菌紫质在银膜表面的共振增强Raman光谱

本文用表面共振增强Raman方法,研究了菌紫质在不同条件下吸附在镀银玻璃上的Raman光谱,结果表明菌紫质吸附在银膜上,代表菌紫质分子循环中间体M412特征谱带的1565cm-1Raman位移明显增强,说明银膜对M412态有一定表面共振增强作用。     

细菌视紫红质光开关特性实验研究

介绍实现光子开关材料BR分子的重要性。理论上分析BR分子材料光学特性，实验观测BR分子材料在400nm和632nm光照射下的相互抑制作用，最后分析BR的光子开关特性。     

嗜盐古菌Haloterrigena thermotolerans JCM 11050Tbop基因序列研究

对标准菌株Haloterrigena thermotolerans JCM 11050^T，其编码螺旋C至螺旋G的细菌视紫红质（bacteriorhodopsin，BR）蛋白基因（bop基因）片断采用PCR方法进行了扩增，TA克隆并测定了其核苷酸序列．对翻译出的氨基酸序列进行亲水性分析，表明该菌株的BR蛋白与已报道相应序列具有类似的二级结构．蛋白序列聚合比对结果表明，该菌株BR蛋白与Natrinema属的BR蛋白具有一定相似性．在这一Haloterrigena属的标准菌株中证实bop基因，进一步丰富了bop基因资源．     

细菌视紫红质／聚乙烯醇复合膜的制备及相关功能研究

细菌视紫红质（bR)是一种独特的光敏蛋白，具有光致变色和光驱质子泵功能 。将bR蛋白包埋于聚乙烯醇（PVA）基质中，制备了bR/PVA复合膜。利用紫外－可 见分光光度计和自制的毫秒级动力学光谱仪，检测了样品的吸收光谱和光循环M中 间体在脉冲光激发下随时间的变化；同时，利用凝胶扫描成像仪及相关分析软件考 察了样品成膜后的均匀程度。实验表明：bR/PVA复合膜具有良好的均匀性、透明性 和力学性能，而且bR蛋白保持了原有的生物活性和光学性质，bR与M中间体之间能 达到一种光可控制的双稳态，M中间体的寿命也得到了显著的延长，证实了bR可以 提供一个用于信息存储的模型材料。     

Two orientations and photochromism of polyelectrolyte/bacteriorhodopsin alternative deposition multilayers

A series of organized multilayers have been formed by the alternative adsorption of positively charged poly(dimethyldiallylammonium chloride) (PDAC) and purple membrane (PM) fragments in suspensions at pH = 4—11. Both UV-vis spectrophotometry and quartz crystal micro-balance (QCM) technique were used to monitor the deposition process of PDAC/bacteriorhodopsin (bR) multilayers, suggesting that PM fragments and PDAC are deposited alternatively on the substrate uniformly. Upon illumination, all these multilayers generate photovoltages with defined signs. The negative sign of photovoltage accompanying the formation of M-state at pH <7 indicates that the extracellular side of PM fragments is directed toward the substrate; and the positive sign at pH≥7 indicates that the cytoplasmic side of PM fragments is directed toward the substrate. In addition, the long-lived multiple M-state has been observed in all multilayer films. Moreover, M-state at high pH, which shows the longer lifetime than that at low pH, de     

Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane

The steady absorption and kinetic changes of M₄₁₂ intermediate of the light- and dark-adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X-100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X-100. The destruction to the cooperative effect of BR ultimately caused 13-cis-retinal of the dark-adapted BR to take part in BR photocycle and also to generate the deprotonated M₄₁₂ intermediate. Project supported by the key and major projects from the Chinese Academy of Sciences (Grant Nos. Kj951-A1-501-05 and Kj952-S1-03)     

Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane

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基于菌紫质薄膜的相移器及其应用

设计了一种基于菌紫质光致各向异性的相移器,并把它用于相移干涉计量。取向随机分布的极性菌紫质分子对线偏振诱导光的选择性吸收导致分子取向分布不均匀,使其呈现宏观的各向异性,这种各向异性与诱导光的偏振特性密切相关,圆偏振光经过各向异性的菌紫质薄膜后,出射光的偏振特性完全由偏振诱导光决定。基于上述原理设计了一种新型的相移器,用琼斯矩阵法推导了基于相移器的相移干涉原理。该相移器在工作过程中不需要移动Mach-Zender干涉仪内部的任何器件,仅需要改变外部控制光路中诱导光的偏振取向就可以控制参考光的相位,有助于提高设备的抗振能力。用最小二乘法对相移干涉结果进行重建,得到了和实际相位一致的结果,验证了相移器的可行性。     

The study of terbium regenerated bacterirhodopsin

Bacteriorhodopsin (bR) is the only retinal-contain- ing protein in the purple membrane of Halobacterium halobium[1]. Upon illumination, the protein undergoes a photocycle and pumps protons across the cell mem-brane[2,3]. It has been found that well-washed…     

基于菌紫质膜联合子波变换的实时模式识别

菌紫质是一种结构和功能与高级动物视网的视生色素－－视紫红质极为相似的蛋白南，是一种优异的可逆光全息记录材料，子波变换是目标特征抽取和模式识别和有效方法。本文钭联合子波变换和菌紫质膜优异的光学全息记录特性结合起来，提出了一种全新的光学全息实时模式识别方案，给出了相应的实验结果。