毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸

研究了影响毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸的实验条件.cAMP和cGMP的线性范围分别为30～500mg/L和15～500mg/L,其最低检测限分别为9.0mg/L和0.5mg/L;当浓度为125mg/L时,cAMP和cGMP峰面积的RSD为4.45%和6.21%(n=4);其迁移时间的日间RSD分别为2.92%和2.04%(n=5)     

890 mW Diode Pumped Nd:YVO4 Intracavity-doubled Red Light Laser

A diode-pumped Nd3+:YVO4 emitting at 1342 nm and 671 nm was developed. Low concentration neodymium doped Nd:YVO4 shows excellent stability at 1342 nm. With a type-Ⅱ noncritical phase-matched LBO crystal as the intracavity frequency doubler, 890 mW of 671 nm light was obtained at 11.2 W incident pumping power, the optical-optical conversion efficiency is 7.9%.     

测量中的最佳值及标准误差的数学研究

本文对间接测量量的最可信赖值及误差进行推导 ,方法简明 ,计算公式简单 ,学生易接受。     

Optical pumping and coherence effects in fluorescence from a four level system

An effective four-level system around the D2 line of ⁸⁵Rb at room temperature, is experimentally investigated by fluorescent studies under the action of two driving fields L1 and L2. This system exhibits unique features in fluorescence as a function of frequency separation between L1 and L2. In particular, at two-photon resonance, when the Rabi frequency of L1 exceeds that of L2, signatures of Electromagnetically Induced Transperancy effect (EIT) arising from the three-level Λ sub-system is present as a sub-natural dip in fluorescence from the fourth level. At comparable strengths of L1 and L2 the fluorescence features indicate a regime, where the effects arising from optical pumping and EIT effect due to ground hyperfine level coherence coexist. We see in the coexistence regime, saturation effects arising from difference frequency crossing (DFC) resonances and optical pumping around the EIT window. At low strengths of L1, all signs of coherence vanishes from the system and the fluorescent features result from incoherent optical pumping through the Autler-Townes split states of the excited state hyperfine levels, which are split due to the stronger L2 laser. The dominant role of the L1 laser in creating a robust transparency signal even in the presence of an off-resonant excitation is brought out. The results are supported by density matrix calculations.     

Cross-relaxation and spectral broadening of gain for Nd/Cr:YAG ceramic lasers with white-light pump source under high-temperature operation

Spectral broadening of the fluorescence of a Nd/Cr:YAG ceramic at the 1064 nm lasing wavelength was observed, and the amplification properties at a high temperature were investigated by considering cross-relaxation. These ceramics are promising for use as a solid-laser material pumped with solar or lamp light. It has been found that whenever the temperature of a laser medium is high and a spectral shift occurs, a high small-signal gain remains owing to the broad spectral band and the cross-relaxation. This optical property is remarkably different from that of a Nd:YAG laser. For a conventional Nd:YAG laser, the bandwidth at 1064 nm is 0.45 nm, and a reduction in small-signal gain occurs at a temperature of 373 K because the spectral peak shift is 0.005 nm/K. However, for the Nd/Cr:YAG ceramic, the bandwidths are 1.2 and 1.9 nm in the case of 0.1% Cr ion doping and 3% Cr ion doping, respectively, owing to the existence of excited Cr ions and the shortening of the effective Nd ion coherence time. It is prospected that the laser medium can be used at a high temperature of 600 K.     

Quasi-geostrophic motions in a rotating layer of an electrically conducting fluid

Large-scale nonlinear oscillations of an electrically conducting ideal fluid of varying depth are considered with the magnetic, Archimedean, and Coriolis forces taken into account. The main equations are derived from an analysis of the scales of quasi-geostrophic motions. Under the assumptions that the Rossby numbers (a measure of the ratio of the local and advective accelerations to the Coriolis acceleration) are of the same order, the problem is reduced to a system of three nonlinear equations for hydromagnetic pressure and two functions describing the magnetic field. For an infinitely long horizontal layer of an electrically conducting rotating fluid, the exact solution of the corresponding nonlinear equations and the dispersion relation are obtained under the assumption of an approximately constant slope of the upper boundary surface of the layer at a distance of the order of the wavelength. __________ Translated from Prikladnaya Mekhanika i Tekhnicheskaya Fizika, Vol. 50, No. 1, pp. 30–41, January–February, 2009.     

