Real‐Time Nanomicroscopy via Three‐Dimensional Single‐Particle Tracking

We developed a new method for real‐time, three‐dimensional tracking of fluorescent particles. The instrument is based on a laser‐scanning confocal microscope where the focus of the laser beam is scanned or orbited around the particle. Two confocal pinholes are used to simultaneously monitor regions immediately above and below the particle and a feedback loop is used to keep the orbit centered on the particle. For moderate count rates, this system can track particles with 15 nm spatial resolution in the lateral dimensions and 50 nm in the axial dimension at a temporal resolution of 32 ms. To investigate the interaction of the tracked particles with cellular components, we have combined our orbital tracking microscope with a dual‐color, wide‐field setup. Dual‐color fluorescence wide‐field images are recorded simultaneously in the same image plane as the particle being tracked. The functionality of the system was demonstrated by tracking fluorescent‐labeled artificial viruses in tubulin‐eGFP expressing HUH7 cells. The resulting trajectories can be used to investigate the microtubule network with super resolution.     

用于彩色目标跟踪的改进粒子群优化算法

提出一种用于彩色目标跟踪的改进粒子群优化算法(Improved Particle Swarm Optimization Algorithms,IP-SOA)。针对彩色目标,选择加权彩色直方图作为目标的特征,选用Bhattacharyya系数作为特征相似性度量,其最大值位置表示目标位置。对粒子群优化算法进行了改进,即自动调整惯性权重函数与认知学习因子,每次递推时对粒子速度、单帧位移总量加以限制,对Bhattacharyya系数优化,快速求取函数最大值位置。利用彩色序列图像进行仿真实验,结果表明,该方法能够实时跟踪飞机、车辆等目标,在目标被部分遮挡时能稳健跟踪。     

一种基于算法融合的红外目标跟踪方法

视频目标跟踪的难点在于快速、准确地在帧与帧之间匹配目标.由于红外图像目标与背景的反差低,图像的边缘模糊并且灰度级动态范围小,使红外目标跟踪难度比可见光更大.本文提出一种针对红外日标跟踪的融合算法,该方法融合直方图和不变矩的特点.首先利用目标的直方图计算简单快速的特点,由均值平移算法快速找到局部最优解,但由于该局部最优解仪为直方图匹配的最优解,缺少目标形状特征,与实际目标位置存在一定的偏差;其次,利用边缘小变矩作为修正特征修正误差,避免跟踪误差逐渐累计而最终导致跟踪失败,以提高跟踪的稳定性和精度.实验结果表明,该算法能够消除跟踪过程中的漂移现象,提高跟踪精度.     

应用阵面追踪法对散心爆轰波传播的数值模拟

考虑到爆轰波阵面曲率及化学反应区宽度的作用 ,由修正Hugoniot关系式解析求解了波后状态 ,应用阵面追踪法 (FTM)数值模拟了曲面散心爆轰波的传播。计算给出的散心爆轰波走时及波阵面上物理量都达到了波阵面曲率的一阶精度。     

The Reaction Activity of Aromatic Carbonyl Compounds with Diphenylphosphine Oxide Studied by 31P NMR

Abstract

A series of α-hydroxyphosphine oxides were prepared by the reactions of diphenylphosphine oxide and aromatic carbonyl compounds and characterized by ¹H NMR, ¹³C NMR, ³¹P NMR, FT-IR, ESI-MS, and HR-MS spectra. The reaction rates and experimental conditions of aromatic aldehydes and aromatic ketones were obviously different due to the activity of their carbonyls. The different substituents of the aromatic aldehydes affected the reaction rate too, and the quantitative reactivity of their substituent conformed to the Hammett equation. The results were confirmed by ³¹P NMR spectroscopy.     

五像限法太阳自动跟踪仪

介绍由两心圆组成的等面积五像限光测量装置工作原理，即当光线与光电池垂直时其投影光斑经过一透镜后正好安全覆盖五片光电池，当光线与光电池成一夹角时，其投影光斑必然发生偏移，将光主在光电池上的偏移转移成光电流差值的变化，实现装置自动跟踪太阳。     

Tracking control of high-performance robots via stabilizing controllers for uncertain systems

The development of flexible manufacturing systems calls for industrial robots characterized by robustness of performance with regard to the variations of the loads and real time specification of the trajectory in the work space. In this paper, the design of a feedback controller guaranteeing such performance is considered. At first, the manipulator dynamics are embedded into a larger class of uncertain dynamical systems and a class of feedback controls is proposed that guarantees uniform ultimate boundedness of the tracking error. Successively, the methodology is specialized for the case of robotic manipulators to track trajectories described in task-oriented coordinates; the proposed control algorithm operates without requiring any explicit coordinate transformation.     

五象限法声源自动跟踪器

介绍由两同心圆组成的等投影面积五象限声－电测量装置的工作原理。当声波与声－电转换器的主要测象限垂直时，其声波在主要测象限内产生的声－电电压最强。根据声－电电压的变化大小来确定声源的方向，实现自动跟踪。     

Colloidal particle motion as a diagnostic of DNA conformational transitions

Tethered particle motion is an experimental technique to monitor conformational changes in single molecules of DNA in real time, by observing the position fluctuations of a micrometer-size particle attached to the DNA. This article reviews some recent work on theoretical problems inherent in the interpretation of TPM experiments, both in equilibrium and dynamical aspects.