Fluorimetric Determination of Histamine in Fish Using Micellar Media and Fluorescamine as Labelling Reagent

An analytical method based on the use of fluorescamine to produce a fluorescent derivative with histamine and combined with micellar-enhanced fluorescence detection of the formed complex is developed for the sensitive and rapid determination of histamine in fishes. The fluorescence properties of the obtained complex in water and micellar solutions of sodium dodecyl sulfate (SDS), cetyltrimethylammonium chloride (CTAC) and brij-700 are reported. Physicochemical variables influencing the sensitivity of the method (pH, micellar, fluorescamine and NaCl relative concentrations) have been optimized. The stability of the formed complex, as shown by kinetic study, depends on the pH of the solution. Linear calibration curves allowing an effective histamine determination were established with large linear dynamic range (LDR), and low limits of detection (LOD) between 0.5 and 33 ng mL⁻¹, according to the solvent. Application to the analysis of fish samples (sardines) yielded satisfactory results. The method seems to be suitable for environmental fish quality control. Presented in part, at the 39th IUPAC Congress and the 86th Conference of the Canadian Society for Chemistry, August, 10–15, 2003, Ottawa (Canada).     

An ODE Model of the Motion of Pelagic Fish

A system of ordinary differential equations (ODEs) is derived from a discrete system of Vicsek, Czirók et al. [Phys. Rev. Lett. 75(6):1226–1229, 1995], describing the motion of a school of fish. Classes of linear and stationary solutions of the ODEs are found and their stability explored using equivariant bifurcation theory. The existence of periodic and toroidal solutions is also proven under deterministic perturbations and structurally stable heteroclinic connections are found. Applications of the model to the migration of the capelin, a pelagic fish that undertakes an extensive migration in the North Atlantic, are discussed and simulation of the ODEs presented.     

Food Web Implications of δ13C and δ15N Variability over 370 km of the Regulated Colorado River USA

Abstract

Dual stable isotope analysis in the regulated Colorado River through Grand Canyon National Park, USA, revealed a food web that varied spatially through this arid biome. Down-river enrichment of δ¹³C data was detected across three trophic levels resulting in shifted food webs. Humpack chub δ¹³C and δ¹⁵N values from muscle plugs and fin clips did not differ significantly. Humpback chub and rainbow trout trophic position is positively correlated with standard length indicating an increase in piscivory by larger fishes. Recovery of the aquatic community from impoundment by Glen Canyon Dam and collecting refinements for stable isotope analysis within large rivers are discussed.     

Misalignment Fault Prediction of Wind Turbines Based on Improved Artificial Fish Swarm Algorithm

A misalignment fault is a kind of potential fault in double-fed wind turbines. The reasonable and effective fault prediction models are used to predict its development trend before serious faults occur, which can take measures to repair in advance and reduce human and material losses. In this paper, the Least Squares Support Vector Machine optimized by the Improved Artificial Fish Swarm Algorithm is used to predict the misalignment index of the experiment platform. The mixed features of time domain, frequency domain, and time-frequency domain indexes of vibration or stator current signals are the inputs of the Least Squares Support Vector Machine. The kurtosis of the same signals is the output of the model, and the 3σ principle of the normal distribution is adopted to set the warning line of misalignment fault. Compared with other optimization algorithms, the experimental results show that the proposed prediction model can predict the development trend of the misalignment index with the least prediction error.     

The analyses of alkyllead compounds in fish and environmental samples in Ontario,Canada (1981–1987)

Analyses of fish and other environmental samples (clams, macrophytes, sediments and waters) from areas upstream and downstream from two alkyllead manufactures beside the St Lawrence and St Clair Rivers, Ontario, show a clear indication of elevated alkyllead levels in samples near the industries. Most species of fish contained alkyllead compounds with tetraethyllead and triethyllead as the predominant forms. Most fish from the contaminated areas contained 50–75% of total lead as alkylleads. Carp, yellow perch and white sucker were generally the most contaminated species while pike, alewife and rock bass were the least contaminated. Average alkyllead levels varied from year to year but declined steadily after 1981. For example, the geometric mean of alkyllead compounds in carp from the St Lawrence River decreased from 4207 μg kg^?1 in 1981 to 2000 μg kg^?1 in 1982 and to 49 μg kg^?1 in 1987, reflecting the reduction of alkylleads in the effluents and the closure of one of the manufactures in 1985. Alkyllead levels were consistently lower in muscle and carcass samples in comparison with whole fish containing fatty intestines. However, muscle levels were generally equal to carcass levels. The concentrations of alkyllead compounds were generally low in clams, macrophytes, sediments and waters except from the immediate vicinity of the manufactures' final effluent discharges.     

