液相色谱-串联质谱法检测干血点中的同型半胱氨酸

建立了一种高通量液相色谱-串联质谱技术检测干血点(DBS)中同型半胱氨酸(homocycteine, Hcy)的方法。以DBS为样本,homocystine-D8为同位素内标,二硫苏糖醇(DTT)为蛋白结合态Hcy的还原剂,使用含0.1%(v/v)甲酸、0.05%(v/v)三氟乙酸的乙腈溶液萃取。整个前处理过程使用自动移液平台及96孔板实现高通量自动化操作。处理后的样本经过Phenomenex CN柱分离,使用多反应监测模式进行LC-MS/MS分析。结果表明:Hcy的检出限为0.12 μ mol/L(S/N=3),定量限为0.46 μ mol/L(S/N=10)。Hcy在1.16~148.00 μ mol/L范围内线性关系良好,R²=0.994。Hcy的平均回收率为(103.0±4.97)%~(112.0±2.13)%,日内相对标准偏差(RSD)为1.9%~4.6%,日间RSD为1.5%~7.1%。DBS样本在不同温度(-4、-20、22和37℃)下储存不同时间(0、1、2、3、4、5、6、14天)后的稳定性试验显示样本总体RSD<15%,经前处理后的样本在48 h内的稳定性试验显示样本总体RSD<5%。该方法与传统生化分析方法的相关性好(R²=0.9818, n=47)。     

口腔种植修复和常规修复在牙列缺损治疗中的效果观察

目的探讨牙列缺损采用口腔种植修复和常规修复临床对比情况。方法选取广东医学院附属厚街医院口腔科2014年5月至2015年5月收治,采用数字表抽取法随机分组,就常规修复(常规组,n=50)与种植修复(种植组,n=50)效果进行对比。结果观察组总有效率为92%,显著高于对照组70%(P0.05);观察组满意度各因子评分及总评分均高于对照组(P0.05)。结论牙列缺损采用口腔种植修复治疗,可显著提高临床效果,促患者咀嚼、发音等功能恢复,且美观度较理想,有较高推广应用价值。     

An occlusion-adaptive tracker based on sparse representation using alternating direction method of multipliers

Recently, sparse representation has been applied to visual tracking with satisfactory performance. However, partial occlusion and computational complexity are two main obstructions in developing sparse-based tracking. In this paper, a simple yet robust tracker based on patch-based sparse representation is proposed. An adaptive motion model, including adaptive sampling regions and adaptive particle numbers, is proposed to improve the sampling efficiency. A self-adjustable segmentation approach is proposed to segment the target into local patches. A patch-based observation model, which is occlusion-adaptive, is constructed by solving a set of L1-regularized least squares problems. The L1-regularized least squares problem is solved using the alternating direction method of multipliers (ADMM). Both quantitative and qualitative experiments are conducted on several challenging image sequences and the comparisons with several state-of-the-art trackers demonstrate the effectiveness and efficiency of our tracker.     

基于压缩感知的振动数据修复方法

为解决旋转机械振动信号丢失数据修复的问题,提出一种基于压缩感知原理的振动数据修复方法.首先对采集到的不完整信号进行处理,将无信息输入时刻对应的数据用零元素填充得到有损信号,以单位矩阵为基础,根据有损信号中零元素的位置信息,构造对应于压缩感知框架下的观测矩阵;再根据待修复信号的特点并结合先验知识,构造或选择能够对振动信号进行稀疏表示的字典矩阵;然后使用高效且稳定的追踪算法,根据有损信号、观测矩阵以及字典矩阵重构原始信号,实现丢失振动数据的修复.使用仿真数据检验方法的有效性;使用实测的轴承振动状态数据验证方法对于振动数据的适用性,并通过比较完整信号、有损信号和修复信号对应的时域和频域特征值来检验数据修复效果.实验结果表明:本文方法能够有效地实现丢失数据的修复,且从统计特征的角度来看,相比于有损信号,修复信号能够更为准确地描述真实完整的振动信号.     

