Lipid analysis by thin-layer chromatography--a review of the current state

High-performance thin-layer chromatography (HPTLC) is a widely used, fast and relatively inexpensive method of separating complex mixtures. It is particularly useful for smaller, apolar compounds and offers some advantages over HPLC. This review gives an overview about the special features as well as the problems that have to be considered upon the HPTLC analysis of lipids. The term "lipids" is used here in a broad sense and comprises fatty acids and their derivatives as well as substances related biosynthetically or functionally to these compounds. After a short introduction regarding the stationary phases and the methods how lipids can be visualized on an HPTLC plate, the individual lipid classes will be discussed and the most suitable solvent systems for their separation indicated. The focus will be on lipids that are most abundant in biological systems, i.e. cholesterol and its derivates, glycerides, sphingo- and glycolipids as well as phospholipids. Finally, a nowadays very important topic, the combination between HPTLC and mass spectrometric (MS) detection methods will be discussed. It will be shown that this is a very powerful method to investigate the identities of the HPTLC spots in more detail than by the use of common staining methods. Future aspects of HPTLC in the lipid field will be also discussed.     

SM2高速双域 Montgomery 模乘的硬件设计

作为由国家密码管理局公布的SM2椭圆曲线公钥密码算法的核心运算,模乘的实现好坏直接决定着整个密码芯片性能的优劣．Montgomery模乘算法是目前最高效也是应用最为广泛的一种模乘算法．本文基于Mont-gomery模乘算法,设计了一种高速,且支持双域（GF（p）素数域和GF（2m ）二进制域）的Montgomery模乘器．提出了新的实现结构,以及一种新型的W allace树乘法单元．通过对模块合理的安排和复用,本设计极大的缩小了时间消耗与硬件需求,节省了大量的资源．实现256位双域模乘仅需0．34μs ．     

上海移动iPhone4S手机无法发送短信投诉故障分析

iPone4S属于中国移动的高端用户,对于iPhone4S用户在使用中出现的各种应用问题和投诉,中国移动针对用户的投诉问题快速响应并进行深入信令分析,对用户终端问题、无线网络问题、核心网问题、用户漫游权限问题进行具体定位。对于各省维护人员处理漫游收发短信失败投诉具有很好的指导意义,充分发挥了信令监测系统在投诉处理方面的快速分析定位问题的作用,提升了用户对移动网络的满意度。     

参麦注射液联合川芎嗪对脓毒症小鼠的肾保护作用

目的：探讨参麦注射液联合川芎嚷对盲肠结扎穿孔术（cecalligatignandptmcture．CLP）所致脓毒症小鼠的肾保护作用。方法：给小鼠行盲肠结扎穿孔术复制脓毒症模型,分别用川芎嗪注射液（55mg／kg）,参麦注射液（500mg/kg）或参麦注射液（500mg/kg）联合川芎嗪注射液（55mg/kg）进行干预。造模后24h检测肾损伤程度和炎症反应强度,72h统计小鼠存活翠。结果：川芎嗪注射液、参麦注射液以壁参麦注射液联畲川芎嗪可使脓毒症小鼠的存活率提高,血清尿素氮（bloodu Ieanitrogen,BUN）和肌酐（creatinineCr）水平降低,肾损伤减轻;同时使血清白介素-6（Interleukin-6,IL-6）和肿瘤坏死因子（tumornecmsisfactor,TNF-α）水平下降,其中以参麦注射液联合川芎嗪的效果最佳。结论：参麦注射液联合川芎嗪较单一给药对脓毒症小鼠具有更好的肾保护作用。     

