氦氖激光临床治疗322例观察

我室从1979年8月至1980年2月共治疗各类疾病322人，其中男172人，女150人。年龄最大76岁，最小半岁。疗程最长20次，最短3次。     

鼻内镜下YAG激光治疗咽囊囊肿

目的:目探讨在鼻内镜下YAC激光治疗咽囊囊肿的方法及疗效。方法:在鼻内镜下利用YAG激光治疗咽囊囊肿13例,1例伴有鼻息肉,鼻内镜下利用电动切削器切除,余12例在鼻内镜下利用YAG激光治疗,其中咽囊脓肿3例。结果:经1至2年随访,总有效率达100%,无并发症。结论:在鼻内镜下利用YAG激光治疗咽囊囊肿,该方法视野清晰,操作简单,出血少,病人痛苦小,门诊即可完成,疗效可靠,值得推广。     

Natural Compound Curcumin—— a Channel Potentiator Rather Than a Corrector of the Defective Intracellular Processing of △F508 Mutant Cystic Fibrosis Transmembrane Conductance Regulator

Cystic fibrosis（CF） is a severe genetic disease caused by the gene mutation of the cystic fibrosis transmembrane conductance regulator（CFTR） chloride channel. The most common point mutation AF508, which leads to impaired intracellular processing and channel gating of CFTR, appears in about 90% CF patients. The natural compound curcumin was reported to correct the processing defect of AF508-CFTR and proposed as a potential therapeutic drug to cure CF. In the present study, we analyzed the effect of curcumin on AF508-CFTR and demonstrated that curcumin can restore the impaired chloride conductance of AF508 mutant CFTR. The activity is rapid, reversible and cAMP-dependent. However, we couldn＇t reproduce the previously reported correction of the defective membrane trafficking of AF508-CFTR by curcumin. Therefore, curcumin may not be a superior lead compound for developing anti-CF drugs.     

输尿管开口囊肿、狭窄及膀胱颈部挛缩的HO:YAG激光治疗

目的利用HOYAG激光的软组织切割及止血作用,对输尿管开口囊肿、狭窄及膀胱颈部挛缩的患者行激光治疗,并观察疗效.方法采用HOYAG激光对6例输尿管开口囊肿,15例输尿管开口狭窄(其中3例合并输尿管结石),32例膀胱颈部挛缩进行治疗,观察术中出血、术后并发症、膀胱挛缩患者手术前后Qmax.结果总计53例患者通过HOYAG激光治疗,均获得满意临床疗效,术中几乎无出血,输尿管开口囊肿及狭窄患者,术毕能顺利留置F7双猪尾巴导管.膀胱颈部挛缩患者术后最大尿流率提高了7.6ml/s(7.2～14.8ml/s).结论HO;YAG激光对输尿管开口囊肿、狭窄及膀胱颈部挛缩是一种行之有效的治疗方法,且无出血,易于操作、安全、恢复快.尤其是输尿管结石合并输尿管开口狭窄患者,可通过HOYAG激光一次手术解决.     

Natural Compound Curcumin Corrects the Gating Defect of G551DMutant Cystic Fibrosis Transmembrane Conductance Regulator

In the present study, we identified the natural compound curcumin to be an effective G551D-CFTR activator by cell-based fluorescent assay and electrophysiological measurement. We demonstrated that curcumin can restore the impaired chloride conductance of G551D mutant CFTR. The activity is rapid, reversible, and cAMP-dependent. Our study identified a new natural lead compound for the pharmacological therapy of cystic fibrosis caused by G551D mutation of CFTR.     

椎管内巨大脊膜囊肿的诊断和治疗

目的探讨椎管内巨大脊膜囊肿的临床特征及手术疗效。方法本组收治17例椎管内巨大脊膜囊肿,男性9例,女性8例,年龄13～61岁,平均37.6岁。病程3个月～9年,平均1.7年。临床表现为胸背部、腰背部疼痛不适及下肢麻木疼痛者11例,骶尾会阴部麻木不适4例,大小便费力困难者2例。结果手术后临床症状均获得不同程度的改善,其中2例会阴部麻木不适和1例小便费力困难者改善不明显。术后随访3～38个月,平均13.4个月,未见囊肿复发。结论MRI检查对本病的诊断非常有意义。手术切除囊肿壁和封闭漏口效果确切。     

CO2激光加液化酚治疗皮脂腺囊肿

皮脂腺囊肿又称粉瘤,是由于皮脂腺毛囊口角化过度的阻塞,使皮脂腺的排泄物储留于腺的导管内而成。多见于青壮年男性,好发于头、面、胸、背等处,大小不等,单发     

CO2激光治疗尿道肉阜八例、尿道口囊肿一例

我院从1980年至1982年用CO2激光（输出功率10W，光斑直径0．4mm，功率密度9000w／cm^2）治疗尿道肉阜八例（女性，病史最长8年，最短2月）、尿道口囊肿一例（男性）。患者年龄29—74岁，其中7例是30—60岁。     

Synthesis and Characterization of A Small Molecule CFTR Chloride Channel Inhibitor

A thiazolidinone CFTR inhibitor (CFTRinh-72) was synthesized by a three-step procedure with tri-fluromethylaniline as the starting material. The synthesized CFTR inhibitor was characterized structurally bymeans of ^1H NMR and functionally in a CFTR-expressing cell line FRT/hCFTR/EYFP-H148Q by both fluo-rescent and electrophysiological methods. A large amount(100g) of high-quality small molecule thiazolidi-none CFTR chloride channel inhibitor, CFTRinh-72, can be produced with this simple three-step synthetic pro-cedure. The structure of the final product 2-thioxo-3-(3-trifluromethylphenyl )-5-[4-carboxyphenyl-methylene]-4-thiazolidinone was confirmed by ^1H NMR. The overall yield was 58% with a purity over 99%as analyzed by HPLC. The synthesized CFTRinh-72 specifically inhibited CFTR chloride channel function in acell-based fluorescence assay(Kd≈1.5μmol/L) and in a Ussing chamber-based short-circuit current assay(Kd≈0. 2μmol/L), indicating better quality than that of the commercial combinatorial compound. The syn-thesized inhibitor is nontoxic to cultured cells at a high concentration and to mouse at a high dose. The syn-thetic procedure developed here can be used to produce a large amount of the high-quality CFTRinh-72 suitablefor antidiarrheal studies and for creation of cystic fibrosis models in large animals. The procedure can be usedto synthesize radiolabled CFTRinh-72 for in νiνo pharmacokinetics studies.