基于天文观测的相机标定及姿态测量技术研究

为利用相机进行天文观测以实现高精度的相机姿态测量,必须先对相机参数进行精确标定。针对传统相机标定方法工作距离有限的问题,提出了以恒星为控制点的相机标定方法,根据球面天文学方法计算观测时刻控制点的世界坐标,利用摄像测量原理建立了恒星观测模型,求解相机的内外参数并分析了误差因素。实验结果表明,该标定方法在不依赖于精密、复杂的外部设备情况下可达到较高精度,并具有较强的抗噪声能力。将标定结果用于天文观测并求解相机姿态,航向角和俯仰角的解算重复性优于10″,能够满足高精度姿态测量的需求。该方法可进一步推广应用于星敏感器的标定。     

The Analysis of Cell Contents of Some Papaveraceous Plants by Newly Devised Automated Histochemical Chromatography

Abstract

Contents of the colored cells (alkaloidal cells¹) found in Macleaya cordata root and Sanguinaria canadensis rhizome were analysed quantitatively by histochemical chromatography (HC) with the help of newly modified automated micromanipulator.

These were found to have 4 alkaloids viz protopine type protopine(1) and allocryptopine(2) and benzo- [c]phenanthridine type (sanguinarine(3) and cheleryth-rine(4)) in both the plants. Quantitatively the pro-topine alkaloids were 10-40 ng per cell while the benzo-[c]phenanthridines (as chlorides) were only 1-6 ng per cell in M. cordata root. However, in S. canadensis rhizome the relative quantities of the 4 alkaloids (1), (2), (3) and (4) were found to be 10, 13, 137 and 68 ng per cell respectively.     

STOCHASTIC OPTIMAL VIBRATION CONTROL OF PARTIALLY OBSERVABLE NONLINEAR QUASI HAMILTONIAN SYSTEMS WITH ACTUATOR SATURATION

An optimal vibration control strategy for partially observable nonlinear quasi Hamiltonian systems with actuator saturation is proposed. First,a controlled partially observable non-linear system is converted into a completely observable linear control system of finite dimension based on the theorem due to Charalambous and Elliott. Then the partially averaged It stochastic differential equations and dynamical programming equation associated with the completely observable linear system are derived by using the stochastic averaging method and stochastic dynamical programming principle,respectively. The optimal control law is obtained from solving the final dynamical programming equation. The results show that the proposed control strategy has high control effectiveness and control effciency.     

Galileo与GPS相位组合观测值的模型

在介绍Galileo系统空间信号的基础上,以模糊度保持整数为前提,在前人的基础上给出了Galileo系统的四个频率载波与GPS L2载波的组合相位观测值的定义,分析了组合观测值的误差传播规律.最后根据一定的组合标准论述了具有相应特性的组合观测值,并给出一些典型的组合.     

Effect of Cyclic Deformation on Magnetorheological Elastomers

Fatigue properties of magnetorheological elastomer (MRE) samples were investigated based on cis-polybutadiene rubber by using a fatigue test machine. Three MRE samples with iron particles mass fraction of 60%, 70%, and 80% were fabricated, and their properties dependence of three strain amplitudes (50%, 75%, and 100%) were measured. The absolute magnetorheological (MR) effect, storage modulus, and loss modulus of MRE samples after fatigue were evaluated by a modified dynamic mechanical analyzer. The results revealed that MR effect, storage modulus, and loss modulus of MREs containing 80% iron particles depended strongly on the strain amplitudes and the number of cycles, while storage mod-ulus and loss modulus of MREs containing 70% iron particles also depended on the strain amplitudes and the number of cycles but not as strongly as sample which contains 80% iron particles, but the properties of MREs containing 60% iron particles after cyclic deforma-tion were almost independent of the fatigued conditions. In order to investigate the fatigue mechanism of MREs, the sample was carried out with a quasi-static tensile testing and its surface morphology during testing was observed in situ by scanning electron microscopy.     

石油基电流变液中链结构的显微观察

利用显微观察技术连续记录了直流与交流电场下石油基电流变液中的显微结构变化,比较了两种电场下成链、链生长、断链的差异,这种差异包括颗粒的运动、链的形状以及断链时有无崩裂现象,并且分析了造成这种差异的原因,认为交流场下出现的链崩裂现象是由约翰逊-拉贝克效应造成的,通过石油基电流变液在直流、20Hz、50Hz三种电场条件下显微链结构的对比,得出结论横跨两极的链总的横截面积是影响剪应力大小的一个重要因素。