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111.
S-nitrosylated proteins are biomarkers of oxidative damage in aging and Alzheimer's disease (AD). Here, we report a new method for detecting and quantifying nitrosylated proteins by capillary gel electrophoresis with laser induced fluorescence detection (CGE-LIF). Dylight 488 maleimide was used to specifically label thiol group (SH) after switching the S-nitrosothiol (S-NO) to SH in cysteine using the "fluorescence switch" assay. In vitro nitrosylation model-BSA subjected to S-nitrosoglutathione (GSNO) optimized the labeling reactions and characterized the response of the LIF detector. The method proves to be highly sensitive, detecting 1.3 picomolar (pM) concentration of nitrosothiols in nanograms of proteins, which is the lowest limit of detection of nitrosothiols reported to date. We further demonstrated the direct application of this method in monitoring protein nitrosylation damage in MQ mediated human colon adenocarcinoma cells. The nitrosothiol amounts in MQ treated and untreated cells are 14.8±0.2 and 