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91.
An improved version of the disposable multichannel immunochemical biosensor for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) based on a screen-printed amperometric transducer and monoclonal antibodies (MAb) against 2,4-D is reported. Entrapment within a thin Nafion film was used for the direct immobilization of MAb at the electrode surface. The amount of the tracer (2,4-D conjugated to acetylcholinesterase) bound in a competitive immunochemical reaction was determined amperometrically using acetylthiocholine iodide as substrate. The measuring procedure (times of incubation with tracer and substrate, pH, tracer concentration) was optimized. The sensor was able to detect less than 0.01 μg/L of free 2,4-D in water. One analysis (8 samples) was completed in 30 min (20 min for immunochemical reaction, 5 min incubation with substrate, 5 min measurement). The performance of the immunosensor (two configurations) was evaluated on real samples (tap water) with added 2,4-D. The determined amounts (mean values 0.097 to 0.105 and 0.89 to 1.13) corresponded well with the added contents of 2,4-D (0.100 and 1.00 μg/L, respectively). 相似文献
92.
93.
分体插接式光纤光栅应变片设计与实现 总被引:1,自引:0,他引:1
提出一种适合作为二次变换元件使用的通用型应变传感预制结构——光纤光栅应变片.它以裸Bragg光栅为研究对象,采用分体式设计方法把敏感光栅与连接光纤分别封装于独立的基体中,并通过两个基体相互之间的插入实现了Bragg光栅与测量光路的机械连接.理论分析和实验研究表明:光纤光栅应变片具有与Bragg光栅相同的反射谱,其测量线性度好,灵敏度高,温度误差则随被测试件性质不同而变化,当试件材料与基底材料一致时,温度误差可以忽略. 相似文献
94.
Martina Vizza Patrick Marcantelli Claudia Giovani Walter Giurlani Paolo Giusti Claudio Fontanesi Massimo Innocenti 《Molecules (Basel, Switzerland)》2022,27(10)
Recently, the new updates in legislation about drinking water control and human health have increased the demand for novel electrochemical low-cost sensors, such as potentiometric ones. Nowadays, the determination of chloride ion in aqueous solutions has attracted great attention in several fields, from industrial processes to drinking water control. Indeed, chloride plays a crucial role in corrosion, also influencing the final taste of beverages, especially coffee. The main goal is to obtain devices suitable for continuous and real-time analysis. For these reasons, we investigated the possibility to develop an easy, low-cost potentiometric chloride sensor, able to perform analysis in aqueous mediums for long immersion time and reducing the need of periodic calibration. We realized a chloride ion selective electrode made of Ag/AgCl sintered pellet and we tested its response in model solutions compatible with drinking water. The sensor was able to produce a stable, reproducible, and accurate quantification of chloride in 900 s, without the need for a preliminary calibration test. This opens the route to potential applications of this sensor in continuous, in situ, and real time measurement of chloride ions in industrial processes, with a reduced need for periodic maintenance. 相似文献
95.
Leqing Lin Yu Zhong Haoyang Lin Chenglong Wang Zhifei Yang Qian Wu Di Zhang Wenguo Zhu Yongchun Zhong Yuwei Pan Jianhui Yu Huadan Zheng 《Molecules (Basel, Switzerland)》2022,27(13)
Spider silk is one of the hottest biomaterials researched currently, due to its excellent mechanical properties. This work reports a novel humidity sensing platform based on a spider silk-modified quartz tuning fork (SSM-QTF). Since spider silk is a kind of natural moisture-sensitive material, it does not demand additional sensitization. Quartz-enhanced conductance spectroscopy (QECS) was combined with the SSM-QTF to access humidity sensing sensitively. The results indicate that the resonance frequency of the SSM-QTF decreased monotonously with the ambient humidity. The detection sensitivity of the proposed SSM-QTF sensor was 12.7 ppm at 1 min. The SSM-QTF sensor showed good linearity of ~0.99. Using this sensor, we successfully measured the humidity of disposable medical masks for different periods of wearing time. The results showed that even a 20 min wearing time can lead to a >70% humidity in the mask enclosed space. It is suggested that a disposable medical mask should be changed <2 h. 相似文献
96.
