浅议计算机网络安全工程

简要分析了计算机网络安全的一些实用技术，并提出了网络安全工程的实施对策。     

Atom-transfer radical polymerization of styrene with bifunctional and monofunctional initiators: Experimental and mathematical modeling results

Bulk atom transfer radical polymerization (ATRP) of styrene was carried out at 110 °C using benzal bromide as bifunctional initiator and 1-bromoethyl benzene as monofunctional initiator. CuBr/2,2′-bipyridyl was used as the ATRP catalyst. The polymerization kinetic data for styrene with both initiators was measured and compared with a mathematical model based on the method of moments and another one using Monte Carlo simulation. An empirical correlation was incorporated into the model to account for diffusion-controlled termination reactions. Both models can predict monomer conversion, polymer molecular weight averages, and polydispersity index. In addition, the Monte Carlo model can also predict the full molecular weight distribution of the polymer. Our experimental results agree with our model predictions that bifunctional initiators can produce polymers with higher molecular weights and narrower molecular weight distributions than monofunctional initiators. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 2212–2224, 2007     

杭州 市 区环境工程地质及软土地基稳定性评价

本文着重 对杭 州市环 境工程地 质特 点及其软土 地基稳定性 作出指导性评 价.对加强地下工程建设,城市 总体规划布局,城市详细 规划,单体 建筑设计和 施工,工程地 质勘 察等的宏观控制与微观指导,具有重要意 义     

Non-Linear Self-Excited Random Vibrations and Stability of an SDOF System with Parametric Noises

Abstract. The paper presents the solution to the properties of stochastic response of a system with random parametric noises, which is prone to the loss of aerodynamical stability. The system is described by an equation of van der Pol type with the negative linear, and with the positive cubic dampings. The coefficients of the linear damping and of the stiffness include the multiplicative random perturbations, the external excitation being given as a sum of a deterministic function and of an additive perturbation. All three input random processes are supposed to be Gaussian and centered, with the non-zero mutual stochastic parameters, as it corresponds to the properties of real systems. The solution has been based on the method of stochastic linearisation and of the subsequent solution of the Fokker–Planck–Kolmogorov equation in the sense of the first and second stochastic moments for the transient and stationary states. There have been demonstrated several effects, which are typical for systems with parametric noises, differentiating them from the systems with constant coefficients. The principal attention has been devoted to the properties of the spectral density of the response, the character of which changes abruptly with the degree of non-linearity of the damping and of the level of random perturbations.Sommario. La presente memoria studia le proprietà della risposta stocastica di un sistema con eccitazione casuale parametrica, che tende alla perdita della stabilità aerodinamica. Il sistema è descritto mediante un'equazione del tipo di van der Pole con il termine lineare dello smorzamento negativo e il termine cubico positivo. Poichá l'eccitazione esterna è la somma di una funzione deterministica e di una perturbazione additiva, i coefficienti dello smorzamento lineare e della rigidezza comprendono le perturbazioni casuali moltiplicative. I tre processi stocastici di eccitazione sono assunti gaussiani e a media nulla con parametri stocastici incrociati diversi da zero, come si verifica per le proprietà dei sistemi reali. La soluzione è basata sul metodo della linearizzazione stocastica e della successiva soluzione dell'equazione di Fokker-Planck-Kolmogorov studiando i primi e i secondi momenti statistici per gli stati transitori e stazionari. Vengono mostrati diversi effetti, tipici dei sistemi con eccitazione parametrica, differenziandoli dai sistemi a coefficienti costanti. Particolare attenzione è rivolta alle proprietà della densità spettrale della risposta le cui caratteristiche cambiano bruscamente con il grado di non linearità dello smorzamento e del livello di casualità delle perturbazioni.     

Structural Characterization of O‐ and C‐Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2

The structures of the O‐glycosyltransferase LanGT2 and the engineered, C? C bond‐forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide‐sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced‐fit transition was explored by molecular docking experiments with various aglycon substrates.     

Engineering Rieske Non‐Heme Iron Oxygenases for the Asymmetric Dihydroxylation of Alkenes

The asymmetric dihydroxylation of olefins is of special interest due to the facile transformation of the chiral diol products into valuable derivatives. Rieske non‐heme iron oxygenases (ROs) represent promising biocatalysts for this reaction as they can be engineered to efficiently catalyze the selective mono‐ and dihydroxylation of various olefins. The introduction of a single point mutation improved selectivities (≥95 %) and conversions (>99 %) towards selected alkenes. By modifying the size of one active site amino acid side chain, we were able to modulate the regio‐ and stereoselectivity of these enzymes. For distinct substrates, mutants displayed altered regioselectivities or even favored opposite enantiomers compared to the wild‐type ROs, offering a sustainable approach for the oxyfunctionalization of a wide variety of structurally different olefins.     