基于ANN的定向井有杆抽油系统悬点示功图特征参数的计算

有杆抽油系统悬点示功图的特征参数是合理选择地面机电设备的主要依据.由于井下工况的复杂性和部分参数难以确定,使得基于求解高维时变非线性方程的传统方法的计算结果存在偏差.本文将传统方法和神经网络相结合,给出了一种能比较精确地确定定向井有杆抽油系统悬点示功图特征参数的方法,避免了建立和求解复杂的非线性动力学方程.首先依据传统方法,计算出简化悬点示功图的特征参数.然后考虑抽油杆柱弹性振动、抽油杆与油管之问的库仑和粘性摩擦、气体和供液能力等因素的影响,利用BP神经网络和RBF神经网络建立了不同工况下悬点示功图特征参数的计算模型.利用现场实测数据对建立的神经网络进行了训练和测试.测试结果表明了本文方法的正确性和有效性.     

Stability of Poiseuille flow in the presence of a longitudinal magnetic field

The stability of the plane flow of an electrically conducting fluid with respect to small perturbations was studied at large Reynolds numbers in the presence of a longitudinal magnetic field. The dependence of the critical Reynolds number on the electrical conductivity is investigated. At large Reynolds numbers, a new branch of instability and a sudden change in the critical Reynolds numbers is found. __________ Translated from Prikladnaya Mekhanika i Tekhnicheskaya Fizika, Vol. 49, No. 3, pp. 45–53, May–June, 2008.     

Energy Transfers in a System of Two Coupled Oscillators with Essential Nonlinearity: 1:1 Resonance Manifold and Transient Bridging Orbits

The purpose of this study is to highlight and explain the vigorous energy transfers that may take place in a linear oscillator weakly coupled to an essentially nonlinear attachment, termed a nonlinear energy sink. Although these energy exchanges are encountered during the transient dynamics of the damped system, it is shown that the dynamics can be interpreted mainly in terms of the periodic orbits of the underlying Hamiltonian system. To this end, a frequency-energy plot gathering the periodic orbits of the system is constructed which demonstrates that, thanks to a 1:1 resonance capture, energy can be irreversibly and almost completely transferred from the linear oscillator to the nonlinear attachment. Furthermore, it is observed that this nonlinear energy pumping is triggered by the excitation of transient bridging orbits compatible with the nonlinear attachment being initially at rest, a common feature in most practical applications. A parametric study of the energy exchanges is also performed to understand the influence of the parameters of the nonlinear energy sink. Finally, the results of experimental measurements supporting the theoretical developments are discussed. This study was carried out while the author was a postdoctoral fellow at the National Technical University of Athens and at the University of Illinois at Urbana-Champaign.     

Experimental study of non-linear energy pumping occurring at a single fast frequency

Experimental verification of passive non-linear energy pumping in a two-degree-of-freedom system comprising a damped linear oscillator coupled to an essentially non-linear attachment is carried out. In the experiments presented the non-linear attachment interacts with a single linear mode and, hence, energy pumping occurs at a single ‘fast’ frequency in the neighborhood of the eigenfrequency of the linear mode. Good agreement between simulated and experimental results was observed, in spite of the strongly (essentially) non-linear and transient nature of the dynamics of the system considered. The experiments bear out earlier predictions that a significant fraction of the energy introduced directly to a linear structure by an external impulsive (broadband) load can be transferred (pumped) to an essentially non-linear attachment, and dissipated there locally without spreading back to the system. In addition, the reported experimental results confirm that (a) non-linear energy pumping in systems of coupled oscillators can occur only above a certain threshold of the input energy, and (b) there is an optimal value of the energy input at which a maximum portion of the energy is absorbed and dissipated at the NES.