Use of Luminescence Spectroscopy in Clinical and Biological Analysis

Analysis of a sample of clinical or biological origin often requires assay procedures that are rapid, sensitive, selective, and permit the use of microsamples. These constraints have prompted the clinical chemist and analytical biochemist to use many modern tools of the analytical chemist—including atomic absorption spectroscopy, gas chromatography, and luminescence spectroscopy. This review considers the impact of molecular luminescence spectroscopy (fluorescence and phosphorescence) in the analysis of samples of clinical and biological interest. We attempt to outline those procedures and applications of luminescence spectroscopy which are well-established and also consider the promising new developments which should permit wide levels of acceptance in the near future.     

Using components of mathematical ability for initial development and identification of mathematically promising students

Kruteskii's work on the mathematical abilities of school children is a seminal work on the nature of mathematical ability. However, the task of developing methods for the practical application of his work is still a significant problem in mathematics education. The authors have developed a practical application of Kruteskii's approach to the important problem of initially developing components of mathematical ability in student and thereafter identifying mathematically promising students. Examples of problems that were designed to develop ability to generalize, flexibility and reversibility of mental processes are presented. A practical guide for determining the level of development of components of mathematical abilities in individual students, in terms of specified observables, is presented as a set of structured reference tables. The authors set out a practical application protocol that combines use of the tables and sets of specially developed problems for initial development of mathematical abilities prior to identification of mathematically promising students in the general classroom. A significant motivation for this work is the desire to avoid time-consuming and resource intensive practices such as interviews and summer schools which therefore have been used successfully because these practices are now out of reach for all but very wealthy countries or highly ideologically driven systems. On the other hand, special examinations heavily depend on the level of preparedness of the students for the particular examination, and therefore some students with high abilities but with fewer opportunities to prepare could be overlooked.     

湖南生产服务业与先进装备制造业产业发展关系研究

以投入产出模型为基础,计算出湖南省生产服务业与先进装备制造业这两行业与各分行业的直接消耗系数、完全消耗系数、感应度系数和影响力系数.研究表明：先进装备制造业消耗力度大于生产服务业,但二者消耗水平均远低于发达国家,先进装备制造业对生产服务业的需求推动力和影响力较强,生产服务业的传统服务部门对产业发展推力较强,但资本、知识密集型生产服务部门作用正日益凸显,创新型部门则有待进一步发展.基于上述结论,提出了以生产服务业促进先进装备制造业发展,以先进装备制造业推动生产服务业发展的政策建议.     

基于“又好又快发展”思想的动态综合评价方法

针对不同时段内被评价对象增益的不同,提出了一种基于"又好又快发展"思想的动态综合评价方法.首先依据时序立体数据信息,求出被评价对象的增益水平;然后通过均值相对运算、规范化和一致性运算,定义出各被评价对象各指标在某一时刻的相对发展增益权、均衡发展增益权和可持续发展增益权,并进一步计算综合增益权;最后依据激励思想给出增益权与静态综合评价值的合成规则.通过2006-2010年中部六省三次产业"又好又快发展"水平评价的实例分析,证明了方法的科学性.     

A problem structuring method for ecosystem-based management: The DPSIR modelling process

The purpose of this paper is to learn from Complex Adaptive Systems (CAS) theory to inform the development of Problem Structuring Methods (PSMs) both in general and in the specific context of marine management. The focus on marine management is important because it is concerned with a CAS (formed through the interconnection between natural systems, designed systems and social systems) which exemplifies their particularly ‘wicked’ nature. Recognition of this compels us to take seriously the need to develop tools for knowledge elicitation and structuring which meet the demands of CAS. In marine management, chief among those tools is the DPSIR (Drivers – Pressures – State Changes – Impacts – Responses) model and, although widely applied, the extent to which it is appropriate for dealing with the demands of a CAS is questionable. Such questioning is particularly pertinent in the context of the marine environment where there is a need to not only recognise a broad range of stakeholders (a question of boundary critique) but also to manage competing knowledge (economic, local and scientific) and value claims. Hence this paper emphasises how a CAS perspective might add impetus to the development of a critical perspective on DPSIR and PSM theory and practice to promote a more systemic view of decision-making and policy development.     

Designing and scaling up an innovation in abstract algebra

In this paper, we describe the process of designing and scaling up the TAAFU group theory curriculum. This work unfolded in three overlapping stages of research and design. The initial designs emerged along with local instructional theories as the result of small-scale design experiments conducted with pairs of students. A second stage of the research and design process focused on generalizing from the initial laboratory design context to an authentic classroom setting. The third (ongoing) stage involves generalizing to instructors (mathematicians) who were not involved in the design process. We describe each of these stages, and our efforts to investigate the efficacy of the resulting curriculum, in order to provide an illustrative example of the process of scaling up an innovation.     

Deciphering key proteins of oil palm (Elaeis guineensis Jacq.) fruit mesocarp development by proteomics and chemometrics

Palm oil is an edible vegetable oil derived from lipid‐rich fleshy mesocarp tissue of oil palm (Elaeis guineensis Jacq.) fruit and is of global economic and nutritional relevance. While the understanding of oil biosynthesis in plants is improving, the fundamentals of oil biosynthesis in oil palm still require further investigations. To gain insight into the systemic mechanisms that govern oil synthesis during oil palm fruit ripening, the proteomics approach combining gel‐based electrophoresis and mass spectrometry was used to profile protein changes and classify the patterns of protein accumulation during these complex physiological processes. Protein profiles from different stages of fruit ripening at 10, 12, 14, 15, 16, 18 and 20 weeks after anthesis (WAA) were analysed by two‐dimensional gel electrophoresis (2DE). The proteome data were then visualised using a multivariate statistical analysis of principal component analysis (PCA) to get an overview of the proteome changes during the development of oil palm mesocarp. A total of 68 differentially expressed protein spots were successfully identified by matrix‐assisted laser desorption/ionisation‐time of flight (MALDI‐TOF/TOF) and functionally classified using ontology analysis. Proteins related to lipid production, energy, secondary metabolites and amino acid metabolism are the most significantly changed proteins during fruit development representing potential candidates for oil yield improvement endeavors. Data are available via ProteomeXchange with identifier PXD009579. This study provides important proteome information for protein regulation during oil palm fruit ripening and oil synthesis.     

阵列光镊的发展与应用

在生命科学研究中和在微量液体环境下分离液体中的细胞、生物大分子或胶体颗粒一直是一项具有挑战性的工作。"光镊"技术自20世纪80年代被提出到现在,在生命科学研究领域已经得到了日益广泛的运用。激光对细胞捕获的作用已得到进一步扩展,二维"光镊阵列"技术是近年来"光镊"技术中最重要的发展之一。讨论了阵列光镊的发展现状及基本原理,分析了它在生命科学中的应用,并对其发展趋势进行了展望。