Establishment and Application of a Predictive Growth Kinetic Model of Salmonella with the Appearance of Two Other Dominant Background Bacteria in Fresh Pork

To better guide microbial risk management and control, growth kinetic models of Salmonella with the coexistence of two other dominant background bacteria in pork were constructed. Sterilized pork cutlets were inoculated with a cocktail of Salmonella Derby (S. Derby), Pseudomonas aeruginosa (P. aeruginosa), and Escherichia coli (E. coli), and incubated at various temperatures (4–37 °C). The predictive growth models were developed based on the observed growth data. By comparing R² of primary models, Baranyi models were preferred to fit the growth curves of S. Derby and P. aeruginosa, while the Huang model was preferred for E. coli (all R² ≥ 0.997). The secondary Ratkowsky square root model can well describe the relationship between temperature and μ_max (all R² ≥ 0.97) or Lag (all R² ≥ 0.98). Growth models were validated by the actual test values, with B_f and A_f close to 1, and MSE around 0.001. The time for S. Derby to reach a pathogenic dose (10⁵ CFU/g) at each temperature in pork was predicted accordingly and found to be earlier than the time when the pork began to be judged nearly fresh according to the sensory indicators. Therefore, the predictive microbiology model can be applied to more accurately predict the shelf life of pork to secure its quality and safety.     

残余寿命熵的上界

讨论寿命分布混合的类不变性问题,改进了文[1]中的相关结果.此外,基于寿命分布类的特性研究了不同于[4]的另一类残余寿命熵的特性,并在一定的条件下得到残余寿命熵的上界.     

事件是如何生成的？—道光的日常与“鸦片战争”作为事件的形成

借助相对更接近历史现场的《起居注》,从道光十二年闰九月观察道光十八年到二十二年,看这几年的经历如何生成为“鸦片战争”的“历史叙述”。拉长镜头,尽可能贴近其中一个现场,顺时而观,从经历,到散落在时序中的记录,到秘密编纂的分类事务（夷务）,到事件,再到划时代的事件。对照清朝自身的“事件”生产机制,可以看清在不同目的的驱使下,时人的经历与处置、事后诸人的思考、外人的叙述如何交汇,时人如何“失语”,后人如何控制了历史叙述,“事件”如何从经历中脱颖而出、意义又是如何被不断增添、改变与筛减的,“事件”形成后又如何变为重返历史现场的一层障碍。了解这些,我们便有可能从更多的角度去重新发现这段历史,并对“事件史”的意义与局限有更真切的认识。     

Comparative Phosphoproteomic Analysis of Sporulated Oocysts and Tachyzoites of Toxoplasma gondii Reveals Stage-Specific Patterns

Toxoplasma gondii is an obligate intracellular protozoan of severe threat to humans and livestock, whose life history harbors both gamic and apogamic stages. Chinese 1 (ToxoDB#9) was a preponderant genotype epidemic in food-derived animals and humans in China, with a different pathogenesis from the strains from the other nations of the world. Posttranslational modifications (PTMs) of proteins were critical mediators of the biology, developmental transforms, and pathogenesis of protozoan parasites. The phosphoprotein profiling and the difference between the developmental phases of T. gondii, contributing to development and infectivity, remain unknown. A quantitative phosphoproteomic approach using IBT integrated with TiO₂ affinity chromatography was applied to identify and analyze the difference in the phosphoproteomes between the sporulated oocysts and the tachyzoites of the virulent ToxoDB#9 (PYS) strain of T. gondii. A total of 4058 differential phosphopeptides, consisting of 2597 upregulated and 1461 downregulated phosphopeptides, were characterized between sporulated the oocysts and tachyzoites. Twenty-one motifs extracted from the upregulated phosphopeptides contained 19 serine motifs and 2 threonine motifs (GxxTP and TP), whereas 16 motifs identified from downregulated phosphopeptides included 13 serine motifs and 3 threonine motifs (KxxT, RxxT, and TP). Beyond the traditional kinases, some infrequent classes of kinases, including Ab1, EGFR, INSR, Jak, Src and Syk, were found to be corresponding to motifs from the upregulated and downregulated phosphopeptides. Remarkable functional properties of the differentially expressed phosphoproteins were discovered by GO analysis, KEGG pathway analysis, and STRING analysis. S8GFS8 (DNMT1-RFD domain-containing protein) and S8F5G5 (Histone kinase SNF1) were the two most connected peptides in the kinase-associated network. Out of these, phosphorylated modifications in histone kinase SNF1 have functioned in mitosis and interphase of T. gondii, as well as in the regulation of gene expression relevant to differentiation. Our study discovered a remarkable difference in the abundance of phosphopeptides between the sporulated oocysts and tachyzoites of the virulent ToxoDB#9 (PYS) strain of T. gondii, which may provide a new resource for understanding stage-specific differences in PTMs and may enhance the illustration of the regulatory mechanisms contributing to the development and infectivity of T. gondii.     

