便于硬件实现的去隔行算法研究

在比较几种去隔行算法的基础上,提出了两种便于硬件实现的去隔行算法:边增强窗函数算法和综合中值滤波算法.前一种算法采用双滤波器结构,既保证了图像的清晰度又使其边界锐利,获得了较好的视觉效果.后一种算法综合了几种中值滤波算法的优势,克服了原来算法去隔行时的缺点.软件仿真表明,图像的测评效果良好.两种算法的硬件实现方便,成本较低,具有实用价值.     

户式中央空调控制系统温度采集模块的设计应用

提出了一种应用于户式中央空调温度采集模块的软硬件设计方法.说明了通过软件方法实现热敏电阻的阻值随温度非线性变化这种特性的线性化.经过实际测量,验证了该模块设计的可行性和有效性.     

基于无线传感器网络的油井远程监控系统设计

现有的油井抽油机远程监控系统建设费用昂贵且存在通信盲区的问题,针对这一状况设计了一种基于无线传感器网络的油井监控系统.采用无线微处理器JN51 39构建基于ZigBee协议的无线监测网络;结合GPRS模块MC55构建网关节点实现数据汇聚和GPRS通信方式的远程数据转发.该系统工作性能稳定,将其应用在油田可实现油田生产管理的信息化和自动化.     

基于MC145170和BA1404芯片的锁相环调频发射机的研制

介绍了一种基于频率合成器芯片MC145170和调频发射芯片BA1404构成的调频发射机的设计方法。该方法由于采用了锁相环路PLL,因此,其设计的调频发射机具有频率稳定度高、调整便捷等优点。     

MC10116放大器在波形转换中的应用

振荡频率测量电路中的振荡信号常常是含有不同频率的正弦波．因而不便于数字频率的测试且变化较大。文中介绍了一种利用可测试长距离不同信号的三路放大器MC10116来将正弦波换转成方波,从而通过产生适于测量的高低电平来测量频率的具体电路和实现方法。     

Direct determination of δ44/42Ca isotope ratio by ion chromatography/low‐resolution multicollector ICPMS

High‐precision on‐line procedure for measurement of calcium isotopic ratio by coupling ion chromatography to multicollector inductively coupled plasma mass spectrometry was developed. Calcium separation from the sample matrix was achieved on an ion chromatography column—IonPac CS16—ID 3 mm connected with CERS 500 2 mm suppressor and followed by multicollector inductively coupled plasma mass spectrometry calcium isotopic ratio determination. Dry plasma mode was used with Aridus II desolvation system. To sustained samples with high level of total dissolved salts as well as account capacity of applied analytical column, the method has been optimized regarding calcium isotope ratio measurements with low‐resolution mass spectrometry. Mass discrimination and instrument drift were corrected by sample‐standard bracketing method using the ⁴⁴Ca/⁴²Ca isotope ratio of SRM 915a as a standard. Good accuracy and reasonable precision of calcium isotope ratio (generally 0.20‰ [2SD]) were achieved, which are comparable to off‐line Ca separation and continuous measurement. The reproducibility of the proposed analytical procedure was verified by measuring the SRM 915a standard as a sample randomly over 3 months (n = 56). Applicability of the protocol was demonstrated for matrix‐rich natural water samples, coral samples, and bone standard reference materials.     

钙伯三醇的关键中间体——麦角甾烷-5，7（E），10（19）-三烯-6（S）-二氧化硫的合成

麦角甾烷-5,7(E),10(19),22(E)-四烯-6(S)-二氧化硫(1)经臭氧化反应合成了钙伯三醇(MC903)的重要中间体--麦角甾烷-5,7(E),10(19)-三烯-6(S)-二氧化硫(2),其结构经1H NMR和13C NMR表征.较佳的臭氧化反应条件为:1 1.0 mmol,溶剂为V(二氯甲烷):V(甲醇)=4:1,于-65℃～-78℃反应12 min.在此条件下,2中醛基α-位的差相异构化降至最低程度,产率90%.对差相异构化的机理进行了讨论.     

