Enzymatic Synthesis of Fructosyl Glycerol

ABSTRACT

The enzymatic synthesis of ß2-2' and ß2-1'-D- fructopyranosyl glycerol was carried out with levansucrase from Bacillus circulans or B.subtilis, using sucrose as fructosyl donor and glycerol. The specificity and efficiency of the enzyme was modified by controlling both the water and the total substrate concentrations. The products were purified by HPLC and analyzed by ¹H, ¹³C NMR and GC-MS.     

The spectrophotometric study of a fluorescence‐enhanced inclusion complex threaded with β‐cyclodextrin: Synthesis and characterization

A novel achiral monomer end‐capped with a phenyl‐[1,3,4]oxadiazolyl group and threaded through β‐cyclodextrin was synthesized to investigate the host‐guest interactions in the inclusion complex. ¹H NMR studies revealed that one or two cyclodextrin molecules were threaded onto the synthesized achiral monomer, leading to the formation of a fibrous construction of self‐assembled inclusion complexes. The formation of a self‐assembled inclusion complex was identified using SEM and TEM. The highly ordered alignment of self‐assembled supramolecules was confirmed using polarized optical microscopy. We demonstrate an easy process for the fabrication of nano‐structured self‐assembled inclusion complexes in pyridine/ethanol (1 mL/10 mL) as well as the enhancement of photo‐induced fluorescence via monomers end‐capped with a phenyl‐[1,3,4]oxadiazolyl moiety threaded with β‐cyclodextrins. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 3368–3374, 2010     

A hyperbranched,rotaxane‐type mechanically interlocked polymer

Based on the dibenzo‐24‐crown‐8/1,2‐bis(pyridinium)ethane recognition motif, a hyperbranched mechanically interlocked polymer was prepared by polyesterification of an easily available dynamic trifunctional AB₂ pseudorotaxane monomer. It was characterized by various techniques including ¹H NMR, COSY, NOESY, GPC, viscosity, TGA, dynamic laser light scattering, AFM, and SEM. Its GPC M_n was determined to be 191 kDa with polydispersity 1.7 and its hydrodynamic diameter in a dilute solution in acetone was about 70 nm. This measured M_n value corresponds to about 93 repeating units. The study reported here presents not only a new polymer topology but also a novel and convenient way to prepare mechanically interlocked polymers. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 4067–4073, 2010     

Solving the radiation diffusion and energy balance equations using pseudo-transient continuation

We develop a scheme for the system coupling the radiation diffusion and matter energy balance equations. The method is based on fully implicit, first-order, backward Euler differencing; Picard-Newton iterations solve the nonlinear system. We show that iterating on the radiation energy density and the emission source is more robust. Since the Picard-Newton scheme may not converge for all initial conditions and time steps, pseudo-transient continuation (Ψtc) is introduced. The combined Ψtc-Picard-Newton scheme is analyzed. We derive conditions on the Ψtc parameter that guarantee physically meaningful iterates, e.g., positive energies. Successive Ψtc iterates are bounded and the radiation energy density and emission source tend to equilibrate. The scheme is incorporated into a multiply dimensioned, massively parallel, Eulerian, radiation-hydrodynamic computer program with automatic mesh refinement (AMR). Three examples are presented that exemplify the scheme's performance. (1) The Pomraning test problem that models radiation flow into cold matter. (2) A similar, but more realistic problem simulating the propagation of an ionization front into tenuous hydrogen gas with a Saha model for the equation-of-state. (3) A 2D axisymmetric (R,Z) simulation with real materials featuring jetting, radiatively driven, interacting shocks.     

The almost periodic type difference equations

In this paper, we study the existence of almost periodic type solutions of difference equations by means of exponential dichotomy and trichotomy of linear difference equations.     

一种快速求红移和证认谱线的新方法--伪三角法

对于河外天体，谱线证认和红移确定是光谱分析的第一步。本文在仔细分析天体光谱特点的基础上，提出了一种全新的求红移和证认谱线的新方法--伪三角法。该方法利用最强的三根谱线的波长信息构造“三角形”，通过将最大角的“余弦”与已知模板的“余弦”表相匹配，反推得到相应的标准谱线波长，并进而得到红移值。通过这个红移值，可以得到所有的谱线的标准位置，这就相当于谱线证认。结果表明：这种方法在大大减少运算量的同时，保持了较高的正确识别率和极低的误识率。这对海量光谱数据的处理具有重要的意义。同时，该方法立意新颖，简单明了，容易实现。缺点是对于谱线少于3根的光谱以及噪声很大的光谱不能有效地处理。     

Unsupervised LoS/NLoS identification in mmWave communication using two-stage machine learning framework

Identification of line-of-sight (LoS)/ non-LoS (NLoS) condition in millimeter wave (mmWave) communication is important for localization and unobstructed transmission between a base station (BS) and a user. A sudden obstruction in a link between a BS and a user can result in poorly received signal strength or termination of communication. Channel features obtained by the estimation of channel state information (CSI) of a user at the BS can be used for identifying LoS/NLoS condition. With the assumption of labeled CSI, existing machine learning (ML) methods have achieved satisfactory performance for LoS/NLoS identification. However, in a real communication environment, labeled CSI is not available. In this paper, we propose a two-stage unsupervised ML based LoS/NLoS identification framework to address the lack of labeled data. We conduct experiments for the outdoor scenario by generating data from the NYUSIM simulator. We compare the performance of our method with the supervised deep neural network (SDNN) in terms of accuracy and receiver characteristic curves. The proposed framework can achieve an accuracy of 87.4% and it outperforms SDNN. Further, we compare the performance of our method with other state-of-the-art LoS/NLoS identification schemes in terms of accuracy, recall, precision, and F1-score.     

Guest-dependent complexation of triptycene-derived macrotricyclic host containing one anthracene moiety with paraquat derivatives: construction of [2]rotaxanes

Complexation between the triptycene-derived macrotricyclic polyether containing an anthracene unit and paraquat derivatives in both solution and solid state was investigated. It was found that the macrotricyclic host with multi-cavity structure could form a series of [2]pseudorotaxanes with different terminal functionalised paraquat derivatives in different threading modes, which subsequently resulted in the construction of two novel [2]rotaxanes.     

Encapsulation of Functional Organic Compounds in Nanoglass for Optically Anisotropic Coatings

A novel approach is presented for the encapsulation of organic functional molecules between two sheets of 1 nm thin silicate layers, which like glass are transparent and chemically stable. An ordered heterostructure with organic interlayers strictly alternating with osmotically swelling sodium interlayers can be spontaneously delaminated into double stacks with the organic interlayers sandwiched between two silicate layers. The double stacks show high aspect ratios of >1000 (typical lateral extension 5000 nm, thickness 4.5 nm). This newly developed technique can be used to mask hydrophobic functional molecules and render them completely dispersible in water. The combination of the structural anisotropy of the silicate layers and a preferred orientation of molecules confined in the interlayer space allows polymer nanocomposite films to be cast with a well‐defined orientation of the encapsulated molecules, thus rendering the optical properties of the nanocoatings anisotropic.