Reversible 5′-end biotinylation and affinity purification of synthetic RNA

A reversible biotinylation phosphoramidite was synthesized and incorporated onto the 5′-end of an oligoribonucleotide on a solid phase synthesizer. After cleavage and deprotection, the crude synthetic oligomer mixture was incubated with NeutrAvidin^® coated microspheres, and the failure sequences removed by washing with a buffer followed by treating the microspheres with tetrabutylammonium fluoride to give a high quality unmodified full-length oligoribonucleotide.     

Novel Design of RNA Aptamers as Cancer Inhibitors and Diagnosis Targeting the Tyrosine Kinase Domain of the NT-3 Growth Factor Receptor Using a Computational Sequence-Based Approach

Aptamers, the nucleic acid analogs of antibodies, bind to their target molecules with remarkable specificity and sensitivity, making them promising diagnostic and therapeutic tools. The systematic evolution of ligands by exponential enrichment (SELEX) is time-consuming and expensive. However, regardless of those issues, it is the most used in vitro method for selecting aptamers. Therefore, recent studies have used computational approaches to reduce the time and cost associated with the synthesis and selection of aptamers. In an effort to present the potential of computational techniques in aptamer selection, a simple sequence-based method was used to design a 69-nucleotide long aptamer (mod_09) with a relatively stable structure (with a minimum free energy of −32.2 kcal/mol) and investigate its binding properties to the tyrosine kinase domain of the NT-3 growth factor receptor, for the first time, by employing computational modeling and docking tools.     

Folding Free Energy Determination of an RNA Three-Way Junction Using Fluctuation Theorems

Nonequilibrium work relations and fluctuation theorems permit us to extract equilibrium information from nonequilibrium measurements. They find application in single-molecule pulling experiments where molecular free energies can be determined from irreversible work measurements by using unidirectional (e.g., Jarzynski’s equality) and bidirectional (e.g., Crooks fluctuation theorem and Bennet’s acceptance ratio (BAR)) methods. However, irreversibility and the finite number of pulls limit their applicability: the higher the dissipation, the larger the number of pulls necessary to estimate ΔG within a few kBT. Here, we revisit pulling experiments on an RNA three-way junction (3WJ) that exhibits significant dissipation and work-distribution long tails upon mechanical unfolding. While bidirectional methods are more predictive, unidirectional methods are strongly biased. We also consider a cyclic protocol that combines the forward and reverse work values to increase the statistics of the measurements. For a fixed total experimental time, faster pulling rates permit us to efficiently sample rare events and reduce the bias, compensating for the increased dissipation. This analysis provides a more stringent test of the fluctuation theorem in the large irreversibility regime.     

New Derivatives of 5-Substituted Uracils: Potential Agents with a Wide Spectrum of Biological Activity

Pyrimidine nucleoside analogues are widely used to treat infections caused by the human immunodeficiency virus (HIV) and DNA viruses from the herpes family. It has been shown that 5-substituted uracil derivatives can inhibit HIV-1, herpes family viruses, mycobacteria and other pathogens through various mechanisms. Among the 5-substituted pyrimidine nucleosides, there are not only the classical nucleoside inhibitors of the herpes family viruses, 2′-deoxy-5-iodocytidine and 5-bromovinyl-2′-deoxyuridine, but also derivatives of 1-(benzyl)-5-(phenylamino)uracil, which proved to be non-nucleoside inhibitors of HIV-1 and EBV. It made this modification of nucleoside analogues very promising in connection with the emergence of new viruses and the crisis of drug resistance when the task of creating effective antiviral agents of new types that act on other targets or exhibit activity by other mechanisms is very urgent. In this paper, we present the design, synthesis and primary screening of the biological activity of new nucleoside analogues, namely, 5′-norcarbocyclic derivatives of substituted 5-arylamino- and 5-aryloxyuracils, against RNA viruses.     

连续激光辐照ZnSe/MgF2/K9滤光片的透射特性研究

分析了连续激光辐照ZnSe/MgF2/K9滤光片引起透射特性的变化。在室温条件下,用波长0.632 8μm激光作为探测光束,测量了1.06μm连续激光辐照ZnSe/MgF2/K9滤光片温升引起薄膜折射率的改变,导致探测光束通过干涉滤光片后透过率的热致非线性变化。在光斑直径0.75mm条件下,测量了不同功率激光辐照ZnSe/MgF2/K9滤光片引起温升随时间的变化。在激光功率30W,辐照时间2.52s条件下,实验观测到ZnSe/MgF2/K9滤光片薄膜破坏温度约为90℃,辐照时间10s时干涉滤光片形成的薄膜龟裂形貌。     

Inter-cell spectrum efficiency improvement technology

Improvement in inter-cell spectrum efficiency is a valuable research topic in mobile communication system, which affects cell edge user experience especially. According to current research results, there are three methods to deal with inter-cell interference to improve inter-cell spectrum efficiency, including inter-cell interference randomization, inter-cell interference cancellation and inter-cell interference coordination. This paper analyzes three important inter-cell spectrum efficiency improvement technologies, soft frequency reuse (SFR), uplink power control, and downlink coordinated multi-point transmission/reception (CoMP), and relative research progress.     

Scanning heterodyne reconstruction of coded aperture images

The reconstruction of coded aperture images using an optical scanning heterodyne processor is described for a class of apertures which can be synthesized by two beam interference. The analysis of Fresnel zone apertures indicates that a lateral and longitudinal (tomographic) resolution close to the theoretical limit is achievable. Experimental results of optical simulations confirm this assertion. Advantages of such technique include real-time operation and large multiplexing capability.     

多位数序列全加器的光电混合实现

本文报道用光不双稳开关构成多位数序列全加器的方法，并用非线性干涉滤光片双稳开关进行了原理性实验演示，完成了四位二进制数的全加运算。     

双折射晶体在偏振光杨氏干涉实验中的应用

在正交偏光系统中置入一天然解理的双折射晶块和会聚透镜,可获得清晰的偏振光干涉分布图样。该文利用双折射晶体的偏振分束原理对干涉图样的产生和条纹分布进行具体的分析和计算。