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A simple analytical system using disposable, open-tubular ion exchange clean-up precolumns coupled in-line to capillary electrophoresis for direct injection of biological samples is presented. The clean-up precolumns were prepared from fused silica capillaries by thermally initiated layer-by-layer polymerization of poly(butadiene-maleic acid) (PBMA) directly on the capillary wall. Typically, 6 cm long precolumns with 4-layers of PBMA were used for sample pretreatment. A robust and reproducible coupling between the precolumn (75 μm ID) and the analytical capillary (50 μm ID) was achieved using an inexpensive, commercially available low dead volume union. No extra dispersion of the analyte zones was observed. Proteins and other high molecular weight compounds from biological sample matrices were retained on the cation-exchanger sites of the precolumn, which eliminated their adsorption on analytical capillary walls and ensured stable electroosmotic flow and migration times of target analytes. Unretained small inorganic cations migrated freely into the analytical capillary for separation and detection. Applicability of the sample clean-up procedure was proved by determination of major inorganic cations in blood serum and plasma samples using capillary electrophoresis with contactless conductivity detection. Separations were performed in background electrolyte solution consisting of 15 mM L-arginine, 12.5 mM maleic acid, 3 mM 18-crown-6 at pH 5.5 and repeatabilities of migration times and peak areas were below 1.5% and 7.3%, respectively. Less than 1 μL of biological sample was required for injection. 相似文献
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Shui Feng ZHANG Li Shi WANG Zhi DANG 《中国化学快报》2006,17(9):1229-1232
This paper reported a compact system of capacitively coupled contactless conductivity detection (C4D) based on the square wave excitation voltage for capillary electrophoresis, and it exhibited excellent sensitivity at the optimal frequency of 198 kHz. The feasibility and sensitivity of this detector was demonstrated by simultaneous detection of thirteen ions including alkali, alkaline earth and heavy metal ions. And the detection limits (S/N 3) were in the range of 0.2-1μmol/L for Mn^2+, K^+, Na^+, Mg^2+, Ca^2+, Li^+, Ba^2+, and 7-25 μmol/L for Ni^2+, Cu^2+, Cd^2+, Pb^2+, Co^2+, Zn^2+. 相似文献
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A study on the determination of the antibiotic tobramycin by CE with capacitively coupled contactless conductivity detection is presented. This method enabled the direct quantification of the non-UV-absorbing species without incurring the disadvantages of the indirect approaches which would be needed for optical detection. The separation of tobramycin from inorganic cations present in serum samples was achieved by optimizing the composition of the acetic acid buffer. Field-amplified sample stacking was employed to enhance the sensitivity of the method and a detection limit of 50 microg/L (S/N = 3) was reached. The RSDs obtained for migration time and peak area using kanamycin B as internal standard were typically 0.12 and 4%, respectively. The newly developed method was validated by measuring the concentration of tobramycin in serum standards containing typical therapeutic concentrations of 2 and 10 mg/L. The recoveries were 96 and 97% for the two concentrations, respectively. 相似文献
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The separation and detection of commonly used preservatives (benzoate, sorbate) and vitamin C by both conventional CE and microchip electrophoresis with capacitively coupled contactless conductivity detection is presented. The separation was optimized by adjusting the pH-value of the buffer and the use of hydroxypropyl-beta-CD (HP-beta-CD) and CTAB as additives. For conventional CE, optimal separation conditions were achieved in a histidine/tartrate buffer at pH 6.5, containing 0.025% HP-beta-CD and 0.1 mM CTAB. LOD ranged from 0.5 to 3 mg/L (S/N = 3) and the RSDs for migration time and peak area were less than 0.1 and 2%, respectively. A considerable reduction of analysis time can be accomplished by using microchip electrophoresis without significant loss in sensitivity under optimal separation conditions. A histidine/tartrate buffer at pH 6.5, incorporating 0.06% HP-beta-CD and 0.25 mM CTAB, gave detection limits ranging between 3 and 10 mg/L and satisfactory reproducibilities of < or =0.4% for the migration time and < or =3.5% for the peak area. The methods developed are useful for the quantitative determination of food additives in real samples such as soft drinks and vitamin C tablets. 相似文献
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生物组织电导率CMIT平面螺旋激励线圈的分析 总被引:3,自引:0,他引:3
计算了平面矩形螺旋线圈的电感值,结果与近似公式计算得出的电感值吻合。另外,通过理论计算发现,矩形平面螺旋线圈产生的磁场具有聚焦的特性,并计算和讨论了分层生物组织的电导率对电感的影响,认为用矩形平面螺旋线圈来做生物组织非接触的电导率成像的激励线圈是可行的。 相似文献
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介绍了我国移动支付技术和产业发展状况,重点阐释了国家标准制定情况。并对我国移动支付产业发展和标准化工作提出了建议。 相似文献
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