基于PHM的舰艇装备视情维修技术研究

针对PHM技术在舰艇装备视情维修中的应用需求,在分析剩余寿命预测机理的基础上,为解决舰艇装备历史数据少、状态变化普遍表现为非线性等难题,建立基于粒子滤波的多步剩余寿命预测模型,通过仿真表明：随着时间的推移剩余寿命PDF图变狭窄,剩余寿命预测误差逐渐降低,证明了预测方法的有效性。在此基础上,考虑装备维修费用最低为目标,建立以费用最低为目标的预防性维修时间确定模型,使得单位时间内的平均维修费用最低,并通过采用建立的基于粒子滤波的多步剩余寿命预测模型,最终确定了最优预防性维修时间,为装备保障人员拟订精确维修保障计划提供依据,也为舰艇装备开展视情维修奠定基础。     

Effect of collimator size and absorber thickness on gamma ray attenuation measurements for bakelite and perspex

The mass attenuation coefficient (μ _m) of 662 keV gamma rays have been measured in the extended media of bakelite and perspex under different collimation conditions. The increase in attenuation coefficient is seen with increase in sample thickness as well as with collimator size due to the contribution of multiple scattered photons in the uncollided beam of 662 keV gamma rays.     

Synthesis of molecular motors incorporating para-phenylene-conjugated or bicyclo[2.2.2]octane-insulated electroactive groups

The insulating role of the bicyclo[2.2.2]octane fragment has been theoretically evaluated by comparing the electronic coupling parameter (V(ab)) in 1,4-bis(ferrocenyl)benzene (1) and 1,4-bis(ferrocenyl)bicyclo[2.2.2]octane (2). The geometries were optimized by DFT and an extended Hückel calculation was performed to evaluate V(ab) by the dimer splitting method. The calculations showed a 12-fold decrease of the electronic coupling from 60 meV for 1 to 5 meV for 2. The second part describes the synthesis of two potential molecular motors with one incorporating the insulating bicyclo[2.2.2]octane fragment. These molecules are based on a ruthenium complex bearing a tripodal stator functionalized to be anchored onto surfaces. The ferrocenyl electroactive groups and the cyclopentadienyl (Cp) rotor are connected through a p-phenylene spacer (5) or through a spacer incorporating an insulating bicyclo[2.2.2]octane moiety (6).     

Nondestructive evaluation for remanent life of modified 9Cr-1Mo steel by reversible magnetic permeability

We present a magnetic and nondestructive method to evaluate the remanent life of modified 9Cr-1Mo steel by measuring the reversible magnetic permeability. Specimens with ten different kinds of aging periods were prepared using an isothermal heat treatment at 690 °C. The Larson-Miller parameter (LMP) was calculated and the peak interval of reversible magnetic permeability (PIRMP) was measured using the surface type probe. PIRMP was inversely proportional to LMP. We can evaluate the remanent life of modified 9Cr-1Mo steel using the relationship between PIRMP and LMP. Also, we present the possibility that the tensile strength and yield strength measured by destructive methods could be estimated by PIRMP measured nondestructively.     

TiO2基光解水析氢非贵金属共催化剂的研究

利用太阳能光催化水解制氢是获得清洁、廉价、无污染的氢气最有前景的一种方式。这个过程主要包括三个步骤：太阳光的捕获,电荷分离与转移,催化质子还原产生氢气。其中,大量的研究工作主要集中在前两步,对于第三步的研究则相对较少。然而,共催化剂的引入可以有效促进光催化活性并提高氢气产生速率。共催化剂主要分为贵金属共催化剂和非贵金属共催化剂,其中,贵金属共催化剂有着较高的活性,但是其价格及来源限制了其实际应用,因此开发廉价高效的非贵金属共催化剂非常重要。本文对TiO₂基光解水析氢的非贵金属共催化剂（过渡金属单质及其复合物以及非金属碳基材料）进行了总结,详细讨论了不同共催化剂的作用机理,并对共催化剂的发展方向进行了合理展望。