Effect of purified fractions from cell culture supernate of high‐density pre‐B acute lymphoblastic leukemia cells (ALL3) on the growth of ALL3 cells at low density

The mechanisms underlying the aberrant growth and interactions between cells are not understood very well. The pre‐B acute lymphoblastic leukemia cells directly obtained from an adult patient grow very poorly or do not grow at all at low density (LD), but grow better at high starting cell density (HD). We found that the LD ALL3 cells can be stimulated to grow in the presence of diffusible, soluble factors secreted by ALL3 cells themselves growing at high starting cell density. We then developed a biochemical purification procedure that allowed us to purify the factor(s) with stimulatory activity and analyzed them by nanoliquid chromatography‐tandem mass spectrometry (nanoLC‐MS/MS). Using nanoLC‐MS/MS we have identified several proteins which were further processed using various bioinformatics tools. This resulted in eight protein candidates which might be responsible for the growth activity on non‐growing LD ALL3 cells and their involvement in the stimulatory activity are discussed.     

Electrochemical Characterizations of four Main Redox–metabolites of Pseudomonas Aeruginosa

Bacterial identification is of first importance in clinic nowadays. For few years, electrochemistry appears as a reliable route for characterizations outside of laboratories. Nowadays, researchers mainly focus on the opportunistic pathogen Pseudomonas aeruginosa because of its production of the Pyocyanin toxin which has an electrochemical case study behavior. Other P. aeruginosa secreted molecules are also studied in a lesser extent. This work deals with the systematic electrochemical characterizations in aprotic and protic solvents of 4 main metabolites of this bacterium in the view of multispecies detection of P. aeruginosa . We report here the behavior of the 2‐Heptyl‐4(1H)‐quinolone (HHQ), Pseudomonas Quinolone Signal (PQS), Pyocyanin (PYO) and the 2′aminoacetophenone (2‐AA). All the mentioned species are clearly visible by using electrochemical techniques (cyclic and square wave voltammetries). The 2 most suitable species for electrochemical detection appear to be PQS and PYO because of their detection at low potential.     

On the Relative Gain Array (RGA) with singular and rectangular matrices

This paper identifies a significant deficiency in the literature on the application of the Relative Gain Array (RGA) formalism in the case of singular matrices. Specifically, it is shown that the conventional use of the Moore–Penrose pseudoinverse is inappropriate because it fails to preserve critical properties that can be assumed in the nonsingular case. It is then shown that such properties can be rigorously preserved using an alternative generalized matrix inverse.     

Sustainable alternative in environmental monitoring using carbon nanoparticles as optical probes

Environmental monitoring is getting more important nowadays due to the greater stress faced by the natural environment in the era of urbanisation and industrialisation. To accomplish the task, rapid and reliable analytical probes are essentially needed to perform the monitoring at real time basis with high sensitivity and accuracy. In view of this, analytical probes developed using carbon nanoparticles are one of the latest alternatives that are proven with capability to detect various analytes of the environment. Carbon nanoparticles portray good fluorescence property that enables the integration onto optical sensing transducers. Further engineering via surface functionalization can be performed in the interest to improve the selectivity and sensitivity of the probes. There are several advantages of using carbon nanoparticles and the most significant benefit is the sustainability prospect as compared to other groups of fluorophores. Carbon nanoparticles can be synthesised with greener approach via simple pyrolysis or hydrolysis processes that involve minimum use of toxic or harmful starting precursors, besides able to tap on using renewable resources such as carbon rich agricultural wastes. The synthesis is often performed under mild condition and produces less or no side chemical products. Carbon nanoparticles by nature show low toxicity effect to the environment. This review focuses specifically of the sustainable significances, advantages and achievements in adopting carbon nanoparticles as an alternative for environmental monitoring.     

Imidazolium Based Surface Active Ionic Liquids as Novel Micellar Media for Simultaneous and Sensitive Electrochemical Detection of Dopamine and Ascorbic Acid

