Enzymatic transformation of platycosides and one‐step separation of platycodin D by high‐speed countercurrent chromatography

Platycosides, the saponins found in the roots of Platycodon grandiflorum (Platycodi Radix), are typically composed of oleanane triterpenes with two side chains. In platycosides, platycodin D, a glucose unit at C‐3, is a major component, which has several pharmacological activities. Because of the high demand for this compound, we attempted to enzymatically convert platycodin D₃ and platycoside E, having two and three glucose units at C‐3, respectively, into platycodin D. In this study, we tested the ability of several glycosidases to transform platycosides, or more specifically, the ability to transform platycoside E and platycodin D₃ into platycodin D. To obtain pure platycodin D on a preparative scale, high‐speed countercurrent chromatography with a solvent system of ethyl acetate/n‐butanol/water (1.2:1:2, v/v/v) was used for the separation of the enzymatically transformed product. Approximately 39.4 mg of platycodin D (99.8% purity) was obtained from 200 mg of the product in a one‐step separation. The results strongly support the advantage of enzymatic transformation of the platycosides for the efficient enrichment of platycodin D in the complicated extract of the medicinal plant.     

Efficient isolation and purification of five products from microbial biotransformation of cinobufagin by high‐speed counter‐current chromatography

An efficient separation method of using high‐speed counter‐current chromatography was successfully established to directly purify cytotoxic transformed products of cinobufagin by Cordyceps militaris. The two‐phase solvent system composed of n‐hexane–ethyl acetate–methanol–water (4:6:3:4, v/v) was used in high‐speed counter‐current chromatography. A total of 9 mg of 4β,12α‐dihydroxyl‐cinobufagin ( 1 ), 15 mg of 12β‐hydroxyl‐cinobufagin ( 2 ), 8 mg of 5β‐hydroxyl‐cinobufagin ( 3 ), 12 mg of deacetylcinobufagin ( 4 ) and 6 mg of 3‐keto‐cinobufagin ( 5 ) were obtained in a one‐step separation from 400 mg of the crude extract with purity of 98.7, 97.2, 90.6, 99.1 and 99.4%, respectively, as determined by HPLC. Their chemical structures were identified on the basis of ¹H‐NMR and ¹³C‐NMR technology. All products ( 1 – 5 ) showed the potent activities against human carcinoma cervicis (Hela) and malignant melanoma (A375) cells in vitro.     

应用级联倍频方法提高倍频系统输出稳定性研究

提出应用级联倍频方法提高倍频系统输出稳定性,并就该方法的有效性进行了理论分析和模拟计算.分析和计算结果不但证明级联倍频方法能实现倍频系统稳定输出,而且还表明可以通过仔细调节第一块倍频晶体中波矢方向 k 与光轴间夹角、两块倍频晶体间的间隔,能调节改变实现倍频系统最稳输出时所需第二块晶体的理论计算长度,使之与第二块倍频晶体的实际加工长度一致,最终实现系统稳定倍频输出.级联倍频方法在实现高输出稳定性的同时能实现高的倍频转换效率,对应用于光参量啁啾脉冲放大系统的高稳定抽运源系统的设计建造具有重要参考意义. 关键词： 级联倍频 稳定倍频输出 光参量啁啾脉冲放大     

对冲火焰流场的二维分布对层流火焰传播速度测量的影响

在φ=0.6～0.75的条件下,利用PⅣ测得对冲火焰的二维速度场,分析使用偏移中心线一定距离提取的轴线速度分布曲线所得到的层流火焰传播速度SOL与沿中心线提取的速度分布曲线得到的SOL之间的误差大小.实验结果表明,在弱火焰时对冲火焰速度场具有明显的二维效应.φ=0.6,偏离中心轴线5 mm时,相对误差达到33%;而φ=...     

风场影响下直接空冷系统热风回流率的空间分布特性

环境风场影响下,电站直接空冷系统热风回流加剧,传热性能恶化。掌握风场作用下直接空冷系统热风回流率的空间分布特性,可为空冷系统的设计和运行提供理论依据。以典型2×600 MW直接空冷电站为例,通过数值模拟获得了冷却空气流场和温度场,分别计算了空冷岛和各个空冷单元的热风回流率。计算结果表明,随环境风速增加,空冷岛热风回流率升高,并随风向发生显著变化,炉后来风时空冷岛热风回流最为严重。空冷单元热风回流率呈现空间分布特性,风场不同区域空冷单元热风回流存在显著差异,处于风场上游空冷单元通常具有更大的热风回流率。     

用脉冲光声法测量固体介质中声速

提出了一种利用脉冲光声技术测量固体介质中声速的方法,建立了由YAG激光器和超声探测器组成的实验系统,脉冲激光在固体表面产生脉冲超声波,通过测量脉冲声波在固体内多次反射后的出射信号及固体的厚度,即可算出固体介质中的声速.对黄铜及铝的测量结果表明,这是一种准确性较高的固体介质中声速测量方法.该测量方法可作为综合设计性物理实...     

Traveling wave solutions in a diffusive system with two preys and one predator

This paper is concerned with the traveling wave solutions in a diffusive system with two preys and one predator. By constructing upper and lower solutions, the existence of nontrivial traveling wave solutions is established. The asymptotic behavior of traveling wave solutions is also confirmed by combining the asymptotic spreading with the contracting rectangles. Applying the theory of asymptotic spreading, the nonexistence of traveling wave solutions is proved.     

交叉概率对改进遗传算法运算速度影响的测试与分析

在现有文献基础上,通过理论分析,提出了交叉概率应取1.通过四个测试函数的测试结果,表明交叉概率越大,运算速度越快,平均运行代数越少,验证了通过理论分析提出的交叉概率应取1的科学性和可行性.同时克服了交叉概率难以确定以及确定交叉概率时缺少理论依据的不足.     

基于剪枝信任度传播的手写体目标形状匹配方法

手写体识别中,目标形状的匹配是较为重要的工作.为了提高手写体目标形状的匹配速度,提出一种新的匹配方法.由于手写体目标形状的几何先验知识已知,并可以采用少量的参数进行表示,新方法采用参数化可变形模板匹配目标形状,确定其后验概率模型,并定义剪枝信任度空间,依据信任度传播算法的特性,首次将剪枝信任度传播算法应用于求解可变形模板与目标形状之间的最佳匹配.实验结果显示,在灰度图像中,对手写体目标形状的轮廓检测与定位速度显著提高.提出将剪枝信任度传播方法应用于手写体目标形状的匹配工作,能够使得目标形状填补空白,应用于相关性较为稀疏的图模型中.     

基于Gristensen算法的砂岩储层横波速度计算方法

由于横波测量成本高,大部分油田测井资料中仅有少部分横波数据,则依据常规测井资料准确构建横波曲线已成为关键.考虑到横波构建理论方法更多地依赖实验室分析资料,提出在Gristensen经验公式基础上,以研究区横波测量曲线为约束,通过优化反演确立公式系数是一种好的研究思路.优化方法实现中采用阻尼型高斯牛顿法,保障了多参数优化迭代的收敛性和精度.将研究方法用于卫星油田横波数据构建中,取得好的计算结果.