Quentin Coquerel Claire Legendre Jacinthe Frangieh Stephan De Waard Jrme Montnach Leos Cmarko Joseph Khoury Charifat Said Hassane Dimitri Brard Benjamin Siegler Ziad Fajloun Harold De Pomyers Kamel Mabrouk Norbert Weiss Daniel Henrion Pascal Richomme Csar Mattei Michel De Waard Anne-Marie Le Ray Christian Legros 《Molecules (Basel, Switzerland)》2022,27(13)
Voltage-gated Na+ (NaV) channels are significant therapeutic targets for the treatment of cardiac and neurological disorders, thus promoting the search for novel NaV channel ligands. With the objective of discovering new blockers of NaV channel ligands, we screened an In-House vegetal alkaloid library using fluorescence cell-based assays. We screened 62 isoquinoline alkaloids (IA) for their ability to decrease the FRET signal of voltage sensor probes (VSP), which were induced by the activation of NaV channels with batrachotoxin (BTX) in GH3b6 cells. This led to the selection of five IA: liriodenine, oxostephanine, thalmiculine, protopine, and bebeerine, inhibiting the BTX-induced VSP signal with micromolar IC50. These five alkaloids were then assayed using the Na+ fluorescent probe ANG-2 and the patch-clamp technique. Only oxostephanine and liriodenine were able to inhibit the BTX-induced ANG-2 signal in HEK293-hNaV1.3 cells. Indeed, liriodenine and oxostephanine decreased the effects of BTX on Na+ currents elicited by the hNaV1.3 channel, suggesting that conformation change induced by BTX binding could induce a bias in fluorescent assays. However, among the five IA selected in the VSP assay, only bebeerine exhibited strong inhibitory effects against Na+ currents elicited by the hNav1.2 and hNav1.6 channels, with IC50 values below 10 µM. So far, bebeerine is the first BBIQ to have been reported to block NaV channels, with promising therapeutical applications. 相似文献
97.
Partial P-type metal ions doping(PPMID) is an alternative method to further enhance the gas sensing performance of N-type metal oxides(NMOs) in contrast to that of P-N metal oxides heterojunctions, but the influences of the introduction of PPMID on the grain size and oxygen vacancies of NMOs have been rarely investigated. Herein, a simple and effective route has been demonstrated to address this problem with Cu2+-doped SnO2 metastable solid solution nanofibers(CSMSSNs) as model and C2H2 as target molecule by combining electrospinning and calcination technique. It seems that the introduction of PPMID can also affect crystal structure and oxygen vacancies of NMOs, proven by combining X-ray diffraction(XRD) and X-ray photoelectron spectra(XPS). Thus, PPD, crystal structure and oxygen vacancies have been combined to clarify the enhanced sensing performance of Cu-doped SnO2 metastable solid solution nanofibers angainst C2H2. 相似文献
98.
利用氧荧光猝灭原理研制了台式生化需氧量(BOD)传感检测仪器。仪器的工作系统由检测系统和软件系统两部分组成。该检测仪操作简便,检测快速,可进行海水BOD含量的现场检测。仪器的性能考察结果表明,在0.5~50mg/L浓度范围内BOD响应的线性相关系数为0.9978。实际海水样品的检测结果显示,仪器对海水样品的平行测定结果相对标准偏差小于±5%,并且与标准五日生化需氧量(BOD5)方法所获得的数值的相关性为0.99。仪器具有较长的使用寿命和良好的稳定性。 相似文献
99.
100.
一种具有声低通滤波特性的无源零差光纤水听器 总被引:2,自引:1,他引:1
报道了一种新颖的具有抗混叠功能的无源零差迈克耳孙型光纤水听器.它由一个普通的芯轴型光纤水听器和一个圆柱型亥姆霍兹共振器构成.在驻波罐中对其声压相位灵敏度频响进行了测量,结果表明该光纤水听器具有较好的声低通滤波特性,能有效地抑制声信号中的高频成分,从而实现抗混叠滤波.该光纤水听器的低频声压相位灵敏度主要由传感光纤长度和弹性增敏层的物理特性决定,约为-159 dB(0 dB=1 rad/μPa).在1150 Hz附近出现了一个共振峰,这主要由圆柱型亥姆霍兹共振器的声学特性决定.1150~2280 Hz频段内的灵敏度衰减率约为50 dB/倍频程,1500 Hz以后的灵敏度衰减量大于10 dB.这对于提高我国未来声纳系统的抗干扰能力具有十分重要的意义. 相似文献