荧光光谱法研究蛋白质构象的电磁-温度协同效应

长期以来电磁生物效应受到人们普遍关注，近年来从各个角度进行了相关研究。电磁－温度协同效应是当前的热点。本研究通过电磁场及温度协同对蛋白质影响的研究，发现蛋白质在电磁场作用下的不可逆变性，而且这种变性也遵循Arrhenius规律，并进一步得出电磁－温度协同作用的蛋白质变性模型。本文从分子反应动力学的角度解释了电磁－温度协同效应，并对非热效应作了一定的探讨。     

Tunneling Radiation of a Torus-Like Black Hole in Anti-de Sitter Space-Time

An extension of the Parikh-Wilczek's semi-classical quantum tunneling method, the tunneling radiation of the charged particle from a torus-like black hole is investigated. Difference from the uncharged mass-less particle, the geodesics of the charged massive particle tunneling from the black hole is not light-like, but determined by the phase velocity. The derived result shows that the tunneling rate depends on the emitted particle's energy and electric charge, and takes the same functional form as uncharged particle. It proves also that the exact emission spectrum is not strictly pure thermal, but is consistent with the underlying unitary theory. PACS Numbers: 04.70.Dy, 97.60.Lf, 05.30.Ch.     

Surface free energy of CrNx films deposited using closed field unbalanced magnetron sputtering

CrN_x thin films have attracted much attention for semiconductor IC packaging molding dies and forming tools due to their excellent hardness, thermal stability and non-sticking properties (low surface free energy). However, few data has been published on the surface free energy (SFE) of CrN_x films at temperatures in the range 20-170 °C. In this study CrN_x thin films with CrN, Cr(N), Cr₂N (and mixture of these phases) were prepared using closed field unbalanced magnetron sputtering at a wide range of Cr⁺² emission intensity. The contact angles of water, di-iodomethane and ethylene glycol on the coated surfaces were measured at temperatures in the range 20-170 °C using a Dataphysics OCA-20 contact angle analyzer. The surface free energy of the CrN_x films and their components (e.g., dispersion, polar) were calculated using the Owens-Wendt geometric mean approach. The influences of CrN_x film surface roughness and microstructure on the surface free energy were investigated by atomic force microscopy (AFM) and X-ray diffraction (XRD), respectively. The experimental results showed that the lowest total SFE was obtained corresponding to CrN at temperature in 20 °C. This is lower than that of Cr(N), Cr₂N (and mixture of these phases). The total SFE, dispersive SFE and polar SFE of CrN_x films decreased with increasing surface temperature. The film roughness has an obvious effect on the SFE and there is tendency for the SFE to increase with increasing film surface roughness.     

Critical points in the Bragg glass phase of a weakly pinned crystal of Ca3Rh4Sn13

New experimental data are presented on the scan rate dependence of the magnetization hysteresis width ΔM(H) (∞ critical current densityJ _c(H)) in isothermalMH scans in a weakly pinned single crystal of Ca₃Rh₄Sn₁₃, which displays second magnetization peak (SMP) anomaly as distinct from the peak effect (PE). We observe an interesting modulation in the field dependence of a parameter which purports to measure the dynamical annealing of the disordered bundles of vortices injected through the sample edges towards the destined equilibrium vortex state at a givenH. These data, in conjunction with the earlier observations made while studying the thermomagnetic history dependence inJ _c(H) in the tracing of the minor hysteresis loops, imply that the partially disordered state heals towards the more ordered state between the peak field of the SMP anomaly and the onset field of the PE. The vortex phase diagram in the given crystal of Ca₃Rh₄Sn₁₃ has been updated in the context of the notion of the phase coexistence of the ordered and disordered regions between the onset field of the SMP anomaly and the spinodal line located just prior to the irreversibility line. A multi-critical point and a critical point in the (H,T) region of the Bragg glass phase have been marked in this phase diagram and the observed behavior is discussed in the light of recent data on multi-critical point in the vortex phase diagram in a single crystal of Nb.     

Dibutyltin-3-hydroxyflavone titrates a dissociable component (cofactor) of mitochondrial ATP synthase: An energy-transfer component linked to the ubiquinone pool