铸型尼龙/纳米ZnO复合材料的制备与表征

采用原位聚合反应制备MC尼龙6/纳米ZnO复合材料并对其性能、形貌和结晶形态进行了分析.分析结果表明:MC尼龙6/纳米ZnO复合材料中ZnO达到了纳米级分散,并且粒子分布比较均匀,粒子的粒径在30~50 nm左右.纳米ZnO没有改变尼龙6的结晶形态.纳米ZnO的加入起到同时增强增韧的作用;使体积电阻系数降了8×104倍、表面电阻系数降了5.7×104倍、摩擦系数降低了29%,MC尼龙6/纳米ZnO复合材料摩擦磨损性能和抗静电性能明显优于MC尼龙6;经过力学拉伸试验的破坏,引起MC尼龙6结构发生宏观应力变化,造成(002 202晶面)产生明显的择优取向.     

A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody

Microcystins (MC) are cyanobacterial hepatotoxins responsible for animal-poisoning and human health incidents. Immunoassays provide a sensitive and fast means to detect these toxins, but cross-reactivity (CR) characteristic of different antibodies was variable. Here, we have produced and characterized a monoclonal antibody (Clone MC8C10) with highly specificity against the most frequent and most toxic variant of microcystins, MC-LR. MC8C10 is more specific against MC-LR among the reported antibodies before. The immunogen was synthesized from the modified MC-LR and bovine serum albumin (BSA). An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with MC8C10 was established to detect the MCs in waters, which showed highly specificity with MC-LR, and have a detection limit for MC-LR 0.1 μg L⁻¹, the 50% inhibition concentration (IC₅₀) for MC-LR was 1.8 ± 0.1 μg L⁻¹ and the quantitative detection range was from 0.3 to 10 μg L⁻¹. The [4-arginine] microcystins and the nodularin-R showed lower cross-reactivates (CR < 10%), and other MCs such as MC-LF and MC-LW are not recognized (CR < 10⁻⁴). The analysis results of real water samples with ic-ELISA showed that all the coefficients of variation were less than 15%, and the recovery was (100.3 ± 5.9)%. So the highly specific ic-ELISA will commendably suit for sensitive analysis for MC-LR in surface water as well as drinking water.     

K-Carrageenan Stimulates Pre-Osteoblast Proliferation and Osteogenic Differentiation: A Potential Factor for the Promotion of Bone Regeneration?

Current cell-based bone tissue regeneration strategies cannot cover large bone defects. K-carrageenan is a highly hydrophilic and biocompatible seaweed-derived sulfated polysaccharide, that has been proposed as a promising candidate for tissue engineering applications. Whether κ-carrageenan can be used to enhance bone regeneration is still unclear. In this study, we aimed to investigate whether κ-carrageenan has osteogenic potential by testing its effect on pre-osteoblast proliferation and osteogenic differentiation in vitro. Treatment with κ-carrageenan (0.5 and 2 mg/mL) increased both MC3T3-E1 pre-osteoblast adhesion and spreading at 1 h. K-carrageenan (0.125–2 mg/mL) dose-dependently increased pre-osteoblast proliferation and metabolic activity, with a maximum effect at 2 mg/mL at day three. K-carrageenan (0.5 and 2 mg/mL) increased osteogenic differentiation, as shown by enhanced alkaline phosphatase activity (1.8-fold increase at 2 mg/mL) at day four, and matrix mineralization (6.2-fold increase at 2 mg/mL) at day 21. K-carrageenan enhanced osteogenic gene expression (Opn, Dmp1, and Mepe) at day 14 and 21. In conclusion, κ-carrageenan promoted MC3T3-E1 pre-osteoblast adhesion and spreading, metabolic activity, proliferation, and osteogenic differentiation, suggesting that κ-carrageenan is a potential osteogenic inductive factor for clinical application to enhance bone regeneration.