丙酮/水混合体系合成聚苯胺的聚合过程研究

对丙酮/水体系中合成聚苯胺(PAn)的反应进行跟踪,通过聚合过程的开路电压、聚合温度、特性粘度和中间体的分子结构对聚合过程进行研究。结果表明,丙酮/水体系对控制产物的粒度和分子量都有利;当丙酮/水的体积比为0.2左右时,所得产物的各项性能较好。与水体系相比,在丙酮/水混合体系中聚合过程维持高电位的时间延长,聚合链增长时间和自加速反应时间也有延长。苯胺聚合是以头-尾连接形式进行,且中间产物由高氧化态逐渐转变成中间氧化态。     

Implementing a two-layer feed-forward catalytic DNA circuit for enzyme-free and colorimetric detection of nucleic acids

In the present study, a highly sensitive and specific bio-sensing platform for enzyme-free and colorimetric detection of nucleic acids has been developed. The biosensor is composed of two DNA nanostructures and two fuel strands that construct the foundation of a feed-forward catalytic DNA circuit. Upon binding the target strand to a specific DNA nanostructure, the circuit is run in order that at the end a hemin-binding aptamer, with the ability to convert a colorless substrate into a colored substance is released. Based on this strategy, 4 pM of the target DNA can be easily detected in serum samples by naked eyes after only a two-hour incubation with the circuit; meanwhile, if the incubation time is extended to 3 h, the biosensor can detect 1 pM of the target DNA. Besides the elevated sensitivity, the circuit can truly discriminate a spurious target containing one nucleotide mismatch with high specificity. Overall, the enzyme-free catalytic DNA circuit can be used as a sensitive alternative method to enzyme-based biosensors for the specific and cost-effective detection of nucleic acids.     

Electrical Properties of HeLa Cells Based on Scalable 3D Interdigital Electrode Array

This study analyzed the electrical properties of a multiple‐cells using the electric cell‐substrate impedance sensing, a scalable three‐dimensional electrode array and an equivalent circuit model (ECM). The experimental results validated the accuracy and validity of the extraction method for the ECM. The ECM simulation results using the electrical properties extracted by measuring 52 HeLa cells successfully forecasted the impedance magnitudes and phases for 15, 29, 78, and 98 HeLa cells. Comparing the ECM simulations and measurements, the maximum average errors in magnitude and phase were 3.06 % and 4.67 %, suggesting the number of HeLa cells can be classified by their electrical properties.     

RLC串联电路充电过程探究

本论文借助于MATLAB软件对RLC串联电路进行了动态分析并分别探究了电阻、电容、电感这三个因子对RLC串联电路充电的影响情况。利用Simulink中的Simpowersystems对RLC串联电路的充电过程进行仿真,探究电阻、电容、电感这三个因子对RLC串联电路充电过程的影响。     

电气原理草图语义理解系统开发

在当前已有的手绘草图识别系统及算法的基础上,设计了一个简单的手绘电气元件识别系统.通过在线采集获取手绘图每个笔画的始终点坐标以及每个笔画上所有的采样点的坐标,然后通过平滑处理以及去重处理来降低因输入时候的抖动噪音之类的因素对识别的影响.实现了基于图元以及图元数量的单个电气元件的识别方法.系统使用MATLAB做为软件开发平台,通过仿真验证算法的可行性,所达到的识别效果能够实现预期的设计要求.     

基于全局灵敏度的电路系统激励与测点优选

针对模拟电路系统级故障诊断问题,提出一种基于全局灵敏度的测试点与测试频率优选方法。利用Pspice软件进行参数分析,避免了电路的拓扑分析和冗繁的计算过程,节省了时间开销,综合考虑元件参数变化影响,定义了网络全局灵敏度的概念,并以此为依据比较不同频率下测点的故障诊断能力,实现测点与测试频率的优选。通过实验仿真验证了方法的有效性,结果表明新方法提高了对系统的故障识别率。     

基于VPX总线的高速数字电路测试系统研究及应用

为解决战略预警雷达、舰载一体化等重点型号雷达中高速数字电路模块的维护能力,设计了基于VPX总线的高速数字电路自动测试系统,通过自研基于VPX总线的多功能测试模块、光纤测试模块,以及通用VPX背板,再结合通用的仪器设备构建测试系统,可兼顾多个型号雷达高速数字电路模块的测试。该系统可提供10路光纤通道,波特率最高为3.2Gbps;16路GPIO信号,中断响应时间＜50us;14路Rocket IO信号,传输速率2.5Gbps;4路*4Rapid IO信号,传输速率3.125Gbps。研究及实测结果表明该系统可解决基于VPX总线的高速数字电路模块的测试。     

Glyco-acrylate copolymers for bilayer tethering on benzophenone-modified substrates

Model biological membranes are becoming increasingly important for studying fundamental biophysical phenomena and developing membrane-based devices. To address the anticipated problem of non-physiological interactions between membrane proteins and substrates seen in “solid-supported lipid bilayers” that are formed directly on hydrophilic substrates, we have developed a polymer-tethered lipid bilayer system based on a random copolymer with multiple lipid analogue anchors and a glyco-acrylate backbone. This system is targeted at applications that, most importantly, require stability and robustness since each copolymer has multiple lipid analogues that insert into the bilayer. We have combined this copolymer with a flexible photochemical coupling scheme that covalently attaches the copolymer to the substrate. The Langmuir isotherms of mixed copolymer/free lipid monolayers measured at the air–water interface indicate that the alkyl chains of the copolymer lipid analogues and the free lipids dominate the film behavior. In addition, no significant phase transitions are seen in the isotherms, while hysteresis experiments confirm that no irreversible states are formed during the monolayer compression. Isobaric creep experiments at the air–water interface and AFM experiments of the transferred monolayer are used to guide processing parameters for creating a fluid, homogeneous bilayer. Bilayer homogeneity and fluidity are monitored using fluorescence microscopy. Continuous bilayers with lateral diffusion coefficients of 0.6 μm²/s for both leaflets of the bilayer are observed for a 5% copolymer system.     

用线性霍耳集成电路测量螺线管中磁场分布

本介绍了一种霍耳集成传感器的特性及测磁场方法,并给出了测量长直螺线管内磁场的实验结果。     

比较逻辑与异步触发时序电路的分析和综合

本文从触发器的完整状态方程出发,研究了异步触发时序电路的分析与综合的新方法,提出了触发器的α跃变、β跃变行为可用比较运算中的小于、大于运算表示,而各触发器的α跃变、β跃变之间的覆盖关系则可用大于等于运算进行检验。在此基础上提出了适用于使用计算机的异步触发时序电路的分析和综合程序。