PARADOXICAL EFFECT OF A GnRH AGONIST ON STEROIDOGENESIS IN CULTURED MONKEY GRANULOSA CELLS

In this study we demonstrate: (i) The GnRH agonist exerts a direct dose-dependet stimulative effect on the aromatase activity and progesterone production in cultured monkey granulosa cells; (ii)the stimulative effect on steroidogenesis can be completely blocked by concomitant treatment with a GnRH antagonist, suggesting that the actions of GnRH are mediated through stringent stereospecific recongnition sites; (iii) in addition to the stimulative effect, the GnRH agonist in the presence of gonadotropins also exerts an inhibitory effect, even though the peptide by itself is more effective in the stimulation of steroidogenesis, and the stimulation of gonadotropin on steroidogenesis could be gradually restored by decreasing the concentration of the GnRH agonist in the culture; and (iv) paradoxical effect can also be observed in the presence of cAMP-inducing agents, suggesting that the inhibitory action of the peptide on gonadotropin-induced steroidogenesis is localized at a step distal to the stringent reco     

Transesterification of an RNA model in buffer solutions in H2O and D2O

Transesterification of a phosphodiester bond of RNA models has been studied in various buffer solutions, under neutral and slightly alkaline conditions in H₂O and D₂O. The results show that imidazole is the only buffer system where a clear buffer catalysis on the cleavage of a phosphodiester bond is observed. The rate enhancement in sulphonic acid buffers is smaller, and a sulphonate base, particularly, is inactive as a catalyst. The rate‐enhancing effect of imidazole is, however, catalytic, and the catalytic inactivity of sulphonate buffers can be attributed to their structure and/or charge. The catalysis by imidazole is a complex system which, in addition to first‐order reactions, involves a process that shows a second‐order dependence in imidazole concentration. The latter reaction becomes significant in acidic imidazole buffers (pH < pK_a), as the buffer concentration increases. The kinetic solvent deuterium isotope effect k_H/k_D, referring to first‐order catalysis by imidazole base, is 2.3 ± 0.3. That referring to second‐order catalysis is most probably much larger, but an accurate value could not be obtained. Copyright © 2007 John Wiley & Sons, Ltd.     

Magnetic calorimeter for registration of small energy release

A scheme of magnetic calorimeter for registration of rare events characterized by small energy release (cosmic rays, WIMPs, solitary X-ray quanta) is proposed. The calorimeter is brought to operation by adiabatic demagnetization, and its magnetic response is measured by a quantum interferometer (SQUID, A. Barone and G. Paterno, Physics and applications of Josephson Effect). Special consideration is given to the specific features of calorimeter operation in the ferromagnetic transition region. The trigger registration of ultrasmall energy release by a ferromagnetic system in the metastable state is described.     

Analyzing the effect of the inventory policy on order and inventory variability with linear control theory

In this paper we apply linear control theory to study the effect of various inventory policies on order and inventory variability, which are key drivers of supply chain performance. In particular, we study a two-echelon supply chain with a stationary demand pattern under the influence of three inventory policies: an inventory-on-hand policy that bases orders on the visible inventory at an installation, an installation-stock policy that bases orders on the inventory position (on-hand plus on-order inventory) at an installation, and an echelon-stock policy that bases orders on the inventory position at that installation and all downstream installations. We prove analytically that the inventory-on-hand policy is unstable in practical settings, confirming analytically what has been observed in experimental settings and in practice. We also prove that the installation-stock and echelon-stock policies are stable and analyze their effect on order and inventory fluctuation. Specifically, we show the general superiority of the echelon-stock in our setting and demonstrate analytically the effect of forecasting parameters on order and inventory fluctuations, confirming the results in other research.     

The silicon effect on the regioselectivity of the Tsuji-Trost reaction. Experimental and theoretical approaches

Regioselectivity of the Tsuji-Trost reaction on allyl acetates and carbonates substituted with silyl groups at the olefinic moiety has been analyzed. Silicon atom in the central carbon atom increases the stability of the intermediate π-allylpalladium cation with respect to the isomeric π-allylpalladium cation featuring the silicon in a terminal carbon atom.     

氯代5-氟脲嘧啶卟啉的红外光谱特性的研究

本文对新合成的对位及间位两类氯代苯基 5 氟脲嘧啶卟啉的红外光谱吸收峰进行了归属和总结 ,讨论了其红外吸收频率随取代基位置变化的规律。指出了苯环上的取代基为电负性强的基团时 ,由于场效应的存在 ,使被测化合物的羰基伸缩振动吸收峰的相对强度发生改变。同时 ,表明了嘧啶环上N原子发生了取代 ,形成单、双取代 5 氟脲嘧啶卟啉化合物的红外光谱特性。     

Micellar effect in hydroformylation of high olefin catalysed by water-soluble rhodium complexes associated with sulfonated diphosphines

High linear alkenes (1-octene and 1-decene) have been hydroformylated using water-soluble rhodium complexes associated with sulfonated diphosphines in the presence of ionic surfactants or methanol. In all cases, the hydroformylation activities were higher than in experiments without additives. The selectivity in aldehydes was higher when we used cetyltrimethylammonium hydrogensulfate (CTAHSO₄) as the surfactant or methanol as the co-solvent.     

Lattice Boltzmann方法模拟多孔介质Klinkenberg效应

格子Boltzmann数值模拟方法是研究复杂的多孔介质结构特别是Klinkenberg效应的有效方法之一，对处理复杂边值问题尤其有效，用格子Boltzmann方法研究了气流穿越多孔介质问题，并将数值计算结果与实验结果进行了比较，结果表明格子Boltzmann方法是数值模拟气流穿越多孔介质问题的有效方法之一。     

含腔电大尺寸导体目标电磁散射的一体化数值模拟

应用等效原理，通过引入口面上等效磁流将含腔导电目标电磁散射简化为腔内、外两个等效 问题. 腔内问题分段求解并应用级联法获得口面等效导纳矩阵；腔内外的耦合关系应用近似 边界元方法描述并由此获得口面等效磁流；最后，这一具有混合源的腔体内外一体化散射问 题则应用所提出的广义混合场积分方程方法建立电磁模型，并用多层快速多极子方法实现高 效数值求解. 实例计算结果与测试结果具有很好的一致性. 关键词： 含腔目标 电磁散射 混合场积分方程 数值分析     

激光介质的多次吸收不均匀内热源模型

针对激光介质的内热源,以多次吸收作用为基础提出了适用于侧面双向泵浦板状激光介质的不均匀内热源模型,即多次吸收不均匀内热源模型,并与均匀内热源和一次吸收内热源模型进行了对比分析。研究表明,介质厚度及光吸收系数组成的无量纲参数不同时,三种内热源模型得到的内热源分布也有所不同。最后给出了内热源不均匀度及多次吸收模型与一次吸收模型之间的差异在不同目标值下的内热源模型适用准则。