Design of a field flow system for the on-line spectrophotometric determination of phosphate, nitrite and nitrate in natural water and wastewater

This study describes the design and optimisation of a field flow system for the in-situ collection and on-line determination of phosphate, nitrate and nitrite by flow injection analysis-spectrophotometry. The method is based on the initial determination of phosphate as its phosphoantimonylmolybdenum blue complex which is then oxidized on-line by nitrite and the decrease in absorbance is monitored at 880 nm. Nitrate is determined as the difference between total and initial nitrite content in a separate flow after reduction to nitrite in a cadmium reductive column. The calibration curves were linear in the range 0–2.00 mg L⁻¹ P-phosphate, 0–10.00 mg L⁻¹ nitrite and 0–7.00 mg L⁻¹ nitrate with correlation coefficients of 0.9979, 0.9993 and 0.9995, respectively. The detection limits, calculated as 3S/N, were 0.15 mg L⁻¹ for P-phosphate, 0.17 mg L⁻¹ for nitrite and 0.09 mg L⁻¹ for nitrate. The reproducibility was below 3.0% (n = 7). Method validation in the analysis of natural water and wastewater samples revealed that it can efficiently be applied to the determination of the target analytes, with recoveries in the range of 92–108%. Correspondence: Athanasios G. Vlessidis, Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, Ioannina 45110, Greece     

初始振动态对氢分子离子电离通道的影响

通过数值求解非Born-Oppenheimer近似条件下的一维含时薛定谔方程,理论模拟了H2+在不同核初始振动态条件下(=0到=7)的库仑爆炸核初始动能释放谱。模拟结果表明：强激光场中氢分子离子的电离通道主要包括直接多光子电离、电荷共振增强电离及有中间过程的电荷共振增强电离,并且可以通过选取不同的初始振动态实现对氢分子离子电离通道的控制。     

Evaluation of the mechanical and morphological properties of long fibre reinforced polyurethane rigid foams

This paper examines the effect of glass fibre reinforcements on the mechanical and morphological properties of polyurethane rigid foams. The processing parameters of the polyurethane foam were maintained constant while the influence of the filler was evaluated in terms of fibre mass content variation (5%, 10% and 20%) and fibre length variation (12.5 mm, 25 mm and 50 mm). Tests were carried out in compression, three-point bending, tension and shear for all material configurations, the variation in fibre mass content having a larger influence on mechanical properties than fibre length. The structure of the specimens was investigated using Scanning Electron Microscopy and Computer Tomography in order to investigate the filler influence on morphology and the scatter in results.     

一类具有直接和间接传播的SIV传染病模型

传染病的传播途径是多种多样的,如:直接传播和间接传播等.在以往的传染病模型中,大多是只考虑直接传播对疾病的影响,而忽略了环境作用对疾病的影响,即间接传播的影响.其实,间接传播在传染病的传播中同样起着重要的作用,忽略间接传播的影响会低估基本再生数,从而错估传染病爆发的规模,给人类的健康带来更大的威胁.研究了一个既有直接传播又有间接传播的SⅣ传染病模型,通过研究模型的动力学行为,给出了无病平衡点和地方病平衡点全局渐近稳定的条件,并用数值模拟证实了该结果.     

时滞速度反馈作用下弹性梁的主共振分析

采用时滞速度反馈控制策略对轴力作用下的弹性梁进行振动控制.根据Newton第二定律建立压电耦合弹性梁的非线性振动控制模型,运用直接法得到时滞反馈作用下弹性梁主共振的一阶近似解,得出系统响应与控制参数的关系.结果表明,主共振的响应存在多解和跳跃现象,调节控制增益和时滞值可以有效抑制大幅振动.     