加速溶剂萃取-高效液相色谱-串联质谱法同时检测鱼肉中喹诺酮、磺胺与大环内酯类抗生素

建立了加速溶剂萃取(ASE)-高效液相色谱-串联质谱联用法同时检测鱼肉中22种抗生素药物残留的分析方法。样品经ASE提取,HLB固相萃取柱净化后进入高效液相色谱-串联质谱仪分析。对ASE的萃取条件进行优化,并采用XTerraMSC18柱对药物进行分离,以甲醇-乙腈(体积比1∶1)为色谱流动相A,以0.3%(体积分数)甲酸水溶液(含0.1%甲酸铵,pH=2.9)为流动相B。22种喹诺酮、磺胺和大环内酯类抗生素药物在加标水平为20、100μg/L时的回收率分别为72%～120%与66%～114%,相对标准偏差(RSD,n=5)分别为1.9%～16%与0.7%～10%,方法的检出限为0.02～0.6μg/kg。结果显示所建立的方法精密度好,准确度高,可满足同时对鱼肉样品中多种喹诺酮、磺胺和大环内酯类抗生素残留进行定性及定量分析的要求。     

DNA damage induced by organotins on ­trout‐nucleated erythrocytes

The effect of tributyltin‐chloride (TBTC), dibutyltin‐chloride (DBTC) and monobutyltin‐chloride (MBTC) on rainbow trout (Salmo irideus) nuclear DNA, was investigated by means of single cell gel electrophoresis (‘comet’ assay). Our data show that TBTC presents a marked genotoxic effect, whereas the genotoxic effect is less pronounced for DBTC and it is completely absent for MBTC. These results could be important in evaluating the environmental risks deriving from the use of these molecules as a antifouling agents in marine paints and as agricultural biocides. Copyright © 2001 John Wiley & Sons, Ltd.

Abbreviations:

TBTC,

tributyltin‐chloride;

DBTC,

dibutyltin‐chloride;

MBTC,

monobutyltin‐chloride;

Hb,

hemoglobin.

     

Teil II: Ergebnisse bei der Bestrahlung einzelner Nahrungsmittelgruppen (Zusammenfassender Bericht)

Nachdem im ersten Teil dieser Arbeit [170] einige Grundprobleme der Nahrungsmittelbestrahlung behandelt wurden, sollen nun die bei der Bestrahlung wichtiger Nahrungsmittelgruppen erzielten Ergebnisse dargelet und eine Einschätzung der Entwicklungstendenzen versucht werden.     

内蒙古段黄河水系放射性水平调查研究

给出了内蒙古段黄河水平中总α，总β，＾９０Ｓｒ，＾１３７Ｃｓ，＾４０Ｋ，＾３Ｈ，＾２１０Ｐｂ，＾２１０Ｐｏ，＾２３８Ｕ，＾２３２Ｔｈ、＾２２６Ｒａ放射性水平，结果基本为正常水平，找出了污染源，为内蒙古段黄河水中放射性监督监测建立了全面的背景材料。     

Ionic alkyl-lead,tetra-alkyl-lead and total lead in fish from the Great Lakes

Fillets from a variety of fish species collected from Lakes Ontario, Superior and Erie, Canada, were examined for ionic alkyl-lead, tetra-alkyl-lead and total lead compounds. Diphenylthiocarbazone (dithizone)-derivatized extracts were collected at pH 8 and 9 for ionic alkyl-leads from enzymatically hydrolyzed samples. Butylated derivatives were formed prior to analysis by gas chromatography-atomic absorption spectrometry (GC AA). Tetra-alkyl-lead was extracted from the hydrolyzates with hexane. Most of the fillets contained low (<0.08–2 ng g^?1) levels of trimethyl-lead. Several samples contained triethyl-lead or tetraethyl-lead. Dimethyl-lead, diethyl-lead and tetramethyl-lead were detected by GC MS but were below the GC AA method detection limit of 0.06 ng g^?1, 0.09 ng g^?1 and 0.2 ng g^?1 respectively. Total lead levels were between <1.8 and 96.7 ng g^?1.