A Small-Molecule Diketopyrrolopyrrole-Based Dye for in vivo NIR-IIa Fluorescence Bioimaging

Organic small-molecule fluorophores with near-infrared IIa (NIR-IIa) emission have great potential in pre-clinical detection and inoperative imaging due to the high-spatial resolution and deep penetration. However, developments of the NIR-IIa fluorophores are still facing considerable challenges. In this work, a series of diketopyrrolopyrrole (DPP)-based fluorophores were designed and synthesized. Subsequently, nanomaterial T25@F127 with significant NIR-IIa emission properties was rationally prepared by encapsulating DPP-based fluorophore T25 , and was selected for fluorescence angiography and cerebral vascular microscopic imaging with nearly 800 μm penetrating depth and excellent signal-background ratio of 4.07 and 2.26 (at 250 and 400 μm), respectively. Furthermore, the nanomaterial T25@cRGD with tumor targeting ability can image tiny metastatic tumor on intestine with a small size of 0.3 mm×1.0 mm and high-spatial resolution (SBR=3.84). This study demonstrates that the nanomaterials which encapsulated T25 behave as excellent NIR-IIa fluorescence imaging agents and have a great potential for in vivo biological application.     

Polyoxometalate-Based Nanomaterials Toward Efficient Cancer Diagnosis and Therapy

As an emerging class of inorganic metal oxides, organically functionalized polyoxometalates (POMs) or POM-based nanohybrids have been demonstrated promising potential for the inhibition of various cancer types by the virtue of their diversity in structures and significantly reduced toxicity. This contribution summarizes the latest achievement of POM-based nanomaterials in cancer diagnosis and various therapeutics to put forward our fundamental viewpoints on the design principles of modified POMs based on their application. In addition, major challenges and perspectives in this field are also discussed. We expect that this review will provide a valuable and systematic reference for the further development of POM-based nanomaterials.     

一种比较器电路的自动失调补偿方法

文章提出了一种适用于高精度传感器和高精度模数转换器的比较器电路。该电路利用失调自动补偿技术提高了比较器的精度,该补偿技术不需要增加前置放大器的增益,也不需要增加静态电流,从而获得低噪声和低功耗。电路设计和Hspice仿真基于CSMC0．5μm CMOS工艺,电源电压3．3V。仿真结果表明,在时钟为100kHz、电源电压为3．3V下补偿之后的比较器的失调较补偿前由原来的9．75mV降低为0．31mV。     

多微机联控在大型三面翻广告牌中的应用

多微机联控系统应用不同类型的单片机,构成一个主控制器和多个现场控制器,充分发挥不同类型单片机的长处以提高系统的实用性和性价比。主控制器和多个现场控制器之间采用异步串口通信或“软串口”按照“通信协议”实现分支通信,组成多微机联控的“集散式控制”系统,按三面翻牌面翻转的控制要求分段驱动电机,实现大型三面翻牌面的定画面、定时间、可选翻转方式、出错牌面的“自动纠错翻转”等驱动要求。多微机联控系统极大地简化了控制电路的连线,简化了电气控制安装工艺与施工,在降低工程费用和后期维修费用的同时,可大幅提高控制系统工程的可靠性、稳定性、性价比。     

移动多媒体广播复用器自动热备份系统设计

论述了移动多媒体广播前端系统中设计自动热备份系统的重要意义,提出了复用器热自动备份系统的技术要求、系统结构,最后详细介绍了自动热备份的实施原理,包括切换条件以及切换过程中的信息交互和动作过程.     

Automatic Headspace Sampling for Determination of Ethylene Dibromide Residues in Cereals

Abstract

The possibility was investigated to apply a commercially available automatic head-space sampler in trace analysis of ethylene dibromide (EDB, 1,2-dibromoethane), in cereals.

Samples of rice and wheat flour were thermostatted in closed vials at 70[ddot]C for 30min. The top gas was then automatically introduced into a gas chromatograph equipped with an electron capture detector. Quantitation was performed using external standards (untreated samples spiked with solutions of EDB in N,N′-dimethylacetamide).