SM2965微控制器及其ISP应用技术

文中分析了SynCMOS公司的在线可编程（ISP）微控制器SM2965的性能特点，存贮器结构及特殊功能寄存器，介绍了SM2965的ISP功能和使用方法。     

基于FPGA的1553B总线接口技术研究与实现

为了满足某型机载显示器与机上其他设备的复杂任务和可靠的数据通信,文章提出了一种基于FPGA控制协议芯片SM61864G3实现1553B总线远程终端通信的设计方法。该设计将整个系统的数据处理与接口控制逻辑集成在FPGA内实现,保障系统实时的可确定性。测试结果表明:该通用接口实时性及可靠性高,实际应用效果良好。     

空间调制系统低复杂度的天线选择算法

空间调制(Spatial Modulation,SM)是一种特殊的多天线传输技术,利用发送天线索引和发送的符号共同传递信息.为了获得发送分集增益,人们将天线选择技术应用到SM系统中,提高SM系统解调性能.在天线选择技术中,最大-最小欧式距离(Euclidean Distance Antenna Selection,EDAS)准则应用较为广泛,但是它的全搜索求解方法复杂度高,限制了其应用.为此,本文利用空间调制系统和调制符号本身的特性,从2维量化解调的视角出发,给出了两种低复杂度的最优天线选择算法,并通过计算机仿真和复杂度分析,表明了该算法的有效性和最优性.     

Mutual information power analysis attack of HMAC based on SM3

A novel method of mutual information power analysis attack was proposed.The method was built on the basis of the basic principle of power analysis and the basic theory of information.For the purpose of attacking the key,the mutual in-formation values was computed using two values between the mediate variable with the power traces.An experiment was im-plemented on the algorithm of HMAC based on SM3 using this method.The experimental results show the proposed attack method is effective because the initial value of state variable can be successfully retrieved to compute the real true key.     

Comparison of different HPLC systems for analysis of galantamine and lycorine in various species of Amaryllidaceae family

Retention parameters of galantamine and lycorine standards were determined on different columns, i.e., octadecyl silica, SM C18, and strong cation-exchange (SCX) columns with different aqueous mobile phases. Retention of alkaloids was investigated on C18, SM C18 columns with mobile phase containing 5% MeCN, 20% acetate buffer at pH 3.5, and 0.025 ML^?1 diethylamine (DEA), and on SCX column with mobile phase containing 8% MeCN and phosphate buffer at pH 2.5. Better results were also obtained in ion-exchange chromatographic system. On the basis of results obtained in different chromatographic systems, simple, rapid, and sensitive high-performance liquid chromatography methods were developed for determining lycorine and galantamine in plant extracts from various species belonging to Amaryllidaceae family. Extracts were prepared from various parts of plants collected at different times of the growing season.     

Flow-through immunomagnetic separation system for waterborne pathogen isolation and detection: Application to Giardia and Cryptosporidium cell isolation

Simultaneous sample washing and concentration of two waterborne pathogen samples were demonstrated using a rotational magnetic system under continuous flow conditions. The rotation of periodically arranged small permanent magnets close to a fluidic channel carrying magnetic particle suspension allows the trapping and release of particles along the fluidic channel in a periodic manner. Each trapping and release event resembles one washing cycle.The performance of the magnetic separation system (MSS) was evaluated in order to test its functionality to isolate magnetic-labelled protozoan cells from filtered, concentrated tap water, secondary effluent water, and purified water. Experimental protocols described in US Environmental Protection Agency method 1623 which rely on the use of a magnetic particle concentrator, were applied to test and compare our continuous flow cell separation system to the standard magnetic bead-based isolation instruments. The recovery efficiencies for Giardia cysts using the magnetic tube holder and our magnetic separation system were 90.5% and 90.1%, respectively, from a tap water matrix and about 31% and 18.5%, respectively, from a spiked secondary effluent matrix. The recovery efficiencies for Cryptosporidium cells using the magnetic tube holder and our magnetic separation system were 90% and 83.3%, respectively, from a tap water matrix and about 38% and 36%, respectively, from a spiked secondary effluent matrix. Recoveries from all matrices with the continuous flow system were typically higher in glass tubing conduits than in molded plastic conduits.