高等数学的整体教学法

本文首先探讨了高等数学的整体结构的三个层次即宏观、中观、微观层次 ,并绘制了多个相应于 [5]的各种关系结构图 ,其次给出了整体教学法及其三个子模式和实施步骤 ,还给出了笔者具体实施的作法     

第29届奥运会中外男篮技术成绩统计分析

本文对第29届奥运会中国男篮同相遇对手的技术成绩指标,进行了相关性、偏相关、回归分析等多元统计分析,通过回归方程找出影响总得分的主要因素,利用多因变量的线性模型对29届中国队与对手整体上进行比较,并对中国队在28、29两届上进行了整体的比较。结果显示:29届奥运会上中国队和对手在整体水平上有一定的差异;和28届相比,中国队前后差异不大,说明中国男篮进步幅度不是太大。     

考虑土-结构相互作用效应的三维桩基结构动力有限元分析

结合简化阻抗法建立了三维相互作用结构的动力有限元分析模型,导出了考虑群桩刚体、惯性效应作用时的结构相互作用时程积分方程式,以近乎纯结构有限元的建模途径合理地反映出桩-土-上部结构在水平地震作用下的动力相互作用特性。模型中引入具有桩(筏)-土阻抗特性的弹阻单元来描述不同群桩布置、土层状况因素对体系反应的参与作用,而在动力方程中竖向SV剪切波经桩土刚体相互作用产生的水平、摇摆分量对体系的影响亦得以体现。20层桩承刚框架结构的动力分析表明:较柔的桩、土基础使得体系的SSI效应增强,但其参与程度与群桩效应相关,而结构构件内力较不考虑相互作用时有较大折减;应用该法可精确、快速地进行复杂上、下部结构时程反应分析。     

局部与整体水压法在水下饱和边坡稳定分析中的应用

针对以考虑土条侧向条间力来提高瑞典条分法的计算精度方法, 作者基于物理学思想, 提出了局部与整体水压法以进行水下饱和边坡稳定分析, 并采用该法对瑞典条分法中一对相互矛盾的"真假李逵" 式进行了辨析. 指出李鬼式精度低的原因在于直接提取土条顶面和底面水压力沿着竖向的分量来建立平衡, 而忽略了其沿着水平方向分力的影响, 并证明了局部与整体水压法在提高有水边坡计算精度和便捷度时具有的普适性.     

固相支持液-液萃取/液相色谱-串联质谱法测定人全血中环孢素A

采用固相支持液-液萃取/液相色谱-串联质谱(LC-MS/MS)技术建立了人全血中环孢素A的分析方法。全血样品经蛋白沉淀后,上样至固相支持液-液萃取柱,经甲基叔丁基醚洗脱,Shim-pack XR-ODS色谱柱(75 mm×3. 0 mm i. d.,2. 2μm)分离,电喷雾电离源、正离子模式和多反应监测模式下采集数据,以环孢素D为内标物定量。结果表明,环孢素A在1. 5～500μg/L范围内呈良好的线性关系,相关系数(r)为0. 998,检出限和定量下限分别为0. 5μg/L和1. 5μg/L,在50、100、400μg/L 3个加标浓度下的平均回收率为78. 6%～83. 5%,日内和日间相对标准偏差(n=3)分别为3. 1%～5. 6%和4. 5%～8. 3%。该方法操作简便、灵敏度高,可用于全血中环孢素A的分析。     

Application of a highly specific and sensitive fluorescent HPLC method for topotecan lactone in whole blood

Individualization of topotecan dosing reduces inter‐patient variability in topotecan exposure, presumably reducing toxicity and increasing efficacy. However, logistical limitations (e.g. requirement for plasma, intensive bedside plasma processing) have prevented widespread application of this approach to dosing topotecan. Thus, the objectives of the present study were to develop and validate an HPLC with fluorescence detection method to measure topotecan lactone in whole blood samples and to evaluate its application to individualizing topotecan dosing. Plasma samples (200 µL) were prepared using methanolic precipitation, a filtration step and then injection of 100 µL of the methanolic extract onto a Novapak® C₁₈, 4 µm, 3.9 × 150 mm column with an isocratic mobile phase. Analytes were detected with a Shimadzu Fluorescence RF‐10AXL detector with an excitation and emission wavelength of 370 and 520 nm, respectively. This method had a lower limit of quantification of 1 ng/mL (S/N ≥ 5; RSD 4.9%) and was validated over a linear range of 1–100 ng/mL. Results from a 5‐day validation study demonstrated good within‐day and between‐day precision and accuracy. Data are presented to demonstrate that the present method can be used with whole blood samples to individualize topotecan dosing in children with cancer. Copyright © 2009 John Wiley & Sons, Ltd.     

动态微分电位溶出法直接测定微量全血中铅的研究

简述了电化学方法测铅的发展及优越性,重点给出了一种简便可靠的直接测定微量全血中铅的方法。该法检测下限可达0.2ng/mL,线性范围0-0.04μg/50μL血,相关系数r=0.9991,相对标准偏差6.3%-22%,平均加标回收率83.9%-117.6%。实验结果表明,该法能够有效排除血中有机成分的干扰、快速、灵敏地测定微量全血中铅的含量,且重现性较好,巳能基本满足常规临床检验的需要。     

Comparative Biochemistry of the Physiologically Active Components of Venom, Hemolymph, and Eggs of the Karakurt Spider (Latrodectus tredecimguttatus)

The physicochemical and functional properties of the venom, hemolymph, and egg extract of the karakurt spiderLatrodectus tredecimguttatuswere studied. A highly purified toxin that differs from the neurotoxins found previously in the venom was isolated from the hemolymph and characterized.     

Application of immobilized hydrogenase to h2 storage in concentrated solutions of methyl viologen

It has been found that immobilized cells ofC. pasteurianum possessing hydrogenase activity efficiently catalyze reversible reduction of concentrated (up to 0.5M) solutions of methyl viologen with H₂. A 0.5M aqueous solution of methyl viologen dissolves 240 times as much H₂ as pure water under the same pressure of hydrogen. The experimentally obtained levels of methyl viologen reduction and H₂ evolution are in satisfactory agreement with theoretical calculations. The potential of concentrated solutions of methyl viologen containing immobilized hydrogenase as a H₂ storage medium is discussed.     

Simultaneous determination of ten amphetamine designer drugs in human whole blood by capillary electrophoresis with diode array detection

In recent years, a number of new designer drugs have entered the illicit drug market. The methylenedioxyderivatives of amphetamine represent the largest group of designer drugs. This paper describes a method for screening for and simultaneously quantifying 10 2,5-methylenedioxy-derivatives of amphetamine and phenethylamine in human whole blood, using capillary electrophoresis (CE) with diode array detection (DAD). Using an aqueous pH 2.5 phosphate buffer, CE analysis gave peaks with good symmetry and reproducible migration times. Under these experimental conditions, the 10 amphetamines were resolved in 15 min and without interference from biological matrices (blood). Their identification by migration time was confirmed by their UV spectra recorded with a DAD (190-350 nm). The main advantages of the present method lie in its simplicity, clean and reliable extraction from human whole blood and simultaneous detection and quantification by CE-DAD. The applicability of the method was demonstrated by analysis of in vivo rat blood samples. The method was validated according to international guidelines.