丹参超微粉碎前后丹参酮ⅡA的溶出行为比较

采用高效液相色谱法测定丹参超微粉体和常规粉体中丹参酮ⅡA体外溶出量和溶出速率.丹参超微粉体和常规粉体中丹参酮ⅡA体外溶出量无显著性差异,超微粉体的溶出速率较常规粉体明显增加.实验表明超微粉碎可明显提高丹参酮ⅡA溶出速率.     

Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobium huoshanense C.Z. Tang et S.J. Cheng

A water-soluble polysaccharide TC-DHPA4 with a molecular weight of 8.0 × 10⁵ Da was isolated from tissue-cultured Dendrobium huoshanense by anion exchange and gel permeation chromatography. Monosaccharide analysis revealed that the homogeneous polysaccharide was made up of rhamnose, arabinose, mannose, glucose, galactose and glucuronic acid with a molar ratio of 1.28:1:1.67:4.71:10.43:1.42. The sugar residue sequence analysis based on the GC-MS files and NMR spectra indicated that the backbone of TC-DHPA4 consisted of the repeated units:→6)-β-Galp-(1→6)-β-Galp-(1→4)-β-GlcpA-(1→6)-β-Glcp-(1→6)-β-Glcp-(→. The sugar residue sequences β-Glcp-(1→)-α-Rhap-(1→3)-β-Galp-(1→, β-Glcp-(1→4)-α-Rhap-(1→3)-β-Galp-(1→, β-Galp-(1→6)-β-Manp-(1→3)-β-Galp-(1→, and α-l-Araf-(1→2)-β-Manp-(1→3)-β-Galp-(1→ were identified as the branches attached to the C-3 position of (1→6)-linked galactose in the backbone.     

Plant Coumarins. 3. (+)-PTeryxin from <Emphasis Type="Italic">Peucedanum terebinthaceum</Emphasis>

The potentially medically valuable pyranocoumarin (+)-pteryxin was isolated for the first time from Peucedanum terebinthaceum Fischer et Turcz. The structure of (+)-pteryxin was rigorously proved using mass spectrometry, NMR, IR, and UV spectroscopy and comparison of the spectral characteristics of this compound and its basic hydrolysis products (+)-cis- and (−)-trans-khellactone and angelic and acetic acids. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 468–470, September-October, 2008.     

Chemical composition and nutritional properties of Terminalia catappa L. oil and kernels from Benin

This work focuses on the physico-chemical characterization of kernels and oil of Terminalia catappa L. from Benin. The detailed physico-chemical properties are given including the nutritional composition and fatty acid profile, but also the phenolic content, phytochemical screening and antioxidant capacity which were determined for the first time. The kernel (100 g) contained 5.5 g of moisture, a high level of lipids (64.7–140.4 of Recommended Daily Intake (RDI)), proteins (36.0% RDI), sugars (6.0% RDI), and tannins (0.6%). The defatted kernels (100 g) contained high levels of manganese (184.8–236.1% RDI), magnesium (173.6–235.2% RDI), iron (89.7–201.9% RDI), zinc (87.9–120.9% RDI) and calcium (41.5% RDI), and contributed for 98.6% of RDI proteins. The kernel oil showed a high level of unsaturated fatty acids including oleic (27.1%) and linoleic acids (26.6%) and saturated fatty acids such as palmitic acid (40.0%) as well as several phytosterols and triterpenes. These kernels and their unsaturated oil are of interesting nutritional value but could also be used as a biofuel or lubricant. The presence of phenolic and terpenic derivatives may also explain at least in part their use in traditional medicine.     

