An Experimental Design Approach to Quantitative Expression for Quality Control of a Multicomponent Antidiabetic Formulation by the HILIC Method

A rapid and reproducible hydrophilic liquid chromatography (HILIC) process was established for concomitant determination of remogliflozin etabonate (RE), vildagliptin (VD), and metformin (MF) in a formulation. A face-centered central composite experimental design was employed to optimize and predict the chromatographic condition by statistically studying the surface response model and design space with desirability close to one. A HILIC column with a simple mobile phase of acetonitrile (65% v/v) and 20 mM phosphate buffer (35% v/v, pH 6, controlled with orthophosphoric acid) was used to separate RE, VD, and MF. RE, VD, and MF were separated in 3.6 min using an isocratic mode mobile phase flow at a flow rate of 1.4 mL at room temperature, and the analytes were examined by recording the absorption at 210 nm. The developed HILIC method was thoroughly validated for all parameters recommended by ICH, and linearity was observed in the ranges 20–150 µg/mL, 10–75 µg/mL, and 50–750 µg/mL for RE, VD, and MF, respectively, along with excellent regression coefficients (r² > 0.999). The calculated percentage relative deviation and relative error ascertained the precision and accuracy of the method. The selectivity and accuracy were further confirmed by the high percentage recovery of added standard drugs to the formulation using the standard addition technique. The robustness of the HILIC processes was confirmed by developing a half-normal probability plot and Pareto chart, as the slight variation of a single factor had no significant influence on the assay outcomes. Utilization of the optimized HILIC procedure for concurrent quantification of RE, VD, and MF in solid dosage forms showed accurate and reproducible results. Hence, the fast HILIC method can be regularly employed for the quality assurance of pharmaceutical preparations comprising RE, VD, and MF.     

一种沟槽-场限环复合终端结构的设计

为了改善硅功率器件击穿电压性能以及改善IGBT电流的流动方向,提出了一种沟槽-场限环复合终端结构。分别在主结处引入浮空多晶硅沟槽,在场限环的左侧引入带介质的沟槽,沟槽右侧与场限环左侧横向扩展界面刚好交接。结果表明,这一结构改善了IGBT主结电流丝分布,将一部分电流路径改为纵向流动,改变了碰撞电离路径,在提高主结电势的同时也提高器件终端结构的可靠性;带介质槽的场限环结构进一步缩短了终端长度,其横纵耗尽比为3.79,较传统设计的场限环结构横纵耗尽比减少了1.48%,硅片利用率提高,进而减小芯片面积,节约制造成本。此方法在场限环终端设计中非常有效。     

Simultaneous Estimation of Escitalopram and Clonazepam in Tablet Dosage Forms Using HPLC-DAD Method and Optimization of Chromatographic Conditions by Box-Behnken Design

The study aimed to develop a new reverse-phase high-performance liquid chromatography (RP-HPLC) method with diode array detection (DAD) detection for simultaneous estimation of escitalopram (EST) and clonazepam (CZP) in tablet dosage forms with a quality by design (QbD) approach. The chromatographic conditions were optimized by Box-Behnken design (BBD) and developed method was validated for the linearity, system suitability, accuracy, precision, robustness, sensitivity, and solution stability according to International Council for Harmonization (ICH) guidelines. EST and CZP standard drugs peaks were separated at retention times of 2.668 and 5.046 min by C-18 column with dimension of 4.6 × 100 mm length and particle size packing 2.5 µm. The mobile phase was methanol: 0.1% orthophosphoric acid (OPA) (25:75, v/v), with a flow rate of 0.7 mL/min at temperature of 26 °C. The sample volume injected was 20 µL and peaks were detected at 239 nm. Using the standard calibration curve, the % assay of marketed tablet was founded 98.89 and 98.76 for EST and CZP, respectively. The proposed RP-HPLC method was able to detect EST and CZP in the presence of their degradation products, indicating the stability-indicating property of the developed RP-HPLC method. The validation parameter’s results in terms of linearity, system suitability, accuracy, precision, robustness, sensitivity, and solution stability were in an acceptable range as per the ICH guidelines. The newly developed RP-HPLC method with QbD application is simple, accurate, time-saving, and economic.     

