Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry

Hydrophilic interaction liquid chromatography (HILIC) is here successfully coupled to negative-ion electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) for the analysis of synthetic and chemically modified oligonucleotides. Separation was performed on a 2.1 mm × 100 mm PEEK ZIC^® HILIC column packed with hydrophilic stationary phase with a permanent zwitterionic functional group and a particle size of 3.5 μm with an average pore diameter of 200 Å. A method was developed to separate homogeneous and heterogeneous oligonucleotides as well as methylated oligonucleotides using a quaternary pumping system containing ammonium acetate and water with an acetonitrile gradient. Analyses of oligonucleotides were performed by LC/MS with a detection limit of 2.5 picomole (20 mer) with signal to noise ratio (S/N) of 4.12. The influence of the eluent composition, type of buffer and its concentration, and organic modifier were also evaluated. The HILIC LC/MS method presented in this paper used common, ‘MS friendly’, mobile phases achieving sensitive and selective oligonucleotide analysis.     

Semi-synthetic polysaccharide sulfates as anticoagulant coatings for PET, 1--cellulose sulfate

In the present study, blood-compatible PET surfaces were prepared by coating with anticoagulant cellulose sulfates that were synthesized homogeneously in ionic liquids. The adsorption behavior of polysaccharides on PET films was investigated using QCM-D. It was demonstrated that pre-coating with different amino-group-containing polysaccharides improves the affinity toward cellulose sulfate. Moreover, the effect of different degrees of sulfation on the adsorption process was evaluated. Based on these results, several layer-by-layer coated PET foils were prepared that showed significantly improved blood compatibility compared to the initial untreated material.     

Layer-by-layer assembled multilayer films of methoxypoly(ethylene glycol)-block-poly(α,l-glutamic acid) and chitosan with reduced cell adhesion

A methoxypoly(ethylene glycol)-block-poly(α,L-glutamic acid) (mPEGGA) diblock copolymer is synthesized. Using QCM measurements, it is shown that (CS/mPEGGA)(n) film construction takes place over two build-up stages (exponential-to-linear). UV-vis spectra reveal the regular increase of the multilayer film growth at different molecular weights of mPEGGA. Contact angle and surface morphology investigation prove that the hydrophilicity of CS/mPEGGA multilayer film-modified substrate becomes better and the surface becomes rough. Significantly reduced cell adhesion is observed on the CS/mPEGGA multilayer film coated surface.     

On a rational differential quadrature method in irregular domains for problems with boundary layers

A rational differential quadrature method in irregular domains (RDQMID) is investigated to deal with a kind of singularly perturbed problems with boundary layers. Through a transformation, the boundary layer, which may be not straight, is transformed into a segment of a line parallel to one of the Cartesian axes. The rational differential quadrature method (RDQM) is applied to discretize the governing equation. Finally, a direct expansion method of the boundary conditions (DEMBC) is raised to deal with the boundary conditions. Numerical experiments show that RDQMID is of high accuracy, efficiency and easy to programme.     

Approximate analytical solutions of a class of boundary layer equations over nonlinear stretching surface

Third order nonlinear ordinary differential equations, subject to appropriate boundary conditions arising in fluid dynamics, are solved using three different methods viz., the Dirichlet series, method of stretching of variables, and asymptotic function method. Similarity transformations are used to convert the governing partial differential equations into nonlinear ordinary differential equations. The numerical results obtained from the above methods for various problems are given in terms of skin friction. Our study revealed that the results obtained from these methods agree well with those of direct numerical simulation of ordinary differential equations. Also, these methods have advantages over pure numerical methods in obtaining derived quantities such as velocity profile accurately for various values of the parameters at a stretch.     

Frictionless elliptical contact of thin viscoelastic layers bonded to rigid substrates

A three-dimensional unilateral contact problem for thin viscoelastic layers bonded to rigid substrates shaped like elliptic paraboloids is considered. Two cases are studied: (a) Poisson’s ratios of the layer materials are not very close to 0.5 and (b) the layer materials are incompressible with Poisson’s ratio of 0.5. Poisson’s ratios are assumed to be time independent. In the present paper we derive the general solutions to the problems of elliptical contact between thin compressible or incompressible layers of arbitrary viscoelastic materials. The approach is based on the analytical method developed by the authors for the elliptical contact of thin biphasic cartilage layers. The obtained analytical solution is valid for monotonically increasing loading conditions.     

转捩边界层中次生流向涡演化的数值研究

采用高精度直接数值模拟方法和高效的特征无反射边界条件,进行可压缩流转捩边界层中出现的次生流向涡演化的数值研究.精细的数值模拟结果清楚地揭示了转捩边界层的复杂流场中次生流向涡的形成和演化过程,探讨它对转捩至关重要的环状涡生成的影响,发现次生流向涡和主流向涡的共同作用形成环状涡的一种新机理.     

TD-Femtocell协议栈RLC层AM模式数据传输过程分析

无线链路控制（RLC）子层位于TD-SCDMA系统无线接口协议栈的第二层,它向高层提供三种数据传输模式：透明模式（TM）,非确认模式（UM）和确认模式（AM）。在主要介绍无线接入网空中接口协议栈结构的同时,详细阐述了RLC层AM数据传输模式的运行原理和具体在协议栈中实现AM传输模式的方法。     

Traffic‐aware downlink power allocation in multiuser OFDM system

Multiuser orthogonal frequency division multiplexing (OFDM) is a promising technology to achieve high uplink/downlink (DL) capacities in the next generation broadband wireless networks such as WiMAX (Worldwide Interoperability for Microwave Access). In this paper, we investigate the DL adaptive power allocation (APA) in multiuser OFDM system from the perspective of cross‐layer design. Specifically, we formulate APA as an optimization problem with the traffic profile of each user asit a priori knowledge. To solve the optimization problem, we develop a fairness‐constrained optimal prioritized effective throughput (PET) strategy and the corresponding iterative algorithms, aiming at balancing the prioritized effective throughput and the linear or logarithmic user satisfaction‐based fairness. Simulation results show that our proposed APA optimization approach can achieve satisfying performance. Copyright © 2011 John Wiley & Sons, Ltd.     

Cross‐layer and cognitive QoS management system for next‐generation networking

Owing to limited wireless network resources, network applications must provide an adaptive quality‐guaranteed service to satisfy user requirements. Different applications are associated with different quality of service (QoS) concerns, as well as different QoS control parameters. This work presents an adaptive QoS algorithm by discussing the QoS specifications of three wireless access technologies, i.e. 3G, WiMAX and WiFi. Based on cross‐layer and cognition concepts, these environmental parameters are integrated with the sensing of spectral and received signal strength from a cognitive radio paradigm. An adaptive QoS algorithm is then proposed to select the optimal access network for services. Simulation results indicate that the proposed adaptive QoS algorithm outperforms available ones in real‐time applications. Compared with traditional algorithms, the proposed algorithm reduces not only the average delay time and jitter for VoIP services to 0.16 s and 0.09 ms, respectively, but also the packet loss ratio for high‐definition video streaming by 3.4%. Copyright © 2011 John Wiley & Sons, Ltd.