The relative standard deviation of the method was 3.4% for rice and 4.5% for wheat flour, at a residue level of 0.008 mg/kg. The detection limit was 0.001 mg/kg (the official EC residue tolerance is 0.01 mg/kg).

Preliminary experiments with other fumigants were carried out as well.

The headspace technique in question has the following advantages over other methods for determining EDB residues in cereals: 1. No sample pre-treatment like extraction, steam distillation, purge and trap etc.; 2. Automated sample handling; 3. “Clean” chromatograms.     

基于JC-5600ATE的单/双电源运算放大器测试方法

集成运算放大器具有成本低、功能多等优点,可广泛用于自动控制系统、测量仪表等其他电子设备中。近年来集成运放的指标在不断提高,性能也在不断提升,伴随着运算放大器的发展,对用来考核运算放大器指标的测试技术也提出了相应的要求,对测试系统(Automatic Testing Equipment,以下简称ATE)的性能要求也相应变高。阐述了单、双电源集成运算放大器在JC-5600ATE上的测试方法,并分析了两者的测试差异。     

基于小波分析的蜗轮蜗杆减速器故障诊断系统

随着蜗轮蜗杆减速器的广泛应用，其故障类型表现为复杂化和隐蔽化，这不仅会带来巨大的经济损失，甚至威胁工人的生命安全。为解决这一问题，提出了基于小波分析的蜗轮蜗杆减速器故障诊断系统，系统在模拟减速器真实工作情况的基础上，采用LabView编写故障诊断系统，实现对振动信号进行采集与显示、小波分析以及离线故障诊断，实验结果表明，文中提出基于小波分析的蜗轮蜗杆减速器故障诊断系统，能够较好地实现蜗轮蜗杆减速器故障的实时诊断，适用于蜗轮蜗杆减速器技术研究和安全应用等领域。     

Automated chemical resonance generation and structure filtration for kinetic modeling

This work discusses efficient and automated methods for constructing a set of representative resonance structures for arbitrary chemical species, including radicals and biradicals, consisting of the elements H, C, O, N, and S. Determining the representative reactive structures of chemical species is crucial for identification of reactive sites and consequently applying the correct reaction templates to generate the set of important reactions during automated chemical kinetic model generation. We describe a fundamental set of resonance pathway types, accounting for simple resonating structures, as well as global approaches for polycyclic aromatic species. Automatically discovering potential localized structures along with filtration to identify the representative structures was shown to be robust and relatively fast. The algorithms discussed here were recently implemented in the Reaction Mechanism Generator (RMG) software. The final structures proposed by this method were found to be in reasonable agreement with quantum chemical computation results of localized structure contributions to the resonance hybrid.     

Chitosan/Ce(IV) redox polymerization‐based amplification for detection of DNA point mutation

Polymerization‐based signal amplification, a technique developed for use in rapid diagnostic tests, hinges on the ability to localize initiators as a function of interfacial binding events. We report here a new DNA detection method in which polymer growth in redox‐polymerization is used as a means to amplify detection signals. The introduction of biotin‐labeled chitosan (biotin‐CS) with highly dense amino groups into the polymerization amplification as macromolecular reducing agent, beneficially simplifies amplification operation, as well as, provides a large amount of initiation points to improve the sensitivity of detection. DNA hybridization, SA and biotin binding reactions led to the attachment of CS on a solid surface where specific DNA sequences were located. With the addition of the mixture containing monomer AM, crosslinker PEGDA and oxidant CAN onto the CS location, the growth of polymer films was triggered to render the corresponding spots readily distinguishable to the naked eye. Direct visualization of 0.21 fmol target DNA molecules of interest was demonstrated. Non‐small cell lung cancer p53 sequence was further selected as a proof‐of‐principle to detect DNA point mutation. The proposed method exhibited an efficient amplification performance for molecule detection, and paved a new way for visual diagnosis of biomolecules. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54, 1929–1937