毛细管气相色谱法同时测定水产品中的多氯联苯和有机氯农药残留量

建立了毛细管气相色谱法同时测定水产品中多氯联苯和有机氯农药的方法，用高氯酸与冰乙酸的混合溶液消化样品，以石油醚提取，浓硫酸净化后，用毛细管气相色谱法测定，方法的回收法为88％－110％，相对标准偏差为8％－19％（n=6)，检出限为0．08－0．60ug/kg，方法简便，快速，结果准确。     

Application of recombinant phage display antibody system in study of Codakia orbicularis gill proteins

We used the recombinant phage display antibody system (RPAS) to obtain chimeric single-chain fragment variable (ScFv) antibodies to gill proteins of the white clam Codakia orbicularis (Linné, 1758). After three rounds of selection on immunotubes loaded with total gill protein extract, recombinant phages exhibiting antibodies to gill proteins were isolated and tested by enzyme-linked immunosorbent assay (ELISA). Clones exhibiting a high affinity for the mollusk proteins were selected for production of soluble ScFv antibodies, which were purified for subsequent analysis. ScFv antibodies exhibited a reaction specific for a protein whose molecular mass was about 15,000 Daltons and that was detected by the antigen capture technique followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting.     

Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time

The dynamics of gel isoelectric focusing were studied by using amphoteric low-molecular-mass colored substances (isoelectric point markers). The polyacrylamide gel in slab format was in direct contact with the electrodes. In addition to isoelectric focusing with a pH gradient composed of synthetic carrier ampholytes, pH gradients created by simple buffers of acetic acid, 2-(N-morpholino)ethanesulfonic acid, histidine and N,N,N',N'-tetramethylethylenediamine were applied. The progress of the electrofocusing process was monitored by a charge-coupled device camera and video recording. The gradient profile and dynamics were approximated from the positions of isoelectric point markers, which were focused both on boundaries between individual zones of simple buffers and within the zones themselves. The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing.     

Eliminating bandwidth estimation from adaptive video streaming in wireless networks

Dynamic Adaptive Streaming over HTTP (DASH) is the state-of-the-art technology for video streaming and has been widely deployed in both wired and wireless environments. However, mobile DASH users often suffer from video quality oscillation and even video freeze in wireless environments, which results in poor user experience. This is mainly because most quality adaptation algorithms in DASH rely highly on bandwidth estimation to adjust the video quality while wireless network bandwidth is unstable in nature and changes frequently according to wireless channel contention and condition. To provide stable performance, even during severe bandwidth fluctuation, this paper proposes the Wireless Quality Adaptation (WQUAD) algorithm, which eliminates bandwidth estimation from quality adaptation. Thanks to the Scalable Video Codec (SVC), the proposed scheme always prioritizes to lower layers over higher ones as long as the play-out buffer is not completely filled by the lower layers. As a result, the client always fills the buffer with the base layers first and then the upper enhancement layers sequentially. This horizontal adaptation is straightforward and does not require any bandwidth estimation. Through NS-2 simulations, we show that WQUAD achieves (i) stable performance, keeping the video quality level with respect to the long-term network bandwidth, (ii) effective video freeze prevention, and (iii) high video quality on average.     

A novel video abstraction method based on fast clustering of the regions of interest in key frames

Online video nowadays has become one of the top activities for users and has become easy to access. In the meantime, how to manage such huge amount of video data and retrieve them efficiently has become a big issue. In this article, we propose a novel method for video abstraction based on fast clustering of the regions of interest (ROIs). Firstly, the key-frames in each shot are extracted using the average histogram algorithm. Secondly, the saliency and edge maps are generated from each key-frame. According to these two maps, the key points for the visual attention model can be determined. Meanwhile, in order to expand the regions surrounding the key points, several thresholds are calculated from the corresponding key-frame. Thirdly, based on the key points and thresholds, several regions of interest are expanded and thus the main content in each frame is obtained. Finally, the fast clustering method is performed on the key frames by utilizing their ROIs. The performance and effectiveness of the proposed video abstraction algorithm is demonstrated by several experimental results.     

