在Final Cut Pro中模拟切换台功能

在拍摄一些中小型的文艺晚会时,可能会用到2-4台摄像机进行拍摄,以满足不同景别和角度的需要。如果没有视频切换台现场切换,在后期非线性编辑时间线上拖动多轨的视频做快速切换的剪辑效果,不但会花费很长的时间,而且剪辑起来相当的繁琐复杂。如果用苹果非编软件中的多镜头编辑功能,就可以轻松的解决问题,本文着重讲述如何利用Final Cut Pro软件中的多镜头编辑功能来模拟实现对多路视频进行切换剪辑。     

Pro/E行为建模技术在天线配重设计中的应用

针对天线配重设计中存在的问题，利用先进的设计软件Pro／E中的高级模块——“行为建模”技术对配重设计进行了优化，使配重设计更加简单、准确、高效。     

Pro Tools光纤网络系统设计分析

分析了Pro Tools光纤网络系统的构成、特点,对系统工作流程进行了简要说明,特别强调了项目中重点考虑的几点问题,最后从网络技术核心、按需要组织的应用流程等方面设计了此网络系统。     

Sensitive phosphoprotein detection in SDS‐PAGE via Anthracene Chrome Red A stain

Protein phosphorylation, one of the most important post‐translational modifications, plays critical roles in many biological processes. Thus, it is necessary to precisely detect, identify and understand the phosphoproteins from protein mixture for the study of cell biology. We introduce a sensitive and specific detection method for phosphoproteins in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Anthracene Chrome Red A (ACRA) combined with the trivalent metal ion (Al³⁺) is converted to fluorescent complex and the fluorescence is sharply increased by a change of pH environment. Phosphoproteins and non‐phosphoproteins can be easily distinguished by the fluorescence quenching due to the structural change of ACRA‐Al³⁺‐phosphoprotein complex, unlike non‐phosphoprotein complex. The method using ACRA is a negative staining based on the fluorescence quenching and has a high sensitivity comparable to Pro‐Q Diamond stain. ACRA stain can detect 1–2 ng of α‐casein and β‐casein, 8–16 ng of ovalbumin (OVA) and κ‐casein within 130 min. Moreover, the ACRA stain showed similar linear dynamic ranges and RSD to Pro‐Q stain. The linear dynamic ranges of ACRA and the values of correlation coefficient were for OVA (8–500 ng, correlation coefficient r = 0.999), α‐casein (4–500 ng, r  = 0.992), β‐casein (4–500 ng, r  = 0.996), and κ‐casein (8–500 ng, 0.998), respectively. On the other hand, the values of the relative standard deviations (RSD) ranged from 2.33 to 3.56% for ACRA. The method is sensitive, specific, simple, rapid and compatible with total protein stain such as SYPRO Ruby stain. Therefore, ACRA stain can be an advanced method for phosphoprotein detection in gels.     

Xbee Pro模块在无线供热计量系统中的应用

针对我国供热收费改革对热计量系统迫切需求的现状,本文设计了一种基于无线自组网络的热量远程传输系统。网络系统的节点采用MaxStream 公司的Xbee Pro模块和TI 公司的低功耗控制芯片MSP430FW427,并采用无线网状网的组网方式,最终实现了低功耗、低复杂度的传输系统。使用户和供暖公司能够共同监测热量表的运行情况,也实现热计量系统管理、监控的信息化、自动化。     

5．1多轨录制系统设备配置及功能应用

重点介绍了陕西人民广播电台建设的数字化5．1多轨录制系统,包括ICON系统的硬件组成,核心控制设备D—Control及其应用软件Pro Tools的功能等,最后结合实际应用对使用中总结出的经验和技巧进行了介绍。     

Alternative visualization of SDS‐PAGE separated phosphoproteins by alizarin red S‐aluminum (III)‐appended complex

A novel fluorescence detection system using a chemosensor for phosphoprotein in gel electrophoretic analysis has been developed. The system employed the alizarin red S‐aluminum (III)‐appended complex as a fluorescent staining dye to perform the convenient and selective detection of phosphorylated proteins and total proteins in SDS‐PAGE, respectively. Therefore, a full and selective map of proteins can be achieved in the same process without resorting to other compatible detection methods. As low as 62.5 ng of α‐ (seven or eight phosphates) and β‐casein (five phosphates), 125 ng of ovalbumin (two phosphates), and κ‐casein (one phosphate) can be detected in approximately 135 min, with the linear responses of rigorous quantitation of changes over a 125–4000 ng range. As a result, alizarin red S‐aluminum (III) stain may provide a new choice for selective, economic, and convenient visualization of phosphoproteins.     

Asymmetric Aldol Reactions in Caprolactam?Quaternary Ammonium Salt Coordination Ionic Liquid Catalyzed by L‐Pro‐L‐Trp

A direct asymmetric aldol reaction of aldehydes and acetone catalyzed by L ‐Pro‐L ‐Trp was performed in β‐caprolactam? quaternary ammonium salt coordination ionic liquid media in the presence of N‐methylmorpholine (NMM) in high yield and with good enantioselectivity. The approach has the advantages of simple product isolation, and reusable catalyst and coordination ion liquids.     

基于信息化思维的榆林小曲文化保护路径创新研究

榆林小曲是我国第一批非物质文化遗产,却存在后继无人、年龄结构老化等问题,榆林小曲面临着消亡的危机。针对这一现象,本文探究了基于信息化思维的榆林小曲文化保护路径的创新研究,该保户工作是在Cool Edit Pro音频制作软件平台上实现的。     

无人机飞行试验调试装置设计

设计了一种无人机飞行试验调试装置.利用DSP、XBee Pro(无线模块)和上位机等来实现上行数据的发送和下行数据的显示及存储.分析了调试装置各个模块的功能,对该装置进行了硬件设计,应用CCS和VB软件为开发平台,对DSP串口通讯和调参软件等进行了相应的程序设计.对系统进行了调试,结果表明,调试装置很好地实现了系统所要...