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31.
可可碱与牛血清白蛋白作用光谱特性的研究 总被引:2,自引:0,他引:2
本文应用荧光光谱法研究了可可碱(TB)与牛血清白蛋白(BSA)相互作用的光谱特性。测定了18℃、30℃、40℃温度下的结合常数KA分别为1.68×10^4、1.58×10^4、1.45×10^4L/mol,结合位点数咒分别为1.04、1.03、1.03。实验结果表明:TB对BSA内源荧光的猝灭机理主要为静态猝灭;热力学参数探讨其相互作用机理,TB主要以静电力与BSA相互作用;研究了TB对BSA构象的影响,BSA的荧光主要源于色氨酸残基。同时研究了Cu^2+存在下TB与BSA的相互作用。 相似文献
32.
A nonlinear mathematical model is proposed to study the effect of tuberculosis on the spread of HIV infection in a logistically growing human population. The host population is divided into four sub classes of susceptibles, TB infectives, HIV infectives (with or without TB) and that of AIDS patients. The model exhibits four equilibria namely, a disease free, HIV free, TB free and an endemic equilibrium. The model has been studied qualitatively using stability theory of nonlinear differential equations and computer simulation. We have found a threshold parameter R0 which is if less than one, the disease free equilibrium is locally asymptotically stable otherwise for R0>1, at least one of the infections will be present in the population. It is shown that the positive endemic equilibrium is always locally stable but it may become globally stable under certain conditions showing that the disease becomes endemic. It is found that as the number of TB infectives decreases due to recovery, the number of HIV infectives also decreases and endemic equilibrium tends to TB free equilibrium. It is also observed that number of AIDS individuals decreases if TB is not associated with HIV infection. A numerical study of the model is also performed to investigate the influence of certain key parameters on the spread of the disease. 相似文献
33.
采用电感耦合等离子体原子发射光谱(ICP-AES)法对TB9钛合金中微量Si的测定进行了研究。总结了基体对较灵敏Si的8条分析线的光谱干扰,发现从Si 185.067nm到Si 288.158nm均有不同基体元素的干扰,对微量Si的测定影响很大。经研究对比,选用背景相对低且信噪比高的Si 288.158nm线,以试剂加定量V为空白来校正基体V的光谱叠加干扰,标准加入法测定。方法检出限0.04μg/mL,标准加入校准曲线的线性相关系数0.999 9。样品加标回收率为100%~105%,相对标准偏差(n=8)小于2.0%。方法简便可靠,可获得满意的分析结果。 相似文献
34.
建立了电感耦合等离子体原子发射光谱(ICP-AES)法快速测定TB6钛合金中Al、V的方法。对试样溶解酸浓度、元素分析谱线的选择、样品基体与待测元素间的干扰等因素进行了研究。结果表明,Al、V元素的加标回收率分别为97.5%~100%、98.4%~102%,相对标准偏差(RSD)分别为1.2%、0.45%,可用于TB6钛合金中Al、V含量的实际测定。 相似文献
35.
Diagnostic techniques for photonic materials based on Raman and Brillouin spectroscopies 总被引:1,自引:0,他引:1
M. Mattarelli S.Caponi A. Chiappini M. Montagna E. Moser F. Rossi C.Tosello C. Armellini A. Chiasera M. Ferrari Y. Jestin G. Nunzi Conti S. Pelli G.C. Righini 《光电子快报》2007,3(3):188-191
The elastic and vibrational properties of a material,bulk or planar waveguide,are studied by Brillouin and Raman spectroscopy to follow the process of nanocrystals growth in glass-ceramics. The nanoparticles cause the appearance,in the low fre-quency Raman spectrum,of characteristic peaks,whose position depends on the size of the crystals. At the same time,sharp crystal peaks,due to optical phonons,appear in the Raman spectra,allowing the determination of the nucleated phase,and a frequency shift of the Brillouin peaks is observed. 相似文献
36.
