Luminescent Single-Molecule Magnets as Dual Magneto-Optical Molecular Thermometers

Luminescent thermometry allows the remote detection of the temperature and holds great potential in future technological applications in which conventional systems could not operate. Complementary approaches to measuring the temperature aiming to enhance the thermal sensitivity would however represent a decisive step forward. For the first time, we demonstrate the proof-of-concept that luminescence thermometry could be associated with a complementary temperature readout related to a different property. Namely, we propose to take advantage of the temperature dependence of both magnetic (canonical susceptibility and relaxation time) and luminescence features (emission intensity) found in Single-Molecule Magnets (SMM) to develop original dual magneto-optical molecular thermometers to conciliate high-performance SMM and Boltzmann-type luminescence thermometry. We highlight this integrative approach to concurrent luminescent and magnetic thermometry using an air-stable benchmark SMM [Dy(bbpen)Cl] (H₂bbpen=N,N′-bis(2-hydroxybenzyl)-N,N′-bis(2-methylpyridyl)ethyl-enediamine)) exhibiting Dy³⁺ luminescence. The synergy between multiparametric magneto-optical readouts and multiple linear regression makes possible a 10-fold improvement in the relative thermal sensitivity of the thermometer over the whole temperature range, compared with the values obtained with the single optical or magnetic devices.     

Self-quenched Fluorophore Dimers for DNA-PAINT and STED Microscopy

Super-resolution techniques like single-molecule localisation microscopy (SMLM) and stimulated emission depletion (STED) microscopy have been extended by the use of non-covalent, weak affinity-based transient labelling systems. DNA-based hybrid systems are a prominent example among these transient labelling systems, offering excellent opportunities for multi-target fluorescence imaging. However, these techniques suffer from higher background relative to covalently bound fluorophores, originating from unbound fluorophore-labelled single-stranded oligonucleotides. Here, we introduce short-distance self-quenching in fluorophore dimers as an efficient mechanism to reduce background fluorescence signal, while at the same time increasing the photon budget in the bound state by almost 2-fold. We characterise the optical and thermodynamic properties of fluorophore-dimer single-stranded DNA, and show super-resolution imaging applications with STED and SMLM with increased spatial resolution and reduced background.     

理解和应用疏水相互作用的尺度依赖原理

Lum,Chandler 和Week 三人建立起来的尺度依赖的疏水相互作用理论为人们提供了一个 重要的理论框架（LCW 理论）去理解和进一步研究与疏水相互作用相关的现象。按照LCW 理 论,当一个疏水粒子的半径小于1 纳米,它的疏水水合自由能与其半径的三次方成线性关系;而当 一个疏水粒子的半径大于1 纳米,它的疏水水合自由能与其半径的二次方成线性关系。1 纳米是一 个较为普适的转变半径。小于这个尺度,水合的热力学过程是由熵主导的,大于这个尺度,水合的 热力学过程是由焓主导的。在这篇综述里,我们介绍了温度,压强和水里面的添加物对疏水水合自 由能的影响。在实验上,我们对基于原子力显微镜的单分子力谱方法研究疏水高分子水合能作了重 点介绍。同时,对这一理论在蛋白质折叠和膜组装等体系中的应用也做了介绍。     

Bi-2212超导线材研究进展

Bi-2212高温超导材料在高磁场中具有优异的磁场载流能力,可制备成各向同性圆线,可以绞制成大电流的电缆导体,是大型强磁场磁体的首选材料之一.经过30多年的发展,Bi-2212超导线材的临界工程电流密度(JE)也已达到工程应用要求.综述了国内外近年来Bi-2212超导材料在载流性能、绝缘、内插线圈和热处理等方面的研究进...     

单块磁悬浮系统磁体和超导体的厚度优化

研究了磁铁厚度和YBCO超导体厚度对悬浮力的大小及系统动态特性的影响,通过分析得出磁悬浮系统中磁体和超导体厚度的最优值。通过悬浮力测试系统测得超导块尺寸为φ30mm×18mm时四块不同厚度的磁体的悬浮力以及磁体尺寸为φ30mm×10mm时六块不同厚度的YBCO超导体的悬浮力的大小;并在振动实验台上测试了上述情况下的振动特性。对动态曲线进行频谱分析,最终得出最优组合,从而对磁悬浮系统的磁体和超导体厚度进行了优化。     

合肥同步辐射光源波动器UD-1物理设计

 介绍了国家同步辐射实验室二期工程新建波动器UD-1的物理设计，给出了设计参数和主要技术要求。从储存环电子束流的要求和用户对光源的要求两方面分析了波动器磁场品质应满足的技术指标，给出UD 1的磁场一次积分值应小于2×10^-4T·m，二次积分值应小于2×10^-4T·m ²,相位误差应小于10°。     

An Engineered OmpG Nanopore with Displayed Peptide Motifs for Single-Molecule Multiplex Protein Detection

Molecular detection via nanopore, achieved by monitoring changes in ionic current arising from analyte interaction with the sensor pore, is a promising technology for multiplex sensing development. Outer Membrane Protein G (OmpG), a monomeric porin possessing seven functionalizable loops, has been reported as an effective sensing platform for selective protein detection. Using flow cytometry to screen unfavorable constructs, we identified two OmpG nanopores with unique peptide motifs displayed in either loop 3 or 6, which also exhibited distinct analyte signals in single-channel current recordings. We exploited these motif-displaying loops concurrently to facilitate single-molecule multiplex protein detection in a mixture. We additionally report a strategy to increase sensor sensitivity via avidity motif display. These sensing schemes may be expanded to more sophisticated designs utilizing additional loops to increase multiplicity and sensitivity.     

高场超导磁体科学技术与应用

科学技术的发展对于磁场强度质量的要求越来越高,极端强磁场条件是人类追求的永远的科学目标,它孕育着许多重大的科学发现和新技术的产生,对人类的科学和技术以及生活产生重大的影响.以磁体为核心与电力电子器件以及相关的软件等结合可以构成各种各样科学仪器和装置,广泛应用在科学研究和工业特种装备中.磁技术对于人类的科学与技术进步起到越来越重要的作用,尤其是极高磁场所带来的诸多优点,使得人类对于物质世界认识不断加深,对于生命的起源以及从事疾病的防治的研究有特别重要的意义.本文介绍磁体基本原理、磁场产生的方法与应用以及相关的发展.     

GSI收集环二极磁体稳定性分析

收集环二极磁体在探测反质子和离子加速器装置中有着重要的作用.在本文中,通过对收集环二极磁体的绝热理论分析,得出其稳定性参数与运行电流之间的关系.另外运用Miils方程计算得出失超后收集环二极磁体内导体温度与时间的对应关系.