基于连续小波变换FTIR鉴定青葙子及鸡冠子的研究

借助OMNI采样器,应用傅里叶变换红外光谱方法直接、快速、准确地测定青葙子及其伪品鸡冠子的红外光谱。利用连续小波变换对真伪品的红外光谱进行放大处理,以有效突出真伪品间的红外光谱差异程度,从而提高鉴定正确率。采用对信号奇异性具有良好探测能力的Morlet小波做小波母函数,对青葙子真伪品的红外光谱进行若干尺度的一维连续小波变换,在各个尺度下观察青葙子的真伪品的红外光谱的差异程度,从中选择一个差异程度最为明显的尺度来区分青葙子真伪品。结果表明：基于连续小波变换的傅里叶红外光谱分析方法能够有效鉴别同科同属不同种植物中药材。     

常用十字花科药用植物中芥子碱的分布研究

十字花科植物中的芥子碱是一种非常有价值的天然抗氧剂,在抗衰老药物的研究中受到了许多学者的重视。为了考察芥子碱在我国药用植物中的分布情况,采用反相高效液相色谱法测定了我国常用的4种十字花科药用植物白芥子、莱菔子、葶苈子、黄芥子中该成分的含量。采用的色谱柱为Alltima Phenyl柱(250 mm×4.6 mm i.d.,5 μm);流动相:3%醋酸溶液和乙腈的混合溶液,梯度洗脱;检测波长:326 nm;柱温:25 ℃。该法快速,准确度高,重现性好,方法的建立不仅为上述4种药材的质量监测提供了有效的定量分析方法,而且对于芥子碱的深入研究具有重要意义。     

Identification and determination of (+)-sesamin in Semen Cuscutae by capillary GC and GC-MS

(+)-Sesamin has been found to have pharmacological effect which can improve activity, prevent aging and has the effect of antihypertension 1-2. Figure 1 shows its chemical structure. (+)-Sesamin has been known to be abundant in sesame originally, but our previous study shows that (+)-sesamin also presents in a Chinese herbal medicine Semen Cuscutae3-4. Gas chromatography (GC) and gas chromatography - mass spectrometry (GC-MS) have been applied for the determination and identification of (+…     

决明子生芽转化有机铬的效果研究

利用透析方法将不同时间点收获的富铬决明子芽中的有机铬和无机铬分开,用石墨炉原子吸收分光光度法测定富铬决明子芽中的总铬含量和无机铬含量,计算出各时间点收获的决明子芽的有机铬转化率.探讨决明子生芽转化有机铬的效果.培育时间对决明子芽有机铬转化率有一定影响,在本实验条件下,72h是培育富铬决明子的适宜时间段.     

High resolution UPLC-MS/MS method for simultaneous separation and determination of six flavonoids from semen cuscutae extract in rat plasma: application to comparative pharmacokinetic studies in normal and kidney-deficient rats

Abstract

Semen Cuscutae, which mainly consisted of flavonoids, is a traditional Chinese herbal medicine and used for nourishing the liver and kidneys. The aim of this study was to develop a sensitive and selective UPLC-MS/MS method for simultaneous separation and determination of six main active renoprotective components of Hyperoside, Astragalin, Isoquercitrin, Quercitrin, Quercetin, and Kaempferol from Semen Cuscutae in rat plasma, and to reveal the pharmacokinetic differences between normal and kidney deficient rats. The validated method has been successfully applied to comparing pharmacokinetic profiles of the six analytes in rat plasma. The results indicated that there was significant difference in pharmacokinetic parameters of the six analytes between two groups, while absorptions in kidney deficient group were significantly lower than those in normal group. This study would be helpful for evaluating the Semen Cuscutae as renoprotective drug candidates for pre-clinical and clinical research.     

补骨脂及其混伪品蛋白质电泳鉴别

为鉴别补骨脂与其混伪品,对两种种子的可溶性蛋白质进行了聚丙烯酞胺凝胶电泳分析.结果表明,补骨脂及其混伪品的电泳图谱存在显著差异,电泳图谱可作为补骨脂及其混伪品的鉴别依据.     

决明子药材中大黄酚含量的反相HPLC法测定

以甲醇 -水 (体积比80∶20)为流动相,采用反相高效液相色谱法测定了生、炒决明子中大黄酚的含量 ,平均回收率98.1%,RSD为2.3%(n=5)。方法简便、灵敏、重现性好。     

气相色谱/质谱-化学计量学法分析测定药对桃仁-红花挥发油

药对是中药配伍中最基本、最常用的形式,具有中药配伍的基本特点。药对化学是复方化学的核心内容。联用色谱和化学计量学方法是分析中药复方复杂体系的有效工具。采用GC/MS法分离测定了药对桃仁-红花、单味药桃仁和红花的挥发油成分,并对其重叠色谱峰采用化学计量学解析法进行了分辨,得到药对和各单味药的纯色谱曲线和质谱。药对桃仁-红花、单味药桃仁和红花分辨出的色谱峰,通过质谱库对其进行定性,分别得到84、27和52个定性结果,占总含量的92.06%、89.43%和94%。实验结果表明:桃仁-红花挥发油成分与单味药桃仁和红花的存在较大差别,也不是两者挥发油成分之加和。     

Chemical profiling and quantification analysis of flavonoids in different varieties of Euryales Semen by ultra-high-performance liquid chromatography with tandem mass spectrometry

Euryales Semen was a traditional Chinese medicine, which has been commonly used to treat spermatorrhea, enuresis, and frequent urination. Flavonoids were a critical ingredient in determining the function and quality of Euryales Semen. At present, no effective method has been established for the qualitative of Euryales Semen flavonoids. In this study, an ultra-high-performance liquid chromatography-quadrupole-time of flight-mass spectrometry method was established for flavonoids. By comparison with standard or literature data, 32 flavonoid compounds have been identified in Euryales Semen. Based on the qualitative results, an ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectroscopy method was developed for the main components, and the linearity, the limit of detection, limit of quantification, repeatability, precision, stability, and recovery of the method were verified. The principal component analysis and the hierarchical clustering heatmaps analysis showed that the 30 batches of samples were distinctly separated into the North Gordon Euryale and South Gordon Euryale, and the measured contents of the six flavonoids in North Gordon Euryale were more abundant than in South Gordon Euryale, especially isoquercitrin, hesperetin, and quercetin. It provided a scientific basis for the quality control of Euryales Semen and a theoretical basis for the rational utilization of Euryales Semen resources.