一种基于模式的集中控制软件架构的设计

为适应各种控制管理类系统的发展,传统的软件设计开发方式已不能满足需求。为此,研究并设计了一种基于模式的集中控制系统软件架构。实际应用结果表明,该软件架构具有较强的可复用性和扩展性。     

一种高性能太阳敏感器复合光学系统设计

以夫朗禾费衍射理论为基础,结合微透镜,设计了一种高性能的数字式太阳敏感器复合光学系统。分析了光学系统的组成,建立了光学系统的数学模型,设计了光学系统的焦距、孔径和微透镜参量,并进行了成像的数值仿真。仿真结果表明,与基于掩模的光学系统相比,这种新型的复合光学系统成像光斑能量分布集中,保证计算光斑中心位置的高精度,使敏感器具有大视场和高精度的特点。     

基于DVI-DVI处理器的两种亮度逐点算法分析

基于DVI-DVI处理器的亮度逐点校正算法有两种,即算法1和算法2.针对采用两种算法后在亮度偏差量以及数字电路设计方面的差异性进行分析.首先,通过色度学空间转换理论获得校正算法1和校正算法2的模型;然后,分别对采用两种算法引起的亮度偏差量进行对比,通过计算得到算法2和算法1的亮度偏差量比例系数kr;最后,对实现两种算法数字电路应用的资源进行对比.通过两种算法在这两方面的对比得出:算法2更适合应用于DVI-DVI处理器.     

Balancing CapEx reduction and network stability with stable routing–virtual topology capacity adjustment (SR–VTCA)

This paper investigates the merits of the SR–VTCA (stable routing–virtual topology capacity adjustment) approach as a mechanism to find a beneficial trade-off between network stability and reduction in capital expenditures (CapEx). These are two main objectives for the entities that own the optical infrastructure, such as network operators (NOs), and those also acting as Internet service providers (ISPs). The SR–VTCA scheme is a novel approach to adapt transparent optical networks to time-varying traffic by adjusting the number of lightpaths between node pairs, while keeping the IP routing unchanged. Lightpath bundling (LB) and anycast (AS) switching are combined in SR–VTCA operation to advertise lightpath additions/removals to the IP layer as mere adjustments (increments or decrements) in the capacity, allowing to keep the IP routing stable, and thus, simplifying control plane operations. On the contrary, a fully-reconfigurable (FR) network design, where IP routing can be also modified, would increase the burden in the control plane, but at a higher CapEx reduction, since the optical infrastructure is used more efficiently. In this work, we investigate the CapEx overprovision introduced by SR–VTCA with respect to a FR scheme. In order to do this, SR–VTCA planning problem is first modeled as a MILP formulation. A heuristic procedure based on traffic domination is then proposed to solve large instances of the problem. Exhaustive experiments are conducted comparing the SR–VTCA solutions obtained by the aforementioned MILP and heuristic proposal with solutions found by other optimization methods presented in the literature to solve the FR planning problem. Finally, the results show that SR–VTCA can achieve similar results to the FR case in terms of CapEx reduction, while a huge number of IP reroutings are saved by maintaining IP stability. Thus, SR–VTCA provides an advantageous balance between CapEx overprovisioning and the control plane overhead associated with IP rerouting.     

Novel Design of RNA Aptamers as Cancer Inhibitors and Diagnosis Targeting the Tyrosine Kinase Domain of the NT-3 Growth Factor Receptor Using a Computational Sequence-Based Approach

Aptamers, the nucleic acid analogs of antibodies, bind to their target molecules with remarkable specificity and sensitivity, making them promising diagnostic and therapeutic tools. The systematic evolution of ligands by exponential enrichment (SELEX) is time-consuming and expensive. However, regardless of those issues, it is the most used in vitro method for selecting aptamers. Therefore, recent studies have used computational approaches to reduce the time and cost associated with the synthesis and selection of aptamers. In an effort to present the potential of computational techniques in aptamer selection, a simple sequence-based method was used to design a 69-nucleotide long aptamer (mod_09) with a relatively stable structure (with a minimum free energy of −32.2 kcal/mol) and investigate its binding properties to the tyrosine kinase domain of the NT-3 growth factor receptor, for the first time, by employing computational modeling and docking tools.     

基于CMOS图像传感器的成像系统设计

以OV 5 116CMOS图像传感器为例 ,讨论了基于CMOS图像传感器的成像系统的电路设计方法及系统设计中应注意的问题 ;并通过对CMOS图像传感器外围电路的优化设计实现了成像系统的微型化和轻量化。     

基于Moodle和PhoneGap的移动学习客户端的设计与应用研究

本文将结合用户移动学习现状及偏好,确定移动学习客户端的系统需求,进而基于Moodle和PhoneGap平台,实现对移动学习客户端的设计,并将移动学习客户端应用到实际教学过程中,确定应用成效。