Recent advances in 3D SEM surface reconstruction

The scanning electron microscope (SEM), as one of the most commonly used instruments in biology and material sciences, employs electrons instead of light to determine the surface properties of specimens. However, the SEM micrographs still remain 2D images. To effectively measure and visualize the surface attributes, we need to restore the 3D shape model from the SEM images. 3D surface reconstruction is a longstanding topic in microscopy vision as it offers quantitative and visual information for a variety of applications consisting medicine, pharmacology, chemistry, and mechanics. In this paper, we attempt to explain the expanding body of the work in this area, including a discussion of recent techniques and algorithms. With the present work, we also enhance the reliability, accuracy, and speed of 3D SEM surface reconstruction by designing and developing an optimized multi-view framework. We then consider several real-world experiments as well as synthetic data to examine the qualitative and quantitative attributes of our proposed framework. Furthermore, we present a taxonomy of 3D SEM surface reconstruction approaches and address several challenging issues as part of our future work.     

磁力显微镜对相分离锰氧化物薄膜中反铁磁绝缘相融化的成像

用自制磁力显微镜研究了一个受各向异性应变的锰氧化物薄膜中的相分离以及由磁场导致的从反铁磁绝缘相到铁磁金属相的转变．磁力显微镜图片显示,在0 T这两种竞争的相就已经共存,且两种相 的畴呈非常明显的各向异性的条状分布,这可以定性解释输运上的各向异性．在2.1 T 以上,反铁磁绝缘相逐渐转变为铁磁金属相,并在3.2 T时结束．当去掉磁场时铁磁金属相能够保持．     

Application of magnetic and core–shell nanoparticles to determine enrofloxacin and its metabolite using laser induced fluorescence microscope

A unique analytical method using nanoparticles and laser-induced fluorescence microscopy (LIFM) was developed to determine enrofloxacin in this work. For sample pretreatment, two different kinds of particles, i.e., synthesized dye-doped core–shell silica nanoparticles and magnetic micro-particles (MPs), were used for fluorescent tagging and concentrating the enrofloxacin, respectively. The antibody of enrofloxacin was immobilized on the synthesized FITC-doped core–shell nanoparticles, and the enrofloxacin target was extracted by the MPs. At this moment, the average number of antibodies on each core–shell silica nanoparticle was ∼0.9, which was determined by the fluorescence ratiometric method. The described method was demonstrated for a meat sample to determine enrofloxacin using LIFM, and the result was compared with enzyme-linked immunosorbent assay (ELISA). The developed technique allowed the simplified analytical procedure, improved the detection limit about 54-fold compared to ELISA.     

Nachweis vonα-Strahlung durch Festkörperspurdetektoren im Elektronenmikroskop

Einführung

Sehwere, geladene Partikel sind in der Lage, bci Wechselwirkung min dielektrischem Material in diesem nachweisbare Veränderungen zu hinterlassen. Steht eine strahlendeo Substanz mit einem solchen Material in Kontakt, so kann diese anhand der Verteilung der strahlenbedingten Spuren in dem Material lokalisiert werden. So ist es möglich, α-Strahlung in Polykarbonat, Cellulosenitrat oder Celhiloseazetat nach alkalischer Ätzung derPolymeren nachzuwcisen. Der Spurennachweis erfolgte bisher lichtmikroskopisch [1] oder im Rasterclektronenmikroskop [2].     

纳米二苯甲酰甲烷铕配合物的制备和表征

以聚乙烯吡咯烷酮为表面活性剂,在无水乙醇中制备了纳米二苯甲酰甲烷铕配合物Eu(DBM)3,对其进行了红外光谱(IR)、透射电镜(TEM)、X-射线衍射(XRD)、荧光光谱(FS)等表征。结果表明,配合物在聚乙烯吡咯烷酮中呈纳米颗粒分散,分散尺度在5～30Nm之间,与普通二苯甲酰甲烷铕配合物相比,其主要荧光发射峰强度较高;后者在675．6、702．6、719．3、740．5nm处的小峰消失,612．9nm峰红移至611．8nm。     

硫化铜精矿物相分析及氧化机理研究

在海轮运输及样品的制备过程中,进口硫化铜精矿氧化的现象普遍存在,使得货物的重量和铜品位发生变化,但该种现象的机理研究不多。针对硫化铜精矿的物相进行分析,并对硫化铜精矿的氧化机理进行研究。首先,利用显微镜、扫描电子显微镜、X射线荧光光谱(XRF)、X射线衍射(XRD)等方法对两个硫化铜精矿进行物相分析,并对其中的主要元素和含铜主要物相进行定量化学分析。再次,利用热重分析(TGA)研究这两个硫化铜精矿发生氧化反应的温度,结果表明在400℃时铜精矿样品重量开始增加,在约650℃重量达到顶峰,随后重量开始下降。最后,分别在400、650和900℃对两个铜精矿样品进行灼烧,并利用XRF和XRD方法进行测定,从而研究铜精矿的氧化机理,结果表明在400℃时,出现了铁的氧化物和铜的硫氧化物,说明黄铜矿和黄铁矿开始氧化,但还没有完全氧化,在650℃时,化合物中的氧含量继续增加,硫含量逐步减少,在900℃时,铜和铁的化合物只剩氧化物,硫已全部被氧化。结果表明,硫化铜精矿中的铜、铁等元素在温度和时间的作用下逐步氧化,硫氧化物的产生为中间态,氧化物的产生为最终态。实验解释了进口硫化铜精矿氧化的原理,有助于贸易双方减少分歧,有助于海关技术中心更规范地制备样品,降低数据误差。     

对半导体杂质沉淀无损检测的若干结果

用XCD-H红外电视测微显微镜,通过无损检测来评价半导体材料与器件工艺的质量。本文仅对材料的完整性与芯片制造工艺导致杂质沉淀的问题进行讨论。     

发光多孔硅表面的STM研究

自从Canham首次报道了室温下多孔硅的光致发光现象以来^[1],多孔硅已成为半导体光电化学及材料领域内最为热门的研究课题^[2].     

Ordering of Colloidal Spheres Floating on the Surface of Glycol Film

The ordering of polystyrene colloidal particles floating on the surface of glycol film, forming a two-dimensional system,was studied via an optical microscope. We have observed that the interfacial colloidal particles crystallized to form a two-dimensional triangular lattice with lots of point defects and grain boundaries. The interactions between the interfacial colloidal particles are also analyzed.     

Resolution deterioration in emission electron microscopy due to object roughness

In the present paper a general analytic expression has been obtained and confirmed by a computer simulation which links the surface roughness of an object under study in an emission electron microscope and it's resolution. A quantitative derivation was made for the model case when there is a step on the object surface. It was shown that the resolution is deteriorated asymmetrically relative to the step. The effect sets a practical limit to the ultimate lateral resolution obtainable in an emission electron microscope.