补体C1q压电免疫传感器的研究

研制了一种可重复使用的压电免疫传感器用于检测补体C1q.比较了聚乙烯亚胺粘附、戊二醛交联法,物理吸附法以及蛋白A法三种固定化抗体蛋白的方法.使用蛋白A法,补体C1q在所测浓度范围内有良好的线性关系.使用过的晶片用0.1 mol/L柠檬酸盐缓冲溶液(pH 3.0)解吸,可重复使用4次.     

Lead zirconate titanate cantilever for noncontact atomic force microscopy

Noncontact atomic force microscopy with frequency modulation detection is a promising technique for surface observation with true atomic resolution. The piezoelectric material itself can be an actuator and sensor of the oscillating probe simultaneously, without the need for additional electro-mechanical transducers or other measurement systems. A vertical resolution of 0.01 nm rms has been achieved using a microfabricated cantilever with lead zirconate titanate thin film in noncontact mode frequency modulation detection. The cantilever also has a sharpened pyramidal stylus with a radius of about 10 nm for noncontact atomic force microscopy.     

Opening and contact zones of an interface crack in a piezoelectric bimaterial under combined compressive-shear loading

A plane problem for a tunnel electrically permeable interface crack between two semi-infinite piezoelectric spaces is studied. A remote mechanical and electrical loading is applied. Elastic displacements and potential jumps as well as stresses and electrical displacement along the interface are presented using a sectionally holomorphic vector function. It is assumed that the interface crack includes zones of crack opening and frictionless contact. The problem is reduced to a combined Dirichlet–Riemann boundary value problem which is solved analytically. From the obtained solution, simple analytical expressions are derived for all mechanical and electrical characteristics at the interface. A quite simple transcendental equation, which determines the point of separation of open and close sections of the crack, is found. For the analysis of the obtained results, the main attention is devoted to the case of compressive-shear loading. The analytical analysis and numerical results show that, even if the applied normal stress is compressive, a certain crack opening zone exists for all considered loading values provided the shear field is present. It is found that the shear stress intensity factor at the closed crack tip and the energy release rates at the both crack tips depend very slightly on the magnitude of compressive loading.     

Piezoelectric scattering limited mobility characteristics of a degenerate surface layer in compound semiconductors at low lattice temperatures

The theory is developed for piezoelectric scattering rate of carriers in a degenerate surface layer under the condition of low temperature when the approximations of the well-known traditional theory are not valid. The scattering rates thus obtained are then used to estimate the zero-field mobility characteristics for the surface layers under similar condition of low temperature. The results for the surface layers in GaAs and ZnO show that when one takes into account either the degeneracy of the carrier ensemble or the finite energy of the phonons or both, the energy dependence of the scattering rates changes significantly from what follows for a non-degenerate ensemble or from the traditional theory, where one makes use of the high-temperature approximation and thus assumes equipartition law for the phonon distribution, and neglects the phonon energy in the energy balance equation of the electron–phonon system. It is observed that the zero-field mobility characteristics that follow from these scattering rates are interesting in that they are quite different from what turns out either for a non-degenerate ensemble or in the high-temperature approximations.     

Lead-free KNLNT piezoelectric ceramics for high-frequency ultrasonic transducer application

This paper presents the latest development of a lead-free piezoelectric ceramic and its application to transducers suitable for high-frequency ultrasonic imaging. A lead-free piezoelectric ceramic with formula of (K_0.5Na_0.5)_0.97Li_0.03(Nb_0.9 Ta_0.1)O₃ (abbreviated as KNLNT-0.03/0.10) was fabricated and characterized. The material was found to have a clamped dielectric constant ε₃₃^S/ε₀ = 890, piezoelectric coefficient d₃₃ = 245 pC/N, electromechanical coupling factor k_t = 0.42 and Curie temperature T_c > 300 °C. High-frequency (40 MHz) ultrasound transducers were successfully fabricated with the lead-free material. A representative lead-free transducer had a bandwidth of 45%, two-way insertion loss of -18 dB. This performance is comparable to reported performances of popular lead-based transducers. The comparison results suggest that the lead-free piezoelectric material may serve as an alternative to lead-based piezoelectric materials for high-frequency ultrasonic transducer applications.     

