Preparation,evaluation and characterization of quercetin-molecularly imprinted polymer for preconcentration and clean-up of catechins

Molecularly imprinted polymer (MIP) for solid extraction and preconcentration of catechins have been successfully prepared by a thermal polymerization method using quercetin as template, 4-vinylpyridine as functional monomer and ethylene glycol dimethacrylate as crosslinker. A solution mixture of acetone and acetonitrile was used as porogen. Systematic investigations of the influence of monomer, cross-linker, porogen, as well as polymerization conditions on the properties of the MIPs were carried out. The quercetin MIPs were evaluated according to their selective recognition properties for quercetin, structurally related compounds (catechin, epigallocatechin gallate and epicatechin) and a unrelated compound of similar molecular size (α-tocopherol). Good binding was observed for quercetin, catechin and epigallocatechin gallate with an optimized MIP in a solid phase extraction system. Adsorption and kinetic characteristics were evaluated for catechins which indicated that the synthesized polymer had high adsorption capacity and contained homogeneous binding sites. Chemical and morphological characterization of the MIP was investigated by FTIR, SEM and BET, which confirmed a high degree of polymerization. Finally, the MIP was successfully applied to the clean-up and preconcentration of catechins from several natural samples.     

Ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence method for the detection of protein

An ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence (IM-ECL) method for the detection of protein is developed. The target protein is captured by biotin-labeled aptamer (biotin probe) and [Ru(bpy)₃]²⁺ (TBR)-Au bio bar code-labeled aptamer (ECL nanoprobe), to form a double aptamer–protein sandwich complex. The complex is then immobilized on the streptavidin microbeads through biotin–streptavidin linkage and detected by ECL assay. The ECL signal of the target protein is amplified by the TBR-bio bar code DNAs. As an example, platelet-derived growth factor B-chain homodimer (PDGF-BB) was detected by the method. Experimental results show that the detection limit of the assay is 1 pM of PDGF-BB. A calibration curve with a linearity range from 1 pM to 10 nM is established, thus, make quantitative analysis possible. The method has been used to detect PDGF-BB in fetal calf serum with minimum background interference. Due to the wide availability of aptamer for numerous proteins, this aptamer-based bio bar code IM-ECL method holds great promise in protein detection.     

Generalized boublick equation: an accurate expression for the equation of state of hard fused spheres

Abstract

A simple expression to calculate the shape factor of hard bodies is proposed. Introducing this factor in the Boublik equation of state, very good results are obtained for hard dumbells and more complicated systems of linear homonuclear hard fused spheres. Agreement with available Monte Carlo results are also satisfactory enough for heteronuclear molecules. Furthermore, the new expression is reduced to the classical shape factor for hard convex bodies and provides a common basis to manage to concave and convex hard bodies.     

Maximum Δ‐edge‐colorable subgraphs of class II graphs

A graph G is class II, if its chromatic index is at least Δ + 1. Let H be a maximum Δ‐edge‐colorable subgraph of G. The paper proves best possible lower bounds for |E(H)|/|E(G)|, and structural properties of maximum Δ‐edge‐colorable subgraphs. It is shown that every set of vertex‐disjoint cycles of a class II graph with Δ≥3 can be extended to a maximum Δ‐edge‐colorable subgraph. Simple graphs have a maximum Δ‐edge‐colorable subgraph such that the complement is a matching. Furthermore, a maximum Δ‐edge‐colorable subgraph of a simple graph is always class I. © 2011 Wiley Periodicals, Inc. J Graph Theory     

Proteomic analysis reveals platelet factor 4 and beta-thromboglobulin as prognostic markers in severe acute respiratory syndrome