Surface active ionic liquid (SAIL) micelle assisted, simultaneous and highly sensitive electrochemical sensing of dopamine (DA) and ascorbic acid (AA) is presented. Results presented herein establish that SAILs viz.1‐dodecyl‐3‐methyl imidazolium chloride ([DDMIM][Cl]), 1‐octyl‐3‐methyl imidazolium chloride ([OMIM][Cl]) and 1‐butyl‐3‐methyl imidazolium chloride ([BMIM][Cl]) exhibit a probe and SAIL nature/concentration specific impact on the redox behaviour of hydroquinone (H₂Q), dopamine (DA) and ascorbic acid (AA). To our observations, the electrochemical behaviour of DA and AA is affected oppositely by SAILs with the apparent effects being more appreciable in presence of [DDMIM][Cl]. In the presence of [DDMIM][Cl] micelles, the electro‐oxidation of AA was observed to occur at potentials about 350 mV less positive than required for electrooxidation of DA, an important advantage that minimises the interference of former in sensing of the later. The peak to peak potential separation of 350 mV observed in presence of [DDMIM][Cl] micelles is the largest to be reported so far. The DPV signal for DA and AA displayed a linear response in the concentration range of 6.6 to 99.9 μM and 6.6 to 131.5 μM respectively. Very low detection limits of 0.0161 μM for DA in presence of 39.8 μM AA and 0.0227 μM for AA in presence of 39.8 μM DA were estimated in micellar phase of [DDMIM][Cl].     

基于现场可编程门阵列单探测器复合轴控制技术

为了解决空间激光通信系统中单探测器复合轴结构的粗精解耦难题,通过分析二维关联控制矩阵,得出粗精跟踪解耦的必要条件,提出了一种单探测器复合轴结构的工作方式.在跟踪过程中,相机直接配合子轴执行器完成精跟踪工作,而主轴的跟踪信息由子轴的角度偏转器来提供,同时所有的图像处理算法、位置检测算法、以及粗精伺服控制算法都在现场可编程门阵列中实现,完成了系统的轻小型化设计.实验搭建了测试系统,并对现场可编程门阵列硬件和系统跟踪性能进行测试,结果表明单探测器复合控制系统可以实现跟踪准确度优于3μrad,为工程化奠定了良好的基础.     

基于行列式随机循环的压缩感知测量矩阵研究

压缩感知理论,从信号的自身特性出发,通过变换作用域和线性投影实现对信号的采样和压缩。测量矩阵是该理论中获得最优测量,实现精确重构的关键。本文在介绍常用测量矩阵的基础上,重点研究了结构化测量矩阵。鉴于测量矩阵设计的最重要的原则是降低矩阵元素间的相干性,本文借鉴循环矩阵和广义轮换矩阵的优点,提出了采用均匀随机数对结构化测量矩阵进行随机循环的构造方法。仿真实验表明新矩阵在信号重建上具有更好的性能。     

裸眼3D系统SD卡FPGA读取显示研究

SD卡可为便携式裸眼3D设备提供数据来源,图像存储于SD卡中,FPGA以SPI协议方式读出,再经过FIFO和SDRAM进行数据缓存,通过接口输入显示器显示.探讨了通过FPGA器件读取SD卡图像进行显示的实现方法,得出了SD卡文件读取及显示的可行逻辑设计.系统性能经过初步验证,可行性良好.     

The Formation of Luminescent Supramolecular Ternary Complexes in Water: Delayed Luminescence Sensing of Aromatic Carboxylates Using Coordinated Unsaturated Cationic Heptadentate Lanthanide Ion Complexes

The synthesis of four lanthanide ion complexes Eu?1, Eu?2, Tb?1 and Tb?2, from the heptadentate tri-arm cyclen (1,4,7,10-tetraazacyclododecane) ligands 1 and 2 that were made in one-pot syntheses is described. These coordinatively unsaturated complexes have two labile metal-bound water molecules, as demonstrated by X-ray crystallography. This was also confirmed by evaluating their hydration state (q～2) by measuring their lifetimes in D²O and H²O, respectively. The above complexes were all designed as being “photophysically silent” prior to the recognition of the anion, since they do not possess antenna that can participate in sensitisation of the Eu(III) or the Tb(III) excited state. However, the two water molecules can be displaced upon anion binding by the appropriate aromatic carboxylates to give ternary complexes in water, through either four- or six-member ring chelates (q～0), or possibly via a monodentate binding. In the case of Tb?1 and Tb?2, large luminescence enhancements were observed upon the formation of such ternary complexes with N,N-dimethylaminobenzoic acid at ambient pH. Such binding and luminescent enhancements were also observed for Tb?1 in the presence of salicylic acid. On all occasions, the anion recognition “switched” the emission “on” over two logarithmic units. At higher concentrations, the emission is reduced possibly due to quenching. In the case of aspirin, the binding was too weak to be measured, indicating that Tb?1 selectively detects salicylic acid, the active form of aspirin in water. In the case of Eu?1 and Eu?2, the affinity of these complexes towards such aromatic carboxylates was too weak for efficient ternary complex formation.