高效液相色谱-荧光检测法同时测定全血中5种香豆素类杀鼠剂

建立了同时测定全血中杀鼠灵、杀鼠迷、溴敌隆、氟鼠灵与溴鼠灵等5种香豆素类杀鼠剂的简便、灵敏、准确的高效液相色谱-变波长荧光分析方法。全血样品经乙酸乙酯提取后,在XDB C18柱(150 mm×2.1 mm, 5 μm)上以甲醇-0.2%乙酸水溶液(体积比为88∶12)混合液为流动相,采用荧光变波长程序检测,同时通过建立各杀鼠剂的荧光光谱库,利用谱库检索,提高定性的准确度。各杀鼠剂的线性范围为0.01~10.00 mg/L（杀鼠灵为0.05~10.00 mg/L）,检出限为0.01~0.05 mg/L,方法的加标回收率为81%～98%,其相对标准偏差（RSD）为3.8%～8.5%。该法适用于中毒病人的中毒诊断检测。     

全谱拟合法研究聚丙烯腈基碳纤维形成过程中晶态结构演变

通过全谱拟合法对碳纤维制备过程中不同阶段纤维的XRD谱图进行处理,得到不同阶段纤维的微观结构参数,研究了聚丙烯腈(PAN)基碳纤维制备过程中晶态结构的演变.全谱拟合法基于晶体衍射的严格物理理论,拟合目标为整个衍射谱,并不是个别衍射峰,所得结果具有更高的可信度.研究结果表明:PAN原丝中的高分子链沿纤维轴高度取向,表观晶粒尺寸为6.5 nm左右;经过预氧化处理,纤维中的有序结构遭到破坏,表观晶粒尺寸锐减.纤维中逐渐形成梯形结构并沿纤维轴取向,从而形成新的有序结构;经过碳化处理后,环状梯形结构转变为碳的层状结 关键词： 碳纤维 晶体结构 XRD 全谱拟合法     

40J激光装置研制

 报道了一台输出能量在40J量级的激光装置。该装置从星光Ⅱ激光装置分光作为光源,由放大器链、能源系统和支撑桁架等三大部分构成。对整个激光装置的模拟计算结果表明：装置的增益能力足够输出40J,B积分满足ΔB小于1.8的要求。研制了一种全腔水冷新型放大器,对其小信号增益系数和增益均匀性进行了测量,实验得出增益系数β为0.034cm ^-1,增益均匀性为91.84%。结果表明这种放大器完全能满足此激光装置的设计要求。     

一厂多商联盟的利润分享方案和整段返利方法的确定

供应链中厂家与商家是一种合作竞争关系.本文从一厂一商型联盟的最大利润化的销售问题入手,在追求厂商供应链利润最大化的前提下,建立了一厂多商型联盟的利润模型.基于该模型,确定了联盟及厂商均可增加利润的分享方案,提出了基于分享方案而实行非固定的整段返利的数学表达式,且对整段返利的机制进行了讨论.研究结论表明用这一方法建立的返利机制,可以保障厂商联盟利润分享方案的实施.     

Estimation of the possibility of using dynamic spectroscopy to analyze biological scattering media

An attempt was made to extend the field of application of the methods of dynamic spectroscopy to analyze media with a large volume content of scatterers. It is shown that under conditions of multiple scattering the temporal spectrum S(w) depends not only on the dimensions of the scatterers, but also on their optical characteristics. A correlation dependence between the width of the temporal spectrum S(w) and the dimensions of aggregates of erythrocytes has been established. The existence of a correlation between the width of the temporal spectrum S(w) and important clinical characteristics of blood, such as the erythrocyte settling rate and the concentration of lipoproteins, has been found. Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 64, No. 1, pp. 97–102, January–February, 1997.     

Liquid chromatographic assay for the cyclic depsipeptide aplidine, a new marine antitumor drug, in whole blood using derivatization with trans-4'-hydrazino-2-stilbazole

A sensitive bio-analytical assay for the depsipeptide aplidine in plasma has been modified and tested for human whole blood samples. The adapted method is based on reversed-phase liquid chromatography and fluorescence detection of the trans-4'-hydrazino-2-stilbazole derivative of the analyte. Aplidine is isolated from the matrix by solid-phase extraction on an octadecyl modified silica stationary phase. After evaporation of the acetone eluate, the derivatization with the hydrazino reagent is performed in a water-acetonitrile mixture at pH = 4. The reaction mixture is injected directly into the chromatograph and the analyte is quantified by fluorescence detection at 410 and 560 nm for excitation and emission, respectively. The method has been validated in the 2-100 ng/mL range, with 2 ng/mL being the lower limit of quantification. Precision and accuracy both meet the current requirements for a bioanalytical assay. The stability of aplidine in whole blood at ambient temperature and at 37 degrees C is limited; recoveries in the range 60-85% were observed after 7 h. Further, adequate stability of aplidine in plasma at -80 and -20 degrees C for 35 months could now be demonstrated.     

生物催化硫醚底物的不对称氧化反应研究进展

综述了生物催化硫醚底物的不对称氧化反应的研究进展,重点介绍了微生物菌株整细胞、纯酶和基因工程菌等催化剂在硫醚底物的不对称氧化反应中的应用。参考文献32篇。     

偏振旋转孔散斑照相法

文提出动态散斑技术中一种新的孔径:偏振旋转孔。在旋转孔径处适当布置偏振片能使衍射晕能量分布均匀,信息存储合理,散斑条纹清晰度提高。偏振转孔法能在一张散斑图上记录下全部动态过程,并连续地再现出来。本文介绍了偏振菱位孔,偏振平移孔,偏振四扇孔,论述了它们的全场分析和逐点分析功能,给出了理论分析和实验证明。     

基于广义等价理论的高保真共振计算方法

采用伪核素方法,建立了能够精确快速处理干涉效应的计算方法,在分析广义等价理论特点的基础上,提出了高保真的共振计算方法。该方法首先根据实际问题的几何,建立238U的非均匀共振积分表,基于此核素采用广义等价理论处理栅元间的互屏效应,获得不同位置燃料棒的背景截面,然后根据背景截面在非均匀截面表中插值建立不同燃料棒的等效栅元,在此栅元的基础上根据实际问题中燃料棒内的温度分布和核素分布进行超细群计算,从而获得燃料棒内不同位置共振核素高保真的有效共振截面。