Application of liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry for screening and quantitative analysis of C21 steroids in the roots and rhizomes of Cynanchum paniculatum

A liquid chromatography-mass spectrometry method is presented for the quantification of C21 steroids in the roots and rhizomes of Cynanchum paniculatum. Eight C21 steroids, including five steroidal aglycones and three steroidal glycosides, were simultaneously analyzed by liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry. The extracted ion current chromatograms were extracted from the total ion current chromatogram using characteristic ions produced by target compounds for peak determination. Chromatographic separation was achieved on a C18 reversed-phase column within 60 min, using an acetonitrile/water gradient. For comparision, six C. paniculatum samples from different locations were investigated by the established method, and the results indicated that the different geographical origin significantly influenced the C21 steroid composition. The method was observed to have the necessary sensitivity, selectivity, precision, and accuracy, and to be suitable for quality control of herbal medicines and their preparations.     

环氧乙烷的真空紫外同步辐射光电离与光解离

利用真空紫外同步辐射和自制的飞行时间质谱（TOF－MS）仪,研究了环氧乙烷的光电离与光解离过程,通过测量各离子的光电高效率（PIE）曲线,获得了该分子的电离势和所有碎片离子的出现势．分析了离子的光电离解离通道,并讨论了它们的竞争情况．结合有关公认的热力学数据,算出它们的标准生成焓．     

基于均匀设计优化深山草莓花瓣离体培养及植株再生体系

应用均匀设计法对深山草莓花瓣诱导愈伤组织和愈伤组织再分化芽苗的主要因子和水平进行了探讨.结果表明,深山草莓花瓣愈伤组织诱导的适宜培养基为LS+TDZ 2.06 mg.L-1+2,4-D 0.60 mg.L-1,诱导率为98%;愈伤组织再分化芽苗的适宜培养基为1/2 LS+TDZ 2.30 mg.L-1+NAA 0.10 mg.L-1+KT1.30 mg.L-1,分化率为96.3%.炼苗移栽后观察植株的形态、花、果及生长等特征,以筛选具有优良性状的株系.     

Chemical composition and antioxidant activity of Borago officinalis L. leaf extract growing in Algeria

The aqueous and hydroalcoholic extracts of borage (Borago officinalis) leaves from Annaba region (Algeria) were preliminary analyzed for their phenolic profile (total phenolics, total flavonoids, total flavonols, total tannins and total anthocyanins). These extracts were evaluated for their antioxidant properties by different methods such as DPPH radical scavenging, test NBT and total antioxidant activity. The two extracts have exhibited a high antiradical capacity. Indeed, the ethanolic extract showed the lower IC50 values and the highest amount of phenolics (94.09 ± 1.72 mg gallic acid/g dry extract). Using LC-MS/MS analysis, it was possible to identify phenolic acids, flavonoids, sterol and for the first time oleuropein was identified in the aqueous extract of the plant. The obtained results have demonstrated that phenolic compounds are the major contributor to the antioxidant activity of plants.     

IR光谱分析PEO/V2O5纳米复合材料结构

聚合物溶液直接嵌入法制备 PEO/ V2 O5纳米复合材料。由红外光谱 (IR)表征了 PEO与氧化物层之间的相互作用。V2 O5干凝胶的钒氧双键 (V=O)主要与层间聚合物 PEO中的醚氧键 (Oet)发生作用 ,以Oet…V=O形式存在     

树脂法分离纯化倒提壶生物碱的研究

采用阳离子交换树脂和大孔吸附树脂联用的方法,将倒提壶生物碱提取液依次经过阳离子交换树脂和两次大孔吸附树脂,对其中的天芥菜碱和毛果天芥菜碱进行分离纯化。结果表明,倒提壶生物碱溶液采用阳离子交换树脂经过水洗和NaCl洗脱与大孔吸附树脂两次经乙醚和50%乙醇洗脱后,毛果天芥菜碱与天芥菜碱的纯度分别从0.73%和2.2%提高至81.6%和79.2%,提取量分别由4.6 mg/g和10.7 mg/g提高至50.6mg/g和92.7 mg/g。该文所建立的分离纯化方法具有成本低、污染小、周期短、操作简便和分离纯化效率高等特点,适合工业化生产。