Highly sensitive determination of haloperidol in human serum by capillary gas chromatography using a surface ionization detector

A method has been developed for highly sensitive determination of haloperidol in human serum involving a simple extraction procedure followed by gas chromatographic separation. Target components were separated from the extracting solvents with a Van den Berg type solventless sample injector before introduction Into a DB-1 capillary separation column. A surface ionization detector (SID), which has highly selective sensitivity for Substituted amines, was employed for quantitation using bromperidol as an internal standard. Chloroform proved to be the best extracting solvent, yielding a quantitative detection limit of 5 ng/ml (S/N = 2). Comparison of the response to target compounds obtained by the SID, FTD (flame thermionic detector), and FID (flame ionization detector) showed the SID to be superior.     

原子结构参数的改进Xa法计算

六十年代后期,在Hartree－Fock-Slater法的基础上,提出了Xα法［1］．用于原子结构计算的Xα法与HF（Hartree－Fock）法的主要区别在于：用简单的统计平均交换势替代了HF法中计算最为困难的电子交换势,从而在保持较高理论严谨性和计算精确度的同时,大大减少了计算工作量,近年来获得了广泛的应用．我们尝试用经过适当修改的Xα方法,计算原子参数,解决分子结构中的某些问题．用原子参数解决分子问题,历来是化学和物理工作者常用的方法．本工作的意图是引入一个比HF法简单的容易在微机上实现的某种表现原子参数的计算方法,提供…     

Elucidating dominant factors of PO43-, Cd2+ and nitrobenzene removal by biochar: A comparative investigation based on distinguishable biochars

Four distinct biochars were employed to remove three typical pollutants, meanwhile, path analysis, a multi-statistical regression method, was performed to elucidate the dominant factors of biochar adsorption. This work can provide a new insight to prepare a targeted biochar as adsorbents.     

Fraktionierte Kristallisation von Ammonium- und Magnesiumdoppelnitraten der Ceriterden; Bestimmung der Trennfaktoren und Vergleich der Trennwirkung

Summary The Separation Factors for Pr/La, Nd/Pr, and Sm/Nd, characterizing the crystallization of ammonium and magnesium double nitrates under practical conditions, were determined analytically and are discussed. They confirm that La and Pr can be separated better by fractional crystallization of ammonium double nitrates, whereas in the presence of Sm the magnesium double nitrates are preferable.

     

固定HCG抗体膜的跨膜电位

高分子膜作为一项新兴技术,在很多领域得到日益广泛的应用.近十几年,随着生物工程和生物传感器的迅速发展,高分子生物功能膜的研究倍受重视.高分子生物功能膜是采用固定化技术,将具有分子识别功能的材料(如酶、抗原、抗体等)固定在高分子膜上而制得的.在固定化膜表面发生的生物化学反应,可以引起膜的荷电状态的变化,从而导致跨膜电位的变化。有关固定化膜的报导较多,但主要限于固定化的方法及其应用方面的研究,而有关高分     

Studies on Interactions of Antibiotics with Serum Albumin by Surface Plasmon Resonance Biosensor

Introduction Humanserumalbumin(HSA)isawell known transportproteinforavarietyofmoleculesandions[1].Thebindingofadrugtoserumalbuminhasimportant pharmacokineticconsequencesbecauseitinfluences distribution,excretionandpharmacologicaleffectsof thedruginthebody…     

增强化学发光体系酶联免疫分析法测定人绒毛膜促性腺激素

应用活化鲁米诺,用优化的增强化学发光酶联免疫分析体系测定人绒毛膜促性腺激素,检测限为0.2mIU/ml。线性范围0～200mIU/ml,与放射免分析测定结果比较,相关性良好。进而又发展了一种半定量的照相测定法,通过实际血清样品测定,效果良好。     

Cathodic stripping voltammetric detection and determination at a hanging mercury-drop electrode of dye contaminants in purified biomaterials: study of the human serum albumin and reactive dye 120 system

Procion red HE-3B (RR120) is an example of dye currently used in affinity purification. A method is described for determining trace amounts of RR120 dye contaminant in human serum albumin by cathodic stripping voltammetry. The method is based on a measure of a well-defined peak at −0.58 V, obtained when samples of HSA protein (0.01-2% w/v) containing dye concentrations are submitted to a heating time of 330 min at 80 °C in NaOH, pH 12.0 and the samples are removed to a solution containing Britton-Robinson buffer, pH 4.0. Using an optimum accumulation potential and time of 0 V and 240 s, respectively, linear calibration curves were obtained from 1.0×10⁻⁹ to 1.0×10⁻⁸ mol l⁻¹ for RR120 dye. Leakage/hydrolysis of reactive red 120 from an agarose support (e.g. at pH 2 or 12) can also be conveniently determined at very low levels (sub-μg ml⁻¹) by means of cathodic stripping voltammetry, which involves adsorptive accumulation of the dye onto the hanging mercury-drop electrode.     

Direct and simultaneous spectrofluorometric determination of naproxen and salicylate in human serum assisted by chemometric analysis

A direct method for the simultaneous determination of naproxen and salicylate in human serum is reported, based on a combination of spectrofluorometric measurements with two multivariate calibration techniques: partial least-squares (PLS-1) and the novel net analyte preprocessing (NAP). The method is rapid, selective and sensitive, and is based on the measurement of the fluorescence spectra of NH₃ alkalinized whole human sera at the excitation wavelength of 315 nm. It can be applied within the ranges of concentrations 50-200 ng ml⁻¹ for naproxen and 100-300 ng ml⁻¹ for salicylate. The employed chemometric techniques have been compared on the basis of the statistical indicators for calibration and validation. Reproducibility and interference studies in abnormal sera have also been carried out.     

Voltammetric determination of 5-hydroxydole-3-acetic acid in human gastric juice

A method for the determination of the major serotonin metabolite — 5-hydroxyindole-3-acetic acid (5-HIAA) in human gastric juice by cyclic voltammetry was described. The measurement conditions were investigated. The potential window was chosen from +0.1 to +0.9 V, the supporting electrolyte was 0.025 M PBS solution (pH 2.0). The method allowed determination in the concentration range from 2.0 ×10⁻⁷ to 2.0 ×10 ⁻⁵ M and a detection limit of 80 nM. When samples of gastric juice were analyzed with the method, we obtained the mean content of 5-HIAA in the gastric juice. Meanwhile, interference from other ions and substances were examined. The experimental results indicate that the method for the determination of gastric juice samples is successful.