磁性微粒子化学发光免疫分析法测定人血清中雌三醇

利用化学发光免疫分析(chemiluminescence immunoassay, CLIA)高灵敏度和高特异性的特点, 将磁性微粒子应用于化学发光免疫分析中, 用两种不同的方法对人体血清内的雌三醇(E3)含量进行了测定. 磁性微粒子分别作为固相一抗包被材料和二抗分离剂参与反应. 两种方法检测雌三醇浓度的线形范围均为0.6～60 ng/mL. 其中, 固相一抗法的批内变异及批间变异系数分别小于11%和15%, 回收率为90%～116%, 健全性系数为0.9987. 二抗分离法的批内变异及批间变异系数分别小于8%和10%, 回收率为88%～118%, 健全性系数为0.9974. 两种方法分别与经典板式化学发光法对比, 检测人血清样本, 结果相关性较好, 且磁性微粒子法更为省时、简便, 适于推广应用.     

Graphene-tuned threshold gain to achieve optical pulling force on microparticle

We investigate optical force on a graphene-coated gain microparticle by adopting the Maxwell's stress tensor method.It is found that there exists a threshold gain in obtaining the Fano-profile optical force which indicates the reversal of optical pushing and pulling force. And giant pushing/pulling force can be achieved if the gain value of the material is in the proximity of the threshold gain. Our results show that the threshold gain is more sensitive to the relaxation time than to the Fermi energy of the graphene. We further study the optical force on larger microparticle to demonstrate the pulling force occurring at octupole resonance with small gain value and then it will appear at quadrupole resonance by increasing gain value. Our work provides an in-depth insight into the interaction between light and gain material and gives the additional degree of freedom to optical manipulation of microparticle.     

One‐pot synthesis of organic–inorganic hybrid polyhedral oligomeric silsesquioxane microparticles in a double‐zone temperature controlled microfluidic reactor

Polyhedral oligomeric silsesquioxane (POSS) particles are one of the smallest organosilica nano‐cage structures with high multifunctionality that show both organic and inorganic properties. Until now poly(POSS) structures have been synthesized from beginning with a methacryl‐POSS monomer in free‐radical mechanism with batch‐wise methods that use sacrificial templates or additional multisteps. This study introduces a novel one‐pot synthesis inside a continuous flow, double temperature zone microfluidic reactor where the methodology is based on dispersion polymerization. As a result, spherical monodisperse POSS microparticles were obtained and characterized to determine their morphology, surface chemical structure, and thermal behavior by SEM, FTIR, and TGA, respectively. These results were also compared and reported with the outcomes of batch‐wise synthesis. © 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 1396–1403     

Synthesis and characterization of permalloy nanostructured films by deposition of laser ablated nanoparticles

A permalloy (Ni₈₁Fe₁₉; at%) microparticle (MP) aerosol was ablated to produce a nanoparticle (NP) aerosol that was then impacted at high velocity onto a substrate to produce porous thick films. The structure of the NPs was analyzed by transmission electron microscopy and the morphologies of the NPs and the nanostructured films were studied using high-resolution transmission electron microscopy and scanning electron microscopy. These analyses showed that the original composition and structure of the MPs were preserved in the NPs and films. The majority of NPs that were produced ranged in size between 2 and 15 nm with some larger particles present. Magnetization-temperature curves showed that the films consisted of a mixture of small superparamagnetic NPs and larger ferromagnetic NPs. A high saturation magnetization of 62.3 emu/g at 300 K was retained in the films indicating that they remained free of significant oxidation.     

硫酸软骨素-阳离子表面活性剂缔合物微粒体系的共振散射光谱研究及分析应用

蒋治良,邹明静,梁爱惠,沈星灿. 硫酸软骨素-阳离子表面活性剂缔合物微粒体系的共振散射光谱研究及分析应用[J]. 化学学报, 2006, 64(2): 111-116.     

Al_2O_3/C复合微粒子散射强度分布的计算

利用A .L .Aden和M .Kerker复合微粒子Mie散射理论计算了Al2 O3/C复合微粒子的散射强度分布函数 ,分析了影响散射强度分布的因素     

方腔内微细颗粒物运动特性的格子Boltzmann方法模拟

对Masselot and Chopard提出的模拟气固两相流动的格子Boltzmann-格子气(LBE-LGA)方法进行推广,能够反映两相间拖曳作用.利用该方法研究封闭方腔内的气固两相流运动特性,分析斯托克斯数St和模拟颗粒数目对颗粒群运动的影响,并与文献结果进行比较,表明LBE-LGA方法模拟颗粒运动是可行的.     

DNA assay based on monolayer-barcoded nanoparticles for mass spectrometry in combination with magnetic microprobes

Mass spectrometry (MS) based methodology offers simple, fast and sensitive diagnosis. While it has become the predominate approach in biomolecular analysis, it has not been suitable for analyzing nucleic acid due to its low ionization efficiency. We report herein on a DNA assay based on monolayer-barcoded nanoparticles that were encoded with reporter mass molecules, which act as surrogate molecules for the matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) identification of target DNA through mass spectrometry in combination with magnetic microprobes. This assay demonstrated high MS sensitivity, with the ability to detect target DNA at femtomolar (10⁻¹⁵ M) levels. This inaugural effort using combined techniques is significant because it showed an extraordinary analytical capability for differentiating the single nucleotide polymorphism (SNP), which comprises the most abundant source of genetic variation in the human genome. We also report herein the feasibility of MS detection of two target DNAs that have the same mass but different nucleotide base composition, which classic MS methodology is inherently unable to differentiate.     

Development of biocompatible electrostatic-repulsive microparticles for local tumor treatment

In this study, we reported pH-responsive microparticles consisting of poly(D,L-lactide-co-glycolide) (PLGA), aminated hyaluronic acid (aHA) conjugated with 2,3-dimethylmaleic anhydride (DMA, as a pH-responsive cleavable molecule) (aHA-DMA), and doxorubicin (DOX, as an antitumor drug) for local tumor treatment. The DOX-loaded microparticles, denoted as PLGA(aHA-DMA)/DOX MPs, were fabricated using the W₁/O/W₂ multi-emulsification method. These PLGA(aHA-DMA)/DOX MPs (~10 μm in diameter) accelerated the rate of DOX release at pH 6.8 due to the acidic pH-responsive cleavage of the DMA moieties followed by electrostatic-repulsion between aHA and DOX. This event caused the structural destabilization and collapse of the MPs, leading to the rapid release of DOX. Consequently, the PLGA(aHA-DMA)/DOX MPs resulted in significant inhibition of tumor growth, demonstrating their ability for acidic tumor-specific treatment.     

聚苯乙烯微球表面的聚集态结构和苯基堆砌

用扫描隧道显微镜(STM)研究微乳液聚合法制备的聚苯乙烯微球.STM图像显示这种聚苯乙烯微球的表面具有不同的聚集态结构.在表面的某些区域,聚合物链没有相互缠结现象,而是有序地堆积在一起.在适当的情况下,同一条链上的苯基还会堆砌起来,形成一种平行移位的结构,其类似于苯双分子的最稳定结构.还用STM研究了用苯溶胀过的聚苯乙烯粒子,并观察到了类似螺旋结构的聚合物链以及扭曲的聚合物链.在聚苯乙烯微球中,聚合物链段和苯基的有序堆砌状态的存在,是因为苯乙烯单体已经在微乳液内的某些区域堆砌成有序的状态.