Web服务技术-大数据抓取及存储的研究与实现

简述web服务的关键技术以及其发展的过程,并对一些关键技术进行阐述,web服务是有自己独特的体系,拥有自己的一套架构,并且在这套架构上,web服务拥有自己的核心技术,当单个的web服务难以满足需求的时候,这时候web服务组合就出现了,它能够很好的解决单个web所不能解决的问题,这个很好的满足了现代社会的需求,最后提出web服务技术的未来,其中重点是说明web服务的核心技术。随着网络的兴起,web服务技术被广泛应用于大数据的海洋抓取,比如代购网站中,就需要用到数据的海洋抓取和存储。     

A real-time dual watermarking algorithm of H.264/AVC video stream for Video-on-Demand service

A real-time dual watermarking algorithm of H.264/AVC compressed video is proposed for Video-on-Demand (VOD) service. The copyright information and user information are modulated by CDMA spreading strategies as watermark. At the encoder side, copyright information is embedded into the first non-zero coefficient of Intra 4 × 4 coded blocks in the luminance components of I frames. An effective error compensation mechanism is simultaneously introduced into the embedding process, which strictly restricts the distortion caused by quantization and watermark embedding within a step size of quantization. At the server side, a XOR based mapping rule is utilized for watermark embedding because the number of non-zero coefficients after quantization is quite less in P frames. Every two bits of the private user information is mapped to three non-zero coefficients of P frames. Experimental results on test sequences demonstrate that the proposed approach has just slight influence on bit rate and PSNR with real-time performance for VOD services, and achieves the dual purposes of copyright protection and pirate tracking.     

Adaptive multimedia streaming using a simulation test bed

The major purpose of this paper is to transmit an existing video above a complete simulated video streaming architecture based on Network Simulator (NS2). Thanks to our architecture, the visual quality evaluation of the distributed streaming platform under various conditions is simplified. Indeed, the received video can be easily visualized using a classic video client or be compared using the Peak Signal-Noise Ratio (PSNR) value and the Structural SIMilarity (SSIM) value. In the case study, we compare adaptive video congestion strategies using a transcoder, Datagram Congestion Control Protocol (DCCP) and TCP-Friendly Rate Control (TFRC).     

Understanding Why Scholars Hold Different Views on the Influences of Video Games on Public Health

Despite decades of research, no scholarly consensus has been achieved regarding the potential impact of video games on youth aggression or other public health concerns. In recent years, hypotheses have been raised that scholarly opinions on video games may resemble past moral panics, with attitudes reflective of generational conflicts. These hypotheses are tested in a sample of 175 criminologists, psychologists, and media scholars, examining both overall negative attitudes about video games and perceived linkages with youth assaults specifically. Results reflected continued lack of scholarly consensus on the issue of video game influences with only 15.3% of scholars endorsing the view that violent video games contribute to youth assaults. As hypothesized, older scholars endorsed more negative views of video games generally, although this appeared to be related to experience with games rather than age per se. Scholars with more negative attitudes toward youth themselves were also more negative about games. Criminologists and media scholars were more skeptical of violent video games contributing to youth assaults than were psychologists. These results are discussed in relation to Moral Panic Theory.     

A novel adaptive logic for dynamic adaptive streaming over HTTP

In this paper, we propose an estimation method that estimates the throughput of upcoming video segments based on variations in the network throughput observed during the download of previous video segments. Then, we propose a rate-adaptive algorithm for Hypertext Transfer Protocol (HTTP) streaming. The proposed algorithm selects the quality of the video based on the estimated throughput and playback buffer occupancy. The proposed method selects high-quality video segments, while minimizing video quality changes and the risk of playback interruption, improving user’s experience. We evaluate the algorithm for single- and multi-user environments and demonstrate that it performs remarkably well under varying network conditions. Furthermore, we determine that it efficiently utilizes network resources to achieve a high video rate; competing HTTP clients achieve equitable video rates. We also confirm that variations in the playback buffer size and segment duration do not affect the performance of the proposed algorithm.