Fast‐Track,One‐Step E. coli Detection: A Miniaturized Hydrogel Array Permits Specific Direct PCR and DNA Hybridization while Amplification

A timesaving and convenient method for bacterial detection based on one‐step, one‐tube deoxyribonucleic acid (DNA) hybridization on hydrogel array while target gene amplification is described. The hydrogel array is generated by a fast one‐pot synthesis, where N,N′‐dimethylacrylamide/polyethyleneglycol(PEG₁₉₀₀)‐bisacrylamide mixture polymerizes via radical photoinitiation by visible light within 20 min concomitant with in situ capture probe immobilization. These DNA‐functionalized hydrogel droplets arrayed on a planar glass surface are placed in the polymerase chain reaction (PCR) mixture during the thermal amplification cycles. The bacterial cells can be implemented in a direct PCR reaction, omitting the need for prior template DNA extraction. The resulting fluorescence signal is immediately detectable after the end of the PCR (1 h) following one short washing step by microscopy. Therefore a valid signal can be reached within 1.5 h including 10 min for pipetting and placement of the tubes and chips. The performance of this novel hydrogel DNA array was successfully proven with varying cell numbers down to a limit of 10¹Escherichia coli cells.

     

流动注射化学发光法测定醋氯芬酸

基于酸性条件下醋氯芬酸对高锰酸钾氧化甲醛产生微弱化学发光反应体系具有很强的增敏作用,且增强的发光强度在一定范围内与醋氯芬酸浓度呈良好的线性关系,结合流动注射分析技术,建立了测定醋氯芬酸的化学发光新方法。该法测定醋氯芬酸的线性范围为0.08~5.0 mg/L,检出限(3σ)为3.0×10-2mg/L,相对标准偏差(RSD)为1.7%(n=11,c=0.50 mg/L),回收率为98.1%~104.6%。该法已用于片剂和胶囊中醋氯芬酸含量的测定。     

Screening anaphylactic components of MaiLuoNing injection by using rat basophilic leukemia‐2H3 cell membrane chromatography coupled with HPLC–ESI‐TOF‐MS

MaiLuoNing injection is a traditional Chinese medicine that used clinically since the 1950s in China. However, anaphylactic reactions, through the potentiation of mast cell degranulation, have been reported. In the present study, a rat basophilic leukemia‐2H3 cell membrane chromatography coupled with high‐performance liquid chromatography and electrospray ionization‐ion trap‐time of flight‐mass spectrometry method was established for screening, analyzing, and identifying the potential anaphylactic components of MaiLuoNing injection. Harpagoside, a potential degranulator of rat basophilic leukemia‐2H3 cells, was retained in rat basophilic leukemia‐2H3 cell membrane chromatography. We aimed to evaluate the retained components to determine which of those were capable of inducing degranulation of basophilic leukemia cells. A β‐hexosaminidase assay revealed that harpagoside can induce rat basophilic leukemia‐2H3 cell degranulation in a dose‐dependent manner. BLBA/c mice also exhibit passive cutaneous anaphylaxis in response to harpagoside. These results indicate that rat basophilic leukemia‐2H3 cell membrane chromatography coupled with high‐performance liquid chromatography and electrospray ionization ion trap time‐of‐flight mass spectrometry is effective in screening for the anaphylactic components of MaiLuoNing injection.     

基于遗传算法和神经网络的注塑工艺参数优化

基于Moldflow模拟仿真的结果,结合GA算法优化BP网络的结构,建立了模具温度,熔体温度,保压压力,注射速度等工艺参数与塑件体积收缩率的BP网络模型.获得了最优的工艺参数组合,同时预测结果与实际结果吻合.通过神经网络算法(BP)预测注塑工艺参数对塑件质量的影响,可以有效降低其他建模方法的难度和工作量,方法可以推广到塑件其他质量预测过程中.     

人民币汇率变动对我国开放经济中外资流人影响的研究

就人民币汇率变动对我国开放经济外资流入的传导机制进行理论分析,并以高开放地区和行业为代表,结合实际数据利用面板模型进行实证研究,结果表明:人民币汇率与我国开放经济中外资流入呈现反向变动.由于外资对我国开放经济稳定健康发展具有重大意义和影响.因此,我国需保持人民币汇率在中长期稳定,从而避免对外资流入产生大的冲击.