Resveratrol-induced cell inhibition of growth and apoptosis in MCF7 human breast cancer cells are associated with modulation of phosphorylated akt and caspase-9

Resveratrol (trans-3,4N,-5-trihydroxystilbene), a phytoalexin present in grapes and red wine, is emerging as a natural compound with potential anticancer properties. Here we show that resveratrol affects the growth of human breast cancer cell lines MCF7, MDA-MB-231, SK-BR-3, and Bcap-37 in a dose-dependent manner and that MCF7 is the most sensitive among the four cell lines. MCF7 cells treated with resveratrol showed typical characteristics of apoptosis including the poly (ADP-ribose) polymerase cleavage, TdT-mediated dUTP nick end labeling-positive staining, and morphologic changes. Phosphorylation of the oncogene product Akt was significantly reduced followed by decreased phosphorylation and increased processing of pro-caspase-9 on resveratrol treatment. These results indicate that resveratrol seems to exert its growth-inhibitory/apoptotic effect on the breast cancer cell line MCF7 via the Akt-caspase-9 pathway.     

Synthesis and antitumor activity evaluation of some novel pyrazolotriazine derivatives

6-Aminopyrazolo[1,2-a][1,2,4]triazine-4,8-dione derivative 3 was obtained upon the reaction of the acid hydrazide derivative 2a with ethyl cyanoacetate. The reactions of 3 with several electrophiles such as aldehydes, isatin, acetic anhydride, phenyl isocyanate, benzoyl isothiocyanate, and p-toluenesulfonyl chloride were studied. The structures of the newly synthesized compounds were established on the basis of IR, ¹H NMR, mass spectra, and elemental analyses. The antitumor activities of some selective compounds were examined against two cell lines as liver carcinoma cell line (HEPG-2) and human breast cancer cell line (MCF7).     

On the absolute efficiency of large neutron detectors for μCF

The absolute efficiency for a large organic scintillator neutron detector has been calculated via Monte Carlo simulation for neutrons of 14 MeV. The influence of the environmental geometry (source scattering, etc.) has been taken into account. Full differential cross-sections, including resonances, have been used. The calculated response function compares well with the measured one. This revised version was published online in August 2006 with corrections to the Cover Date.     

紫杉醇诱导的乳腺癌MCF-7细胞核凋亡的光电化学表征

利用光电分析法研究了紫杉醇诱导的非细包体系中ＭＣＦ－７细胞核的凋亡。在ＭＣＦ－７细胞核凋亡过程中,光电流的降低与ＤＮＡ断裂和细胞核形态学的变化结果相一致与传统的琼脂糖凝胶电泳法和荧光显微镜观察法相比,光电分析法能快速灵敏地在早期检测到细胞核调亡的动力学信息,并适时追踪细胞核凋亡的整个过程。     

基于MCF5213及Zigbee无线技术的音频对讲系统

本文介绍了基于Freescale Coldfire处理器系列的MCF5213芯片,以及Zigbee无线通讯技术实现无线对讲系统的参考设计.     

Azo dye with nitrogen donor sets of atoms and its metal complexes: Synthesis,characterization, DFT,biological, anticancer and Molecular docking studies

An azo derivative was synthesized by coupling diazotized 2,6‐diaminopyridine with p‐dimethyl amino benzaldehyde and this new ligand formed a series of metal complexes with Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) salts. These complexes were characterized on the basis of elemental analyses, molar conductance, infrared spectroscopy, UV–Vis, ¹H NMR, mass spectrometry, electronic spectra, magnetic susceptibility and ESR spectral studies, conductivity measurements, thermogravimetric analyses (TG‐DTG). The molecular and electronic structure of the azo ligand was optimized theoretically and the quantum chemical parameters were calculated. The ligand and its metal complexes were subjected to X‐ray powder diffraction study. The thermal stability of the ligand and its metal complexes was examined by thermogravimetry. The ligand and its complexes were tested for their in vitro antimicrobial activity, some of the complexes showed good antimicrobial activities against some selected bacterial and fungal strains. Anticancer activity of the ligand and its metal complexes are evaluated against human cancer (MCF‐7 cells viability). Molecular docking was used to predict the binding between azo ligand and the receptors of nucleoside diphosphate kinase of Staphylococcus aureus (3Q8U) and (3HB5) which is breast cancer mutant oxidoreductase. The docking study provided useful structural information for inhibition studies.     