Electrochemical evidence for asialoglycoprotein receptor – mediated hepatocyte adhesion and proliferation in three dimensional tissue engineering scaffolds

Asialoglycoprotein receptor (ASGPR) is one of the recognition motifs on the surface of hepatocytes, which promote their adhesion to extracellular matrix in liver tissue and appropriate artificial surfaces. ASGPR-mediated adhesion is expected to minimize trans-differentiation of hepatocytes in vitro that is generally observed in integrin-mediated adhesion. The aim of the present study is to verify the role of ASGPR in hepatocyte adhesion and proliferation in scaffolds for hepatic tissue engineering. Scanning Electrochemical Microscopy (SECM) is emerging as a suitable non-invasive analytical tool due to its high sensitivity and capability to correlate the morphology and activity of live cells. HepG2 cells and rat primary hepatocytes cultured in Polyvinyl alcohol (PVA)/Gelatin hydrogel scaffolds with and without galactose (a ligand for ASGPR) modification are studied using SECM. Systematic investigation of live cells cultured for different durations in scaffolds of different compositions (9:1 and 8:2 PVA:Gelatin with and without galactose) reveals significant improvement in cell–cell communication and proliferation on galactose incorporated scaffolds, thereby demonstrating the positive influence of ASGPR-mediated adhesion. In this work, we have also developed a methodology to quantify the respiratory activity and intracellular redox activity of live cells cultured in porous tissue engineering scaffolds. Using this methodology, SECM results are compared with routine cell culture assays viz., MTS ((1-Oxyl-2,2,5,5,-tetramethyl-Δ3-pyrroline-3-methyl) Methanethiosulfonate) and Albumin assays to demonstrate the better sensitivity of SECM. In addition, the present study demonstrates SECM as a reliable and sensitive tool to monitor the activity of live cells cultured in scaffolds for tissue engineering, which could be used on a routine basis.     

位移时空综合分析法及其在边坡工程中的应用

以往对监测位移数据分析方法主要是位移-时间关系分析。但监测数据包含了大量对工程设计和施工都很有用的信息，其中不乏位移-空间方面的信息；另一方面，从工程设计和施工角度看，除需要位移-时间信息外，还需要位移-空间信息。位移时空综合分析法的提出旨在综合位移-时间和位移-空间等两方面的信息，为工程设计和施工服务。该方法依表现形式不同又分为竖线法和曲面法两种。本文将通过五强溪船闸边坡工程实例，来进一步说明位移时空综合分析法的原理及其应用。     

Active Longitudinal Control of Wind-Induced Oscillations of a Suspended Cable

Abstract. Active control of oscillations of a suspended cable under transversal wind, described by a constant term and a fluctuating turbulent component, is dealt with. Control of transversal in-plane and out-of-plane oscillations is performed by imposing a longitudinal motion to one support; the control law depends, linearly or quadratically, on measures of displacement and velocity of a selected point. First, the steady-state oscillations around the planar static configuration, determined by the wind constant component, under sinusoidal in-plane and out-of-plane loading are studied, showing the nonlinear behaviour in the regions of primary and parametric resonance and the effects of the control action. Afterwards, attention is focused on the response of the cable to transversal wind turbulence, whose fluctuating component is described by a random process with an assigned spectral density. The effectiveness of longitudinal control is analysed by means of numerical investigations.Sommario. Si analizza il controllo attivo delle oscillazioni di un cavo sospeso, sottoposto all'azione di un vento agente in direzione ortogonale al piano del cavo e descritto mediante un termine di carico costante ed una componente variabile di turbolenza. Il controllo delle oscillazioni piane e spaziali viene realizzato mediante spostamento longitudinale di uno degli appoggi; la legge di controllo dipende linearmente o quadraticamente da misure di spostamento e velocità di un punto prescelto. In una prima fase, vengono studiate le oscillazioni stazionarie sotto carichi sinusoidali agenti rispettivamente nel piano e fuori del piano del cavo, intorno alla configurazione di equilibrio statico determinata dalla presenza della componente costante del vento, mostrando il comportamento nonlineare nelle regioni di risonanza primaria e parametrica e gli effetti dell'azione di controllo. Successivamente, si focalizza l'attenzione sulla risposta del cavo alla componente trasversale di turbolenza del vento, la quale è descritta da un processo stocastico a media nulla con densità spettrale assegnata. L'efficacia del controllo viene analizzata attraverso un'indagine numerica.