基于巯基和聚电解质双层自组装膜的压电免疫传感器检测小鼠IgG气溶胶

本文结合自组装单分子层膜(SAMs)和聚电解质静电吸附组装技术,提出了一种新的用于气相压电免疫检测的生物分子固定化方法,研制了一种用于检测小鼠IgG抗体的压电免疫传感器。首先在石英晶片的金电极表面自组装了一层L-胱氨酸SAMs,再在膜上组装带相反电荷的海藻酸钠,最后通过调节pH值定向固定羊抗鼠纯化抗体,优化了固定条件。通过超声雾化法产生的小鼠IgG气溶胶,研制成了直接气相检测小鼠IgG的压电免疫系统。结果表明,该方法对所固定的生物分子活性影响较小,传感器对小鼠IgG的响应快,灵敏度高,在0.14~6μg.μL-1范围内具有良好的线性关系,精密度好,再生方便。     

塑料光纤相位调制灵敏度的研究

研究了压电涂层PMMA纤芯塑料光纤中的相位调制.射频信号通过对光纤外层的压电涂层作用,使纤芯中产生应变,从而对光纤中的光信号进行相位调制.数值计算结果表明,与传统的石英光纤相比,PMMA塑料光纤的相位调制的灵敏度要高数十倍以上.     

A piezoelectric immunosensor for the determination of pesticide residues and metabolites in fruit juices

A quartz crystal microbalance (QCM) immunosensor was developed for the determination of the insecticide carbaryl and 3,5,6-trichloro-2-pyridinol (TCP), the main metabolite of the insecticide chlorpyrifos and of the herbicide triclopyr. The detection was based on a competitive conjugate-immobilized immunoassay format using monoclonal antibodies (MAbs). Hapten conjugates were covalently immobilized, via thioctic acid self-assembled monolayer (SAM), onto the gold electrode sensitive surface of the quartz crystal. This covalent immobilization allowed the reusability of the modified electrode surface for at least one hundred and fifty assays without significant loss of sensitivity. The piezoimmunosensor showed detection limits (analyte concentrations producing 10% inhibition of the maximum signal) of 11 and 7 μg l⁻¹ for carbaryl and TCP, respectively. The sensitivity attained (I₅₀ value) was around 30 μg l⁻¹ for both compounds. Linear working ranges were 15-53 μg l⁻¹ for carbaryl and 13-83 μg l⁻¹ for TCP. Each complete assay cycle took 20 min. The good sensitivity, specificity, and reusability achieved, together with the short response time, allowed the application of this immunosensor to the determination of carbaryl and TCP in fruits and vegetables at European regulatory levels, with high precision and accuracy.     

一类弹性系统的精确L2-能控性

该文研究带有压电驱动器的Rayleigh梁系统的精确能控性.先用算子半群方法和提升结果[9]建立了Rayleigh梁方程解的正则性;再用Hilbert唯一性方法结合Diophantine逼近理论的某些结果得到了系统的精确L2-能控性.     

A three-layer structure model for the effect of a soft middle layer on Love waves propagating in layered piezoelectric systems

A three-layer structure model is proposed for investigating the effect of a soft elastic middle layer on the propagation behavior of Love waves in piezoelectric layered systems, with "soft" implying that the bulk-shear-wave velocity of the middle layer is smaller than that of the upper sensitive layer. Dispersion equations are obtained for unelectroded and traction-free upper surfaces which, in the limit, can be reduced to those for classical Love waves. Systematic parametric studies are subsequently carried out to quantify the effects of the soft middle layer upon Love wave propagation, including its thickness, mass density, dielectric constant and elastic coefficient. It is demonstrated that whilst the thickness and elastic coefficient of the middle layer affect significantly Love wave propagation, its mass density and dielectric constant have negligible influence. On condition that both the thickness and elastic coefficient of the middle layer are vanishingly small so that it degenerates into an imperfectly bonded interface, the three-layer model is also employed to investigate the influence of imperfect interfaces on Love waves propagating in piezoelectric layer/elastic substrate systems. Upon comparing with the predictions obtained by employing the traditional shear-lag model, the present three-layer structure model is found to be more accurate as it avoids the unrealistic displacement discontinuity across imperfectly bonded interfaces assumed by the shearlag model, especially for long waves when the piezoelectric layer is relatively thin.