Proteome analysis of altered proteins in streptozotocin‐induced diabetic rat kidney using the fluorogenic derivatization–liquid chromatography–tandem mass spectrometry method

To find new molecular markers for early diagnosis of diabetic nephropathy, we applied fluorogenic derivatization–liquid chromatography–tandem mass spectrometry to identify the differentially expressed proteins in the kidney of control and streptozotocin‐induced diabetic rats. The Sprague–Dawley rats were injected with the sodium citrate buffer or streptozotocin and then killed after 1, 4, 12 and 24 weeks. The results showed that seven proteins were significantly changed after 1 week of injection. Only one protein had significantly changed after 4 weeks of injection. However, after 12 weeks of injection, the number of altered proteins rose to 10. After 24 weeks of injection, 18 proteins had altered significantly. Five common proteins were significantly altered at week 12 and 24 after injection, respectively. Importantly, these proteins appeared prior to microalbuminuria and may serve as new biomarkers that are able to improve early detection of and new drug development for diabetic‐related nephropathy. Copyright © 2012 John Wiley & Sons, Ltd.     

基于DSP的稳定平台设计

为了消除海水运动对需要保持稳定姿态物体的影响,设计了两轴稳定平台。该稳定平台以TMS320F28335DSP为核心微处理器,采用了多传感器采集、伺服控制技术等;并架设了嵌入式操作系统μC／OS-Ⅱ来管理多任务稳定平台系统。实验结果表明,该两轴稳定平台在稳定性及动态性能上均能满足要求,起到了隔离海水运动的目的。     

Simple Multiresidue Determination of Fluoroquinolones in Bovine Milk by Liquid Chromatography with Fluorescence Detection

Abstract

A simple and sensitive method for the simultaneous determination of five fluoroquinolones (ciprofloxacin, enrofloxacin, danofloxacin, difloxacin, and sarafloxacin) in bovine milk was developed. Protein precipitation from milk samples was achieved by the addition of acetonitrile and o‐phosphoric acid. Acetonitrile was removed with dichloromethane, leaving the fluoroquinolones in the acid aqueous extract. The aqueous extract was analyzed by liquid chromatography with fluorescence detection (LC–FD). The mobile phase was composed of acetonitrile and 10 mM citrate buffer solution of pH 4.5, with an initial composition of acetonitrile‐water (12∶88, v/v) and using linear gradient elution. Norfloxacin was used as an internal standard. The limits of detection found ranged from 1 to 6 ng · mL^?1 and were below the maximum residue limits (MRLs) established by the European Union. The proposed method was applied to the determination of these compounds in different bovine milk samples. Method validation was carried out by a recovery assay.     

基于DSP的地下微水检测系统

根据微水试验法确定含水层水文地质参数的实验要求,介绍一种基于DSP的地下微水检测系统,用于观测井中水位降深随时间的变化规律。该系统以TMS320F2812为核心,采用压力式液位变送器和温度传感器进行数据采集,数据经分析处理后在LCD上实时显示,同时通过USB接口存储数据。该系统采用 μC／OS-Ⅱ作为底层软件平台,实现功能模块程序设计。现场运行结果表明：系统结构简单,实时性好,可靠性高,测量精度可达到2‰。     

基于ARM处理器和μC/OS-Ⅱ操作系统的电子机箱智能监控技术

研究了基于ARM处理器和μC/OS-Ⅱ操作系统对VPX电子机箱实时智能监控的技术。完成了故障监测收集、控制、存储和上报,解决了VPX电子机箱故检和监控的技术问题,实现了VPX电子机箱的智能监控,方便故障定位和维修。     

一种基于积分求导(DQ)的时域有限差分法(TD-FD)

本文提出用DQ(Differential Quadrature)将Maxwell方程中的部分偏微分进行近似计算,从而减少时域有限差分法(TD-FD)算法中的网络节点数目,节省计算机的内存,提高TD-FD的分析效率.     

多载波频率分集扩展频谱通信的同步原理分析

多载波频率分集扩展频谱通信系统（ＦＤ／ＳＳＭＡ）是基于多载波传输技术上的一种新的扩展频谱多址系统。本文分析研究该系统的同步原理，其中包括接收机参考信号与所接收信号的定时同步的识别、定时同步的跟踪、载波频率跟踪及载波相位跟踪。分析表明，通过三个误差信号，即定时同步误差信号、载波频率误差信号及载波相位误差信号的获取及它们对系统的控制，从原理上解决了ＦＤ／ＳＳＭＡ系统的同步问题     

Spread SCPC/FDMA,spread TDMA/FDMA and SSMA/FDMA transmission through A non-linear satellite channel with an earth-station off-axis emission constraint

This paper analyses and compares possible access schemes to be used in satellite networks with a large number of small earth-stations. Basically, frequency division (FDMA), time division (TDMA), and spread spectrum multiple access (SSMA) are considered. For TDMA, one single carrier originated in one particular earth-station is transmitted in each sub-band and therefore a single-channel-per-carrier system results (SCPC/FDMA). For TDMA and SSMA, it is assumed that groups of earth-stations using either of these access schemes share the full available band in FDMA. The corresponding systems are thus TDMA/FDMA and SSMA/FDMA. Moreover, for SCPC/FDMA and TDMA/FDMA individual carriers may be spread to the extent that the available bandwidth is always fully occupied. Spectral efficiencies for these three access modes (Spread SCPC/FDMA, Spread TDMA/FDMA and SSMA/FDMA) are computed and compared for transmission through a non-linear satellite channel and optimum operating points for the non-linear amplifier are also determined. Comparisons are also performed for the situation in which transmitting earth-stations are bounded to obey an off-axis emission constraint.     

室内无线差分网格编码直扩多址系统的性能分析

本语言分析了室内无线多径衰落信道中差分网格编码调制直扩多址通信系统的性能，分析中采用特征函数法和留数定理计算序列差错概率，用高斯近似分析多址干扰和多径干扰，本文最后给出了误码率的理论分析结果和计算机模拟结果，并与常规的未编码直扩系统性能对比。     

μC／OS-Ⅲ在80386保护模式下的移植

文章介绍了一种将μC／OS-Ⅲ移植到Intel80386处理器上,使其能够借助T-DOS的资源,在保护模式下正常运行的方法。通过解析内核相关代码、CPU相关代码和板级支持包代码这三部分代码,讲述了将实时操作系统μC／OS-Ⅲ移植到80386处理器保护模式下的全过程。最后给出了在保护模式下运行μC／OS-Ⅲ的实例。