Md Amjad Beg Shivangi Obaid Afzal Md Sayeed Akhtar Abdulmalik S. A. Altamimi Afzal Hussain Md Ali Imam Mohammad Naiyaz Ahmad Sidharth Chopra Fareeda Athar 《Molecules (Basel, Switzerland)》2022,27(14)
The emergence of drug resistance and the limited number of approved antitubercular drugs prompted identification and development of new antitubercular compounds to cure Tuberculosis (TB). In this work, an attempt was made to identify potential natural compounds that target mycobacterial proteins. Three plant extracts (A. aspera, C. gigantea and C. procera) were investigated. The ethyl acetate fraction of the aerial part of A. aspera and the flower ash of C. gigantea were found to be effective against M. tuberculosis H37Rv. Furthermore, the GC-MS analysis of the plant fractions confirmed the presence of active compounds in the extracts. The Mycobacterium target proteins, i.e., available PDB dataset proteins and proteins classified in virulence, detoxification, and adaptation, were investigated. A total of ten target proteins were shortlisted for further study, identified as follows: BpoC, RipA, MazF4, RipD, TB15.3, VapC15, VapC20, VapC21, TB31.7, and MazF9. Molecular docking studies showed that β-amyrin interacted with most of these proteins and its highest binding affinity was observed with Mycobacterium Rv1636 (TB15.3) protein. The stability of the protein-ligand complex was assessed by molecular dynamic simulation, which confirmed that β-amyrin most firmly interacted with Rv1636 protein. Rv1636 is a universal stress protein, which regulates Mycobacterium growth in different stress conditions and, thus, targeting Rv1636 makes M. tuberculosis vulnerable to host-derived stress conditions. 相似文献
37.
38.
Staphylococcal enterotoxin B (SEB) was labeled with tetramethylrhodamine isothiocyanate (TRITC) and used as a probe for a competitive immunoassay. Labeling conditions such as solution pH and time were varied to observe the effect on the fluorescent product. It was found that solution pH of the labeling reaction had little effect on the fluorescence signal of the resulting products. However, labeling at pH 7.0 produced a probe that had a higher affinity for the antibody used in this study than the probes produced at pH 8.0 and 9.0. The fluorescent probes were used to perform a competitive assay for SEB in model skim milk samples. Detection limit was approximately 300 fg of SEB. Quantitation was achieved by curve fitting of fluorescent signals for bound/free probe versus log[SEB] with logarithmic functions. Accuracy in the model skim milk samples was acceptable for 3 and 5 nM SEB, but decreased considerably for a concentration of less than 1 nM SEB. The error was attributed to deviation in linearity in the standard curve at lower concentrations. Reproducibility for the analysis of both standard solutions used for the calibration curves and the model skim milk samples was excellent, with standard deviations of approximately 10% from data collected over a 3-week period. No cross-reactivity was found when the assay was tested with a 700 nM sample of staphylococcal enterotoxin A. Although competitive immunoassays are usually used for small molecules, such as therapeutic drugs, the results demonstrate that relatively large molecules (SEB, 27 kDa) can also be assayed with the technique. 相似文献
39.
Jianhua Huang a Lianzhong Zhang a b Suyuan Yu c a School of Physics Nankai University Tianjin China b State Key Laboratory of Loess Quaternary Geology Institute of Earth Environment Chinese Academy of Sciences Xi’an China c Institute of Nuclear Energy New Energy Technology Tsinghua University Beijing China 《中国颗粒学报》2011,9(1)
A representative human upper respiratory tract (URT) with idealized oral region and asymmetric tracheo-bronchial (TB) airway has been modeled, and laminar-to-turbulent airflow for typical inhalation modes as well as micro-particle transport and deposition has been simulated using CFX10.0 software from Ansys Inc. on a personal computer. The asymmetric TB airway could not be replaced by an extended straight tube as outlet of the oral region while investigating the tracheal airflow field and particle depositio... 相似文献
40.
A Solution to Optimizing Access Networks 总被引:1,自引:1,他引:0
1 ThePresentSituationofAccessNetworks Internationalcommunicationinformationtech nologiesarenowadaysinnovatinggreatly .Andthehigh techiswellingupcontinuously .Thetelecom municationnetworkisevolvinginthedirectionofthestratificationdiminutionandtheaugmento… 相似文献