半抗原的设计、修饰及人工抗原的制备

制备具有良好免疫原性的人工抗原是建立测定小分子化合物的免疫分析方法的最关键步骤.本文对半抗原的设计、修饰(修饰位点的选择、修饰物的制备、连接臂的选择)、载体的选择、半抗原与载体的偶联(偶联方式、偶联条件、最佳偶联率)、人工抗原的纯化与鉴定等方面作了详细的介绍.     

MCSS-based predictions of RNA binding sites

The diversity of RNA tertiary structures provides the basis for specific recognition by proteins or small molecules. To investigate the structural basis and the energetics which control RNA-ligand interactions, favorable RNA binding sites are identified using the MCSS method, which has been employed previously only for protein receptors. Two different RNAs for which the structures have been determined by NMR spectroscopy were examined: two structures of the TAR RNA which contains an arginine binding site, and the structure of the 16S rRNA which contains an aminoglycoside binding site (paromomycin). In accord with the MCSS methodology, the functional groups representing the entire ligand or only part of it (one residue in the case of the aminoglycosides) are first replicated and distributed with random positions and orientations around the target and then energy minimized in the force field of the target RNA. The Coulombic term and the dielectric constant of the force field are adjusted to approximate the effects of solvent-screening and counterions. Optimal force field parameters are determined to reproduce the binding mode of arginine to the TAR RNA. The more favorable binding sites for each residue of the aminoglycoside ligands are then calculated and compared with the binding sites observed experimentally. The predictability of the method is evaluated and refinements are proposed to improve its accuracy. Received: 24 April 1998 / Accepted: 4 August 1998 / Published online: 7 December 1998     

The development and application of guidance on equipment qualification of analytical instruments

 This paper describes the development of guidance for the equipment qualification (EQ) of analytical instruments. EQ is a formal process that provides documented evidence that an instrument is fit for its intended purpose and kept in a state of maintenance and calibration consistent with its use.     

传统应用集成系统的数据转换框架研究

文章在对数据转换需求模型进行分析的基础上,通过设计抽象,给出了一个易于扩展和复用的数据转换框架模型,并且适用于以面向过程语言为基础的、传统应用集成系统数据转换机制的设计与实现.     

NOrth-MDPSK系统的最佳检测算法

在多进制正交扩频MDPSK复合调制系统中,现有的检测算法先后独立地进行正交扩频比特与差分相位比特的检测,这使得差分相位比特检测性能与正交检测的性能息息相关,限制了整个系统的误比特性能。针对这一情况,本文基于最大后验概率(MAP)准则,提出了NOrth-MDPSK最佳检测算法。该算法根据差分相位检测的特点,充分利用前后两个符号周期内所有的正交相关值,联合检测当前符号的正交扩频比特和差分比特。仿真结果表明,本文所提算法能够有效地缓解正交检测的性能对系统性能的限制。     

一种高速USB设备控制器IP核的设计与实现

文章首先分析USB2．0协议，然后介绍基于该协议的一种设备控制器IP核设计实现，着重分析了设计中的几个问题并提出相应的解决方案，最后简单介绍了IP核的系统验证。     

风力发电机叶轮的数值优化设计法

１引言目前，在风力机叶轮的气动设计方面，还没有其系统的设计模型和方法，只有一些针对某一方面的模型，这些模型还无法规纳成一套可靠的系统的设计模型［１］，Ｇｏｕｒｉｅｒｅｓ在他的风力机设计理论的书中也只能介绍几种简化的有关设计模型［２］。所用的设计方法主要还是以经验设计为主，不是全面系统地考虑叶轮的各种影响因素，如Ｈａｂａｌｉ［３］在他的设计工作中主要考虑了叶片翼型对叶轮运行的气动作用，ＶＯｕｔｓｉｎａｓ［４］只是对偏航工况下叶轮的气动特性及操作进行了分析研究，文献［５］也仅对叶轮功率等气动特性进行…     

Optimum volume profile in axi-symmetric Stokes flow

In this paper, the problem of optimum volume profile under constant drag constraint when uniform stream is parallel to the axis of symmetry and flow is governed by Stokes equations has been tackled. Here, we take up a class of bodies to be of given maximum cross-section with fore and aft symmetry about this section. The possible shape under the stationary value drag has been obtained by making use of method of extremals [Fox C (1950) An introduction to the calculus of variations. Oxford University Press, Oxford; Elsgolc EC (1962) Differential equations and the calculus of variations. Pergamon Press; Sagan H (1969) Introduction to the calculus of Variations. MacGrawhill]. It has been found that the body profile possesses conical front and rear ends. The numerical value of the optimized volume has also been calculated for the profile and compared with some known values.