Determination of Vitamin C in Plasma and Dialysate from Uremia Patientsby High Performance Liquid Chromatography with Electrochemical Detection

 Abstract：Aconvenientandvalidmethodforthedeterminationofascorbicacid(AA)anddehydroascorbicacid(DHAA)inplasmaanddialysatefrompatientswithuremiabyhighperformanceliquidchromatographywithelectrochemicaldetectionisdescribedAmixtureof08g/Lmetaphosphoricacidand     

离子色谱-积分脉冲安培检测法测定大蒜多糖的单糖组成

采用氨基酸分离柱(Amino PAC PA10,2×250 mm),用10.0 mmol/L NaOH溶液作为流动相,以Au为工作电极,Ag/AgCl为参比电极的脉冲积分安培离子色谱法,分离检测了大蒜多糖水解产生的单糖成分及相对含量。实验结果表明,大蒜多糖中含有半乳糖、葡萄糖、甘露醇和果糖,检出限在0.3~6μg/L范围,样品加标回收率为95%~112%。该法具有灵敏度高、精密度好、分离效果好和样品不需要衍生处理的优点,适用于各种植物多糖的单糖组成分析。     

便携式病害猪肉速测仪的研制与应用

基于健康与病害猪肉中过氧化物酶(POD)的活性不同的原理,研制出便携式的病害猪肉速测仪,用于病害猪肉的检验与鉴别,以评判猪肉的品质。仪器的工作系统由检测系统和软件系统两部分组成。该检测仪具有操作简便,检测快速,可进行现场检测等优点。在对市场猪肉的检测中,利用测定体系与POD的反应动力学曲线,可快速准确地完成病害猪肉的鉴别,健康猪肉和病害猪肉的符合率分别为100%和96%。     

基于偶氮水杨醛酰腙的氰根比色检测探针的合成及性能

设计合成了一种基于酚羟基和氨基的酰腙类探针分子, 利用紫外-可见吸收光谱和核磁滴定考察了其对F^-, Cl^-, Br^-, I^-, CH₃COO^-, H₂PO₄^-, HSO₄^-, ClO₄^-, CN^-, SCN^-, SO^2-₄和NO^-₃等阴离子的识别作用. 结果表明, 当加入CN^-离子时主体溶液颜色由无色变为黄色, 而加入其它离子时主体溶液颜色不变, 说明该探针在DMSO/H₂O(体积比5: 5)体系中能选择性裸眼比色检测CN^-. 核磁滴定及质谱数据表明, 该探针与CN^-以1:1化学计量比结合, 该过程通过亲核加成方式完成.     

LED光诱导化学发光法检测饮料中的核黄素

以化学发光法为基础,建立了以发光二极管(LED)诱导化学发光体系(LED-CL)检测饮料中核黄素含量的分析方法。样品溶液与鲁米诺溶液混合后由蠕动泵带出,经LED灯照射后产生化学发光,产生的化学发光信号由光电检测器检测。核黄素浓度检测线性范围为0.39~79.56μg/L(R≥0.999 7),加标回收率为99.3%~103%,可用于饮料中核黄素的检测。     

食品安全分析样品前处理-快速检测联用方法研究进展

综述了近年来传感器、可视化分析、便携光谱、酶联免疫检测及便携气相色谱等食品安全快速检测技术的研究现状,以及食品安全分析中样品前处理-快速检测联用技术的研究进展,为发展选择性好、准确度高、可定量的新型食品安全快速检测技术提供参考。     

Malathion detection method using microhotplate-based preconcentrator and ion mobility spectrometer

A simple and rapid method using a microhotplate-based preconcentrator and an ion mobility spectrometer (IMS) is proposed for the detection of malathion in water. The preconcentrator is prepared by micro-electro-mechanical system (MEMS) process. Coated with Polydimethylsiloxane (PDMS), it has the advantages of solvent-less, low energy cost, self-heating and ease to combine with IMS. The operating conditions of the preconcentrator-IMS system, such as extraction time, extraction temperature, agitation speed and desorption temperature, were optimised. Using the preconcentrator, the sampling procedure can be simplified and the detection limit of the system can be decreased. A linear relationship between the IMS response and the concentration of the analyte solution was verified. The malathion detection limit based on 3 times the baseline noise is 0.43?µg?L^?1 and the total analysis time is less than 30?minutes.     

微囊藻毒素检测方法研究进展

综述了目前国内外微囊藻毒素检测方面的各种研究方法及成果,对高效液相色谱(HPLC)、液相色谱串联质谱(LC–MS)、气相色谱串联质谱(LC–MS)、毛细管电泳(CE)、薄层色谱(TLC)、生物法和生物化学法等检测方法存在的优缺点进行了评述,并展望了该研究领域的未来发展趋势。     

Rapid Sample Pretreatment,Identification and Determination of Active Constitutents in Water‐soluble Radix isatidis Extract via MAE,ESI‐MS and RODWs‐HPLC

In this study, we successfully studied water‐soluble extract from Radix isatidis. Optimized conditions of MAE were listed, the sample can be extracted completely in 10 minutes under microwave power of 400W and solid/liquid ratio of 1:80. Active compounds in water‐soluble extract from R. isatidis were identified with HPLC‐DAD/ESI‐MS, these compounds followed by cytidine, uridine, guanosine, (R,S)‐goitrin and adenosine. RODWs–HPLC as a new sensitive chromatography were also first proposed and investigated, we favoringly used this method for simultaneous determination of these active constitutents in water‐soluble R. isatidis extract. Chromatographic separation was performed on a Diamonsil C18 column (5 μm, 150 mm × 4.6 mm) with a mobile phase gradient consisting of methanol and water at a flow‐rate of 1.0 mL/min, detection wavelengths 240, 250, 260 and 270 nm, the retention times of the tested five compounds were about 4.2, 5.8, 11.1, 14.2 and 20.8 min respectively, the limits of detection were 15, 12, 20, 5.8 and 24 ng/mL for cytidine, uridine, guanosine, (R,S)‐goitrin and adenosine respectively, their linear ranges were between 0.045 and 350 μg/mL with correlation coefficient (R) of 0.9998‐0.9999. The relative standard deviations (RSDs) of intra‐day and inter‐day assays were 0.30‐2.36% and 0.86‐2.54% respectively. Extraction recoveries were 94.25‐106.21%. This novel analytical method was shown to be simple, low‐cost, sensitive and reliable for multiple components in complex or undeveloped materials via MAE, ESI‐MS and RODWs‐HPLC.