以聚吡咯和纳米金为基质基于电化学交流阻抗测试的乙肝表面抗原免疫传感器研究

A novel simple immunosensing strategy for fabrication of hepatitis B surface antigen detection has been developed via electrochemical impedance spectroscopy （EIS） as a platform. At first, the conductive polymer polypyrrole （PPy） film was electrodeposited on a platinum electrode surface to adsorb the gold nanoparticles （nano-Au） via the opposite-charged adsorption technique, and then hepatitis B surface antibodies were adsorbed onto the surface of nano-Au. The modification procedure was characterized by EIS. Such spectroscopy is attributed to the concomitant conductivity changes of the polymerized pyrrole film and gold nanoparticles. The factors influencing the performance of resulting immunoelectrode were studied in detail. The linear range of the resulting immunoelectrode is from 2.6 to 153.6 ng.mL^-1 with a detection limit of 1.3 ng·mL^-1 at 3σ. In addition, the experiment results indicate that antibody immobilized on this way exhibits a good sensitivity, selectivity, high stability and a long-term maintenance of bioactivity, implying a great promising alternative approach for reagentless immunosensing analysis in the clinical diagnosis.     

环氧活化法制备免疫吸附剂及其对乙型肝炎表面抗原的吸附

分子采用环氧氯丙烷和1,4－二羟基正丁烷双缩水甘油醚活化交联壳聚糖树脂,经偶联抗－乙型肝炎表面抗原（HBsAg)单克隆抗体,制备树脂／抗体免疫吸附剂。含HBsAg的患者阳性血清的吸附实验结果表明,对HBsAg的吸附率可达44％,能使阳性血清转为阴性,通过对不同活化试剂制备的免疫吸附剂的活化过程和吸附性能的研究发现,活化试剂中的“手臂”结构有利于保持偶联抗的天然构象,使之具有较高的活性。     

肝生素治疗40例重型乙型病毒性肝炎的疗效初探

应用肝生素治疗各种重型乙型病毒性肝炎,结果表明,４０例重型乙型病毒肝炎患者经肝生素治疗,治疗组死亡１５例,病死率３７．５％,对照组死亡２６例,病死率６５．０％,两组比较有显著差异。肝生素在重型肝炎的早中期治疗效果特别理想,可认为肝生素是目前治疗重型病毒性肝炎比较理想的护肝药物。     

微量元素硒治疗肝病138例的探讨

用西维尔(硒酵母片)治疗了肝病138例，对其疗效进行了观察和探讨。结果表明，硒对各种肝炎、肝肿大者疗效显著；对已患肝癌或肝硬化者疗效不明显。     

应用于MCM-C/D的减薄抛光工艺

混合型多芯片组件(MCM-C/D)研制技术具有共烧陶瓷技术高密度多层互连集成和薄膜电路高精度和高可靠性等优点,是目前先进实用的混合集成技术.共烧多层基板的总厚度差(TTV)和表面粗糙度是影响共烧多层基板在多芯片组件(MCM)中应用的关键因素.选取低温共烧陶瓷基板,研究了减薄抛光工艺对基板的作用机理,结合实际加工要求选择...     

从提宗龙胆中分得的两个新的O-和C-山酮葡萄糖甙

从提宗龙胆地上部分分离出两个新的山酮葡萄糖甙3-0-β-D吡喃葡萄糖基-16-二羟基酮甙1和3-C-β-D吡喃糖基-1-羟基-7-甲氧基酮甙2.它们的结构用光谱方法(FABMS,'HNMR,DEPT和COSY)以及与相关化合物比较而得到确证.     

超薄化芯片

芯片的超薄化发展,正在逐步整合半导体材料加工设备及其相应工艺。简要概述了芯片超薄化发展之动态,并以超薄芯片的减薄加工为中心,综合研究了影响超薄芯片机械特性、表面质量的主要因素,讨论了超薄晶圆片的最新加工技术。     

Synthesis and anti-HBV activity of S-substituted 7-mercapto-4-methylcoumarin analogs

Twelve S-substituted 7-mercapto-4-methylcoumarin analogs were synthesized and evaluated for the inhibition to HBV in HepG2 2.2.1.5 cell. Among them, ten compounds exhibited potent inhibition to HBsAg and/or HBeAg with the IC50 values of sub μmol/L level. The ICso of anti-HBsAg activities of 5c and 5j reached 0.01 μmol/L respectively which were 16 fold more potent than that of 3TC. Compounds 3, 5e, 5g, 5h and 5i showed admirable inhibitory activity to both HBsAg and HBeAg. The bioassay results indicated the S-substituted 7-mercapto-4-methylcoumarin analogs merit attention as novel anti-HBV agents.     

肝炎患儿发锌,铜,铅,镉含量的分析

采用闰溶出法测定８２例肝炎患儿的头发中锌,铜,铅,镉含量。结果显示,大多数急,慢性肝炎患儿发锌含量降低;发铜增高者仅占１／４和１／８,肝慢性肝炎中发铜降低者红占１／３,为急性肝炎的３倍;     

Sesquiterpene Esters from Salvia roborowskii

Two new sesquiterpene esters,3β,6β,8α-triacetyl-4β,5α-epoxy-1-oxogermacr10(14)-ene(1)and 3β,6β,8α-triacetyl-4β,5α-epoxygermacr-1(10)-ene(2) were isolated from the whole plant of Salvia roborowskii Maxim.Their structures were elucidated by means of spectral data(2DNMR and HRMS).