Dibutyltin-3-hydroxyflavone, Bu₂Sn(of), is a new fluorescence probe inhibitor of F₁F₀-ATPase and oxidative phosphorylation which inhibits by titration of an unidentified component of F₀. Its site of action is closely related to that of the trialkyltins and of venturicidin. This F₀ component is part of a pool of this component which is present in the heart mitochondrial inner membrane at levels of 5–7 nmol (mg protein)^?1 [18 ± 3 Bu₂Sn(of) sites per mol F₁F₀-ATPase]. However, ATPase activity in submitochondrial particles is near maximally inhibited by titration of approx. three Bu₂Sn(of) sites per mol F₁F₀-ATPase. Over 60% (60–80%) of the Bu₂Sn(of) interaction sites can be lost during the purification of F₁F₀-ATPase from submitochondrial particles. The number of Bu₂Sn(of) interaction sites in various F₁F₀-ATPase preparations is variable. The high numbers of Bu₂Sn(of) sites per mol F₁F₀-ATPase for heart mitochondria (18–21) and submitochondrial particles (15–19.5) decline in ATP synthase (11–15) to the low values obtained in Complex V (7–10.5) and the minimal values observed in highly purified F₁F₀?ATPase (3.5–5.6), thus indicating a variable dissociable component or cofactor of ATP synthase. The Bu₂Sn(of) interaction site, a component of ATP synthase, is responsive to the redox status of the respiratory chain and the interaction with Bu₂Sn(of) is with the reduced form of this component. Fluorescence titration studies show that this component is in redox equilibrium with the ubiquinone pool of the respiratory chain. It is proposed that this redox component serves as an inhibitor titratable cofactor pool which cycles through an F₀ interaction site (or sites) via a system which serves as an energy-transfer link between the respiratory chain and ATP synthase.     

Sorption characteristics of polyfluorocarboxamides

The dependence of the variations in the differential molar free energy of sorption of polyfluorocarboxamides R_FC(O)NR₂ (R_F = CF₃(CF₂) _i ,i=0 to 5, R=H or Me) on capillary columns on the molecular structure of the amides, the temperature of analysis, and the nature of the stationary phases (OV-101, XE-60, and PEG-40M) were investigated by GLC. The peculiarities of the variation of the sorption characteristics of amides in XE-60 and PEG-40M polar phases, which cause the inversion of the order of retention of the members of pseudohomologous series, were found.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 868–871, May, 1994.We are grateful to R. V. Golovnya and D. N. Grigor'eva (Institute of Food Substances of the RAS, Moscow) for their participation in the discussion of the results obtained.     

The decisive role of calorimetric measurements in equilibrium phase diagram calculations

In this paper, examples are given of how calorimetric values can give greater certainty to phase equilibria calculated from thermodynamic data. Errors that may arise when phase diagram evaluations are carried out largely from the basis of Gibbs energy information only are illustrated by reference to recent evaluations of the Ti?Si system and the resulting calculated oxidation behaviour of titanium silicides. The importance of calorimetric values for calculation of metastable phase equilibria is demonstrated by results of work on the AlN?TiN hard-metal coating system. Finally, suggestions are made with regard to areas of work where calorimetric data are urgently needed.     

教学研究:介绍量子力学几个基本概念——兼答《关于量子几何相位的评注》中的几个主要问题

介绍了量子绝热定理的物理含义及成立的条件，认为有关主要献（Aharonov-Anandan,Bohm，孙昌璞等）的表述是正确的，而《关于量子几何相位的评注》^[1]（以下简称《评注》）相应的表述不完全正确。在此基础上，认为这些献和教材（R.Shankar)得出的涉及Berry绝热相位的一些论述（不含Berry绝热相因子的瞬时能量本征态不满足含时Schroedinger方程等）也是正确的，而《评注》的论述与此相反。《评注》认为只有γn(C)才是Berry相位。本作则倾向于把γn（t）叫做Berry绝热相位，而把γn（C）=γn（T）-γn（0）叫做几何相位（geometric phase)^[2]。     

Energy inconsistency of Galerkin approximationsfor plane Couette flow

For classical solutions of the incompressible Navier-Stokes equations (NSE) the energybalance between kinetic energy, work done by external forces, and viscous dissipation holds rigorously true. It is shown in this paper that standard Galerkin approximations violate energy balance in the case of plane Couette flow, whereas Poiseuille flow turns out to be energy consistent at any cutoff. The main reason for this discrepancy is seen in the different boundary conditions between the stationary linear shear flow and its disturbances. In our analysis, essentially, we introduce an auxiliary external force field which enforces the finite dimensional Galerkin approximation to fulfil the NSE. It is exemplarily demonstrated how the energy discrepancy decreases when the number of disturbed modes is increased which couple to the stationary shear flow.     

Ultra-high energy cosmic rays and prompt TeV gamma rays from gamma ray bursts

Gamma ray bursts (GRBs) have been proposed as one possible class of sources of the ultrahigh energy cosmic ray (UHECR) events observed up to energies ≳ 10²⁰ eV. The synchrotron radiation of the highest energy protons accelerated within the GRB source should produce gamma rays up to TeV energies. Here we briefly discuss the implications on the energetics of the GRB from the point of view of the detectability of the prompt TeV γ-rays of proton-synchrotron origin in GRBs in the up-coming ICECUBE muon detector in the south pole.