Theoretical studies of new Schiff base ligand derived from 1,3‐diaminopropane and 2‐acetyl ferrocene and studying some applications of its metal complexes

A novel bidentate Schiff base ligand (L) and some d‐transition metal chelates (Cr (III), Mn (II), Fe (III), Co (II), Ni (II), Cu (II), Zn (II) and Cd (II)) were synthesized and characterized using various physicochemical and spectroscopic techniques like elemental analysis, IR, mass, UV–visible and thermal analysis. The spectroscopic data suggested that the parent Schiff base ligand coordinated to the metal ions through both imine nitrogen atoms. The molecular and electronic structure of the free ligand was optimized theoretically, and the quantum chemical parameters were calculated. The molecular structure can be used to investigate the coordination sites and the total charge density around each atom. The free ligand and its complexes were screened for their antimicrobial activities for various pathogenic bacteria and fungi. The anticancer activities of the free ligand, Cr (III), Mn (II) and Fe (III) complexes were screened against MCF‐7 cell line and found that Mn (II) complex has the lowest IC₅₀ (15.90 μg/ml). Molecular docking was used to predict the binding between the free ligand with receptor of mutant human androgen (ARccr) derived from androgen‐independent prostate cancer (1GS4), crystal structure of yeast‐specific serine/threonine protein phosphatase (ppz1) of Candida Albicans (5JPE) and crystal structure of renal tumor suppressor protein, folliculin (3 V42) and to identify the binding mode and the crucial functional groups interacting with the three proteins.     

Non–symmetrically p–nitrobenzyl–substituted N–heterocyclic carbene–silver(I) complexes as metallopharmaceutical agents

In the present work, a series of eight new imidazole, 4,5–dichloroimidazole, 4,5–diphenylimidazole and benzimidazole based nitro–functionalized mono–N –heterocyclic carbene (NHC)–silver(I) acetate ( 7a–d ) and bis–NHC–silver(I) hexafluorophosphate complexes ( 8a–d ) were synthesised by the reaction of the corresponding azolium hexafluorophosphate salts ( 6a–d ) with silver(I) acetate and silver(I) oxide in methanol and acetonitrile, respectively. All the synthesised compounds were fully characterized by various spectroscopic techniques and elemental analyses. Additionally, the structure of bis–(1–benzyl–3–(p –nitrobenzyl)–4,5–dichloroimidazole–2–ylidene)silver(I) hexafluorophosphate complex ( 8b ) was confirmed by single crystal X–ray diffraction analysis. Preliminary in vitro antibacterial evaluation was conducted for all the compounds ( 6a–d) , ( 7a–d) , and ( 8a–d) by Kirby–Bauer's disc diffusion method followed by the determination of Minimum Inhibitory Concentration (MIC) from broth macrodilution method against five standard bacteria; two Gram–positive bacterial strains (Staphylococcus aureus and Bacillus subtilis) and three Gram–negative bacterial strains ( Escherichia coli , Shigella sonnei, and Salmonella typhi). All the hexafluorophosphate salts ( 6a – d) were found inactive against the tested bacterial strains and their corresponding mono– and bis–NHC–silver(I) complexes ( 7a–d and 8a–d ) exhibited moderate to high antibacterial activity with MIC value in the range 8–128 μg/mL. In addition, preliminary in vitro anticancer potential of all the silver(I) complexes ( 7a–d and 8a–d ) was determined against the human derived breast adenocarcinoma cells (MCF 7) by MTT assay. All the mono– and bis–NHC–silver(I) complexes ( 7a–d and 8a–d ) orchestrated high anticancer potential with IC₅₀ values ranging from 10.39 to 59.56 nM. In comparison, mono– NHC–silver(I) complexes performed better than the bis–NHC–silver(I) complexes.     

基于多芯光纤错位熔接结构的温度与折射率同时测量传感器

提出了一款由输入单模光纤(SMF)-多芯光纤(MCF) -多模光纤(MMF)-输出SMF错位熔接构成的光纤Mach-Zehnder 干涉仪(MZI)结构,实现了环境温度和折射率的同时测量。实验结果表明,随着环境温度的 变化,传感器透射谱峰值波长线性漂移,灵敏度达 到54.238pm/℃,而透射谱峰值强度随温度变化不敏感;随着环境折 射率的变化,传感器透射谱峰值 强度线性变化,灵敏度达到10.704dB/RIU(RIU为折射率单位),而透 射谱峰值波长随环境折射率变 化不敏感。通过同时监测传感器输出光谱的峰值波长漂移量和峰值强度变化量,能够实现温 度与折 射率的同时测量。在温度与折射率测量时分别利用波长调制法与强度调制法,因此它们同时 测量不 存在交叉感染。本文传感器具有结构简单、制作容易和灵敏度高等优点,在生物、化学和医 学等领域有广泛的应用前景。     

嵌入式微处理器MCF5249及其应用

文中介绍了MOTOROLA公司的嵌入式微处理器MCF5249的原理、特点和引脚功能，说明了基于该处理器和嵌入式操作系统UCLINUX的网络相机结构，给出了用MCF5249进行网络相机设计时的资源分配情况及电路设计注意事项。