Some preservation theorems in an intermediate logic

We prove some preservation theorems concerning inductive and model‐complete theories in the framework of semi‐classical logic introduced in [1]. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

甲基单胞菌GYJ3催化环氧丙烷的半连续合成

利用含有甲烷单加氢酶（MMO）的甲烷氧化细菌整催化丙烷环氧化制取环氧丙烷,其产物抑制和辅酶NADH消耗是反应难以连续进行的主要原因,采用甲烷再生NADH,研究了丙烯和甲烷共氧化和氧物氧丙烷积累对甲基单胞菌GYJ3的环氧化活性的影响,并考察了在具有产物连续收系统的两段式生物反应器中用细菌胞悬浮液半连续生产环氧丙烷的可行性,将再生和环氧化过程分开进行,以减小甲烷和丙烯的竞争抑制,通过连续抽提产物环氧丙烷,克服了产物抑制,该半连续过程分别在不同的温度下共进行了70h,产生环氧丙烷438μmol。     

‐symmetry and potentiel well. The simple well case

In this paper, we consider ‐symmetric perturbations of a semi‐classical self‐adjoint operator on the real axis in the case of a simple well potential Schrödinger operator. We assume that the potential is analytic and we show that the eigenvalues are left real by the perturbation.     

Investigation of a Biosensor Based on Phenylalanine Dehydrogenase Immobilized on a Polymer‐Blend Film for Phenylketonuria Diagnosis

We investigated a L ‐phenylalanine (L ‐phe) biosensor, functionalized through enzyme immobilization on a polymer‐blend film. The electron mediator 3,4‐dihydroxybenzaldehyde (3,4‐DHB) was employed at the electrode surface to improve direct oxidation of NADH to NAD⁺ and no additional reagents is required to be added to the sample solution. The bioactivated electrode was coated with a semi‐permeable cellulose acetate membrane in order to prevent dissolution of biofunctionalized polymer‐blend film. This constructed enzyme electrode is the first selective biosensor for phenylketonuria (PKU) detection. The sensitivity of the enzyme electrode was determined as 12.014 mA/M cm². The Michaelis–Menten and current responses as well as sensitivity of the electrode showed improved values than those of previous works. This selective biosensor presented an excellent electroanalytical response for L ‐phe, with a high steady‐state current being obtained after 20 s. The sensitivity of our biodevice is quite sufficient for the purpose of PKU detection because the reference range of clinical concern for L ‐phenylalanine concentration is C_{L ‐phe}>0.5 mM. This surface‐bioactivated enzyme electrode retained more than 80 % of its electrocatalytic activity after 16 days.     

Open microfluidic gel electrophoresis: Rapid and low cost separation and analysis of DNA at the nanoliter scale

Gel electrophoresis is one of the most applied and standardized tools for separation and analysis of macromolecules and their fragments in academic research and in industry. In this work we present a novel approach for conducting on‐demand electrophoretic separations of DNA molecules in open microfluidic (OM) systems on planar polymer substrates. The approach combines advantages of slab gel, capillary‐ and chip‐based methods offering low consumable costs (<0.1$) circumventing cost‐intensive microfluidic chip fabrication, short process times (5 min per analysis) and high sensitivity (4 ng/μL dsDNA) combined with reasonable resolution (17 bases). The open microfluidic separation system comprises two opposing reservoirs of 2–4 μL in volume, a semi‐contact written gel line acting as separation channel interconnecting the reservoirs and sample injected into the line via non‐contact droplet dispensing and thus enabling the precise control of the injection plug and sample concentration. Evaporation is prevented by covering aqueous structures with PCR‐grade mineral oil while maintaining surface temperature at 15°C. The liquid gel line exhibits a semi‐circular cross section of adaptable width (∼200–600 μm) and height (∼30–80 μm) as well as a typical length of 15–55 mm. Layout of such liquid structures is adaptable on‐demand not requiring time consuming and repetitive fabrication steps. The approach was successfully demonstrated by the separation of a standard label‐free DNA ladder (100–1000 bp) at 100 V/cm via in‐line staining and laser induced fluorescent end‐point detection using an automated prototype.     

带机器准备时间的平行机ordinal排序及近似算法

本文研究带机器准备时间的m台平行机ordinal在线排序问题。讨论了在极小化最大机器完工时间和极小化最大工件完工时间两种目标下的不同下界和相应的在线近似算法。对第一个目标，我们得到了3/2的下界和最坏情况界为2-1/m的近似算法。对第二个目标，我们得到了最坏情况为m的最好近似算法。我们还对一些特殊情况进行了分析。     

连续波激光器腔内倍频晶体半解析分析方法的研究

连续波激光器系统腔内倍频非线性光学晶体吸收基频光辐射产生谐波的同时,也会在晶体内部产生非均匀温升,引起折射率变化,破坏位相匹配条件,影响激光器谐波转换效率.解决问题的关键是准确得出晶体内部温度场.由于激光器中激光模式和边界条件的复杂性,无法得到温度场的解析解.利用半解析热分析方法得出了晶体工作在TEM00模式下产生的温度场计算公式,分析了对温度场分布的各种影响.所得结果对连续波腔内倍频激光系统设计将起到指导作用.     

Semi cover-avoiding properties of finite groups

In this paper, we characterize the nilpotency and supersolvability of a finite group G by assuming some subgroups of prime power order have the semi cover-avoiding property in G. Some earlier results are generalized.     

关于M/M/1动态排队系统解的半稳定性

本文讨论了两类 M/M/1 动态系统的数学模型 ,利用常微分方程所描述的 M/M/1 系统的结果证明了较复杂的偏微分方程所描述的 M/M/1 系统的一些性质 ,该方法简化了已有结果     

半参数EV模型参数的二阶段估计

本文综合核函数法 ,最小二乘法 ,利用二阶段估计的方法求出了 EV模型中参数的估计量 ,并研究了它的强相合性以及渐近正态性 .