Previously, we reported that proteomic fingerprints were present in sera of patients with severe acute respiratory syndrome (SARS), and could separate patients into subgroups with different prognoses. In the present study, we examined the prognostic values of the SARS-associated proteomic features by biostatistical analysis, and deciphered the identities of those with prognostic values. Data of 20 SARS-associated serum proteomic features and ten serological variables from 38 SARS adult patients before treatment were subjected to multivariate logistic regression. Proteomic features of m/z 6634, m/z 7769, m/z 8635, and m/z 8865 were identified as independent prognostic markers. After purification by cation-exchange chromatography and gel electrophoresis, proteomic features of m/z 7769 and m/z 8865 were found to be platelet factor 4 (PF4) and beta-thromboglobulin (beta-TG) by tandem mass spectrometry, respectively. The associations of decreased serum PF4 and increased serum beta-TG levels with poor prognosis were confirmed by Western blot. Previous studies suggest that PF4 and beta-TG are involved in the pathogenesis of acute respiratory distress syndrome (ARDS) in a negative and positive way, respectively. Our results suggest that PF4 and beta-TG may also play similar roles in the development of ARDS in SARS patients.     

手性药物(S)-布洛芬氢键自组装印迹聚合物识别机理

以含有单一结合基团的手性药物(S)-布洛芬作为模板分子,制备了系列印迹聚合物.采用紫外-可见光谱和红外光谱对印迹及识别机理进行了研究.结果表明,模板分子与功能单体分别通过形成蓝移氢键和红移氢键完成预组装过程和再识别吸附过程,且形成了主客体配比为1∶1的配合物.等温吸附实验结果表明,印迹聚合物对模板分子表现出明显的选择性吸附,特异性吸附容量为37.92μmol/g,印迹指数为3.06,且印迹聚合物内特定的三维空间结构对其特异性吸附性能具有显著影响.由手性分离实验考察了印迹聚合物的拆分性能,其对(R)-布洛芬的分离因子为1.79.     

基于区间数据分析的各学科期刊发展现状研究

由于学科的自身特点和统计源的学科结构特点,不同学科期刊具有显著差异。但由于期刊数量庞大,评价指标多种多样,很难从宏观上把握各学科期刊的整体特点,对学科间差异给出科学的判断。本文以SCI期刊为研究对象,用区间数据代表各学科期刊,采用因素区间数据PCA算法探寻反映期刊水平的关键指标,并利用区间数据投影的方式绘制主平面图,反映各学科期刊的整体特点及我国期刊在国际上的地位。     

居民消费的习惯形成与实际经济周期模型的求解分析——一个基于三部门RBC模型的研究

结合H-P滤波法和King,Plosser&.Robelo(1987)的研究,探讨了一个求解引入居民消费的习惯形成和存在稳态趋势增长的RBC模型的对数线性化方法,并利用该方法求解引入习惯形成和政府支出冲击的三部门RBC模型来分析中国1979-2009年间宏观经济波动.研究表明:这个方法求解本文模型的预测结果与中国的特征事实较一致;与NHG方法求解的预测结果相比较,二者存在明显的差异;对中国经济的解释力要强于NHG方法求解的预测结果.     

关于广义鞍点问题的HSS迭代方法的收缩因子(英文)

白中治等提出了解非埃尔米特正定线性方程组的埃尔米特和反埃尔米特分裂(HSS)迭代方法(Bai Z Z,Golub G H,Ng M K.Hermitian and skew-Hermitian splitting methodsfor non-Hermitian positive definite linear systems.SIAM J.Matrix Anal.Appl.,2003,24:603-626).本文精确地估计了用HSS迭代方法求解广义鞍点问题时在加权2-范数和2-范数下的收缩因子.在实际的计算中,正是这些收缩因子而不是迭代矩阵的谱半径,本质上控制着HSS迭代方法的实际收敛速度.根据文中的分析,求解广义鞍点问题的HSS迭代方法的收缩因子在加权2-范数下等于1,在2-范数下它会大于等于1,而在某种适当选取的范数之下,它则会小于1.最后,用数值算例说明了理论结果的正确性.