A comprehensive review of the development of assays, bioprobes, and biosensors using quantum dots (QDs) as integrated components is presented. In contrast to a QD that is selectively introduced as a label, an integrated QD is one that is present in a system throughout a bioanalysis, and simultaneously has a role in transduction and as a scaffold for biorecognition. Through a diverse array of coatings and bioconjugation strategies, it is possible to use QDs as a scaffold for biorecognition events. The modulation of QD luminescence provides the opportunity for the transduction of these events via fluorescence resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET), charge transfer quenching, and electrochemiluminescence (ECL). An overview of the basic concepts and principles underlying the use of QDs with each of these transduction methods is provided, along with many examples of their application in biological sensing. The latter include: the detection of small molecules using enzyme-linked methods, or using aptamers as affinity probes; the detection of proteins via immunoassays or aptamers; nucleic acid hybridization assays; and assays for protease or nuclease activity. Strategies for multiplexed detection are highlighted among these examples. Although the majority of developments to date have been in vitro, QD-based methods for ex vivo biological sensing are emerging. Some special attention is given to the development of solid-phase assays, which offer certain advantages over their solution-phase counterparts. 相似文献
Human motion induced vibration has very low frequency, ranging from 2 Hz to 5 Hz. Traditional vibration isolators are not effective in low-frequency regions due to the trade-off between the low natural frequency and the high load capacity. In this paper, inspired by the human spine, we propose a novel bionic human spine inspired quasi-zero stiffness (QZS) vibration isolator which consists of a cascaded multi-stage negative stiffness structure. The force and stiffness characteristics are investigated first, the dynamic model is established by Newton’s second law, and the isolation performance is analyzed by the harmonic balance method (HBM). Numerical results show that the bionic isolator can obtain better low-frequency isolation performance by increasing the number of negative structure stages, and reducing the damping values and external force values can obtain better low-frequency isolation performance. In comparison with the linear structure and existing traditional QZS isolator, the bionic spine isolator has better vibration isolation performance in low-frequency regions. It paves the way for the design of bionic ultra-low-frequency isolators and shows potential in many engineering applications.
Combined with third generation synchrotron radiation light sources, X-ray photoelectron spectroscopy (XPS) with higher energy resolution, brilliance, enhanced surface sensitivity and photoemission cross section in real time found extensive applications in solid-gas interface chemistry. This paper reports the calculation of the core-level binding energy shifts (CLS) using the first-principles density functional theory. The interplay between the CLS calculations and XPS measurements to uncover the structures,... 相似文献
