该方案以2K1000为核心构建硬件平台,使用2K1000内部集成的中央处理器、图形处理器、显示控制器,以及外部的内存实现图形的计算生成和双缓存显示,配合国产可编程逻辑器件实现图形与外视频的叠加显示及机载通信总线扩展。软件上采用航空专用的国产天脉操作系统,基于天脉操作系统设计了关键的图形显示驱动、帧存驱动、显示控制器驱动。实验结果表明,在输出1 024 pixel×768 pixel分辨率显示时,典型机载图形画面帧率达到35 frame/s,整体功耗约10 W。该方案扩展性强、功耗低,满足实时显示需求,在机载显示器领域有着广泛的应用空间。     

贝克曼离心机故障排除两例

简要介绍贝克曼离心机触摸键盘失控和控制参数显示混乱两例故障的故障现象、故障分析以及故障排除方法。     

不同光滑度立方体氧化铁对液晶电光性能的改善

向列相液晶被广泛应用于液晶显示中,但是由于杂质的存在,会导致液晶的驱动电压变大,增加能耗。 为了降低阈值电压和饱和电压,通常向液晶中添加纳米颗粒来提高电光性能。 本文利用水热法制备了表面粗糙和光滑的两种立方体Fe₂O₃纳米颗粒,其形貌均匀,尺寸约550 nm。 将二者分别掺杂到向列相液晶E7中,结果表明,粗糙立方体Fe₂O₃/E7复合体系具有比光滑立方体Fe₂O₃/E7复合体系和向列相液晶E7更优的电光性能,且在掺杂质量分数为0.4%时,其电光性能达到最优,阈值电压和饱和电压分别降低9.9%和11.6%,对比度增大80%,响应时间降低至6.0 ms。 这归因于粗糙立方体Fe₂O₃具有足够的表面积和表面所带电荷更多,所以会更易吸附体系中的杂质离子和减弱杂质离子的屏蔽作用,从而提高了电光性能。     

Color Displays with Gel-Glass Dispersed Liquid Crystals

Liquid crystal microdroplets trapped into silica gel-glasses by sol-gel processes may be used for display applications. Gel-glass dispersed liquid crystals (GDLC) are switched between opaque and transparent states by applying external AC-voltage signals; no polarizers are required. The feasibility of applying these materials to colored displays has been explored. A comparative study of different dyes for GDLC color displays, which were either embedded in the sol-gel matrix or dissolved in the liquid crystal microdroplets is presented.     

Capacity of Bacillus thuringiensis S-layer protein displaying polyhistidine peptides on the cell surface

S-layer protein of Bacillus thuringiensis strain CTC was used as the carrier protein to display polyhistidine (poly[6His]) peptides on the cell surface. Poly(6His) _n was fused with S-layer protein at two different sites, inserting just downstream of the S-layer protein homologous domain (slh) and replacing the non-slh region of S-layer protein, respectively. The two series chimeric proteins were both expressed by crystal negative B. thuringiensis strain 4Q7 and strain 171, respectively, as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The recombinant B. thuringiensis cells gained Ni²⁺- and Cd²⁺-binding ability and had a capacity to display up to nine copies of poly(6His). The Cd²⁺ adsorption quantity of the recombinant strain with the strongest adsorption ability was twice that of the host strain.     

Nucleophilic proteolytic antibodies

Proteolytic antibodies appear to utilizecatalytic mechanisms akin to nonantibody serine proteases, assessed from mutagenesis and protease-inhibitor studies. The catalytic efficiency derives substantially from the ability to recognize the ground state with high affinity. Because the proteolytic activity is germline-encoded, catalysts with specificity for virtually any target polypeptide could potentially be developed by applying appropriate immunogens and selection strategies. Analysis of transition-state stabilizing interactions suggests that chemical reactivity ofactive-site serine residues is an important contributor to catalysis. A prototype antigen analog capable of reacting covalently with nucleophilic serine residues permitted enrichment of the catalysts from a phage-displayed lupus light-chain library. Further mechanistic developments in understanding proteolytic antibodies may lead to the isolation of catalysts suitable for passive immunotherapy of major diseases, and elicitation of catalytic immunity as a component of prophylactic vaccination.     

New liquid‐crystal alignment agents based on fluorinated polyimides with trifluoromethyl‐substituted benzene or diphenylether in the side chain

Fluorinated copolyimides derived from 4,4′‐oxydiphthalic anhydride (ODPA) with 4,4′‐oxydianline (ODA) and trifluoromethyl‐containing aromatic diamines have been synthesized and characterized. The trifluoromethyl‐containing diamines include 2,4‐diamino‐3′‐trifluoromethylazobenzene, 2,4‐diamino‐1‐[(4′‐trifluoromethylphenoxy) phenyl] aniline, 3,5‐diamino‐1‐[(4′‐trifluoromethylphenoxy) phenyl] benzamide, 3,5‐diamino‐1‐[(3′‐trifluoromethyl) phenyl] benzamide, 1,4‐bis(4′‐aminophenoxy)‐2‐(3′‐trifluoromethylphenyl) benzene, 3,5‐diaminobenzenetrifluoride, 4,4′‐diamino‐4″‐(p‐trifluoromethyl phenoxy) triphenylamine, and 4‐[(4′‐trifluoromethylphenoxy) phenyl]‐2,6‐bis(4″‐aminophenyl)pyridine. Strong and flexible copolyimide films, produced by casting the polyamic acid solution followed by thermal imidization, exhibited great thermal stability and high mechanical properties. The polyimides had an ultraviolet–visible absorption cutoff at 330–340 nm and pretilt angles as high as 20° for nematic liquid crystals, making them great potential candidates for advanced liquid‐crystal display applications. © 2002 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 40: 1583–1593, 2002