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Arena G Fattorusso R Grasso G Grasso GI Isernia C Malgieri G Milardi D Rizzarelli E 《Chemistry (Weinheim an der Bergstrasse, Germany)》2011,17(41):11596-11603
Intraneuronal inclusions consisting of hypermetallated, (poly-)ubiquitinated proteins are a hallmark of neurodegeneration. To highlight the possible role played by metal ions in the dysfunction of the ubiquitin-proteasome system, here we report on zinc(II)/ubiquitin binding in terms of affinity constants, speciation, preferential binding sites and effects on protein stability and self-assembly. Potentiometric titrations allowed us to establish that at neutral pH only two species, ZnUb and Zn(2)Ub, are present in solution, in line with ESI-MS data. A change in the diffusion coefficient of ubiquitin was observed by NMR DOSY experiments after addition of Zn(II) ions, and thus indicates metal-promoted formation of protein assemblies. Analysis of (1)H, (15)N, (13)Cα and (13)CO chemical-shift perturbation after equimolar addition of Zn(II) ions to ubiquitin outlined two different metal-binding modes. The first involves a dynamic equilibrium in which zinc(II) is shared between a region including Met1, Gln2, Ile3, Phe4, Thr12, Leu15, Glu16, Val17, Glu18, Ile61 and Gln62 residues, which represent a site already described for copper binding, and a domain comprising Ile23, Glu24, Lys27, Ala28, Gln49, Glu51, Asp52, Arg54 and Thr55 residues. A second looser binding mode is centred on His68. Differential scanning calorimetry evidenced that addition of increasing amounts of Zn(II) ions does not affect protein thermal stability; rather it influences the shape of thermograms because of the increased propensity of ubiquitin to self-associate. The results presented here indicate that Zn(II) ions may interact with specific regions of ubiquitin and promote protein-protein contacts. 相似文献
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Cover Picture: Dissecting Ubiquitin Signaling with Linkage‐Defined and Protease Resistant Ubiquitin Chains (Angew. Chem. Int. Ed. 47/2014)
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Marcel Ottiger Frank Delaglio John L. Marquardt Nico Tjandra Ad Bax 《Journal of magnetic resonance (San Diego, Calif. : 1997)》1998,134(2):365-369
A simple and effective method is described for simultaneously measuring dipolar couplings for methine, methylene, and methyl groups in weakly oriented macromolecules. The method is aJ-modulated 3D version of the well-known [1H-13C] CT-HSQC experiment, from which theJand dipolar information are most accurately extracted by using time-domain fitting in the third, constant-time dimension. For CH2-sites, the method generally yields only the sum of the two individual13C-1H couplings. Structure calculations are carried out by minimizing the deviation between the measured sum, and the sum predicted for each methylene on the basis of the structure. For rapidly spinning methyl groups the dipolar contribution to the splitting of the outer13C quartet components can be used directly to constrain the orientation of the C-CH3bond. Measured sidechain dipolar couplings are in good agreement with an ensemble of NMR structures calculated without use of these couplings. 相似文献
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Small‐Molecule Control of Intracellular Protein Levels through Modulation of the Ubiquitin Proteasome System
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Dr. Dennis L. Buckley Prof. Craig M. Crews 《Angewandte Chemie (International ed. in English)》2014,53(9):2312-2330
Traditionally, biological probes and drugs have targeted the activities of proteins (such as enzymes and receptors) that can be readily controlled by small molecules. The remaining majority of the proteome has been deemed “undruggable”. By using small‐molecule modulators of the ubiquitin proteasome, protein levels, rather than protein activity, can be targeted instead, thus increasing the number of druggable targets. Whereas targeting of the proteasome itself can lead to a global increase in protein levels, the targeting of other components of the UPS (e.g., the E3 ubiquitin ligases) can lead to an increase in protein levels in a more targeted fashion. Alternatively, multiple strategies for inducing protein degradation with small‐molecule probes are emerging. With the ability to induce and inhibit the degradation of targeted proteins, small‐molecule modulators of the UPS have the potential to significantly expand the druggable portion of the proteome beyond traditional targets, such as enzymes and receptors. 相似文献
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Stanislav Falkovich Anatolii Darinskii Nikolai Balabaev Igor Neelov 《Macromolecular Symposia》2009,278(1):105-113
Mechanical extension of the ubiquitin with constant speed in five different directions is simulated on coarse-grained Go-like and all-atom models. The anisotropy of the mechanical resistance of the protein is observed in agreement with experimental data. Differences and similarities between the results obtained for two models are discussed. It is shown that the unfolding begins from the rupture of contacts between residues located in the vicinity of points of the external load application. 相似文献
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Ashton C. Lai Dr. Momar Toure Dr. Doris Hellerschmied Jemilat Salami Dr. Saul Jaime‐Figueroa Dr. Eunhwa Ko Dr. John Hines Prof. Craig M. Crews 《Angewandte Chemie (International ed. in English)》2016,55(2):807-810
Proteolysis Targeting Chimera (PROTAC) technology is a rapidly emerging alternative therapeutic strategy with the potential to address many of the challenges currently faced in modern drug development programs. PROTAC technology employs small molecules that recruit target proteins for ubiquitination and removal by the proteasome. The synthesis of PROTAC compounds that mediate the degradation of c‐ABL and BCR‐ABL by recruiting either Cereblon or Von Hippel Lindau E3 ligases is reported. During the course of their development, we discovered that the capacity of a PROTAC to induce degradation involves more than just target binding: the identity of the inhibitor warhead and the recruited E3 ligase largely determine the degradation profiles of the compounds; thus, as a starting point for PROTAC development, both the target ligand and the recruited E3 ligase should be varied to rapidly generate a PROTAC with the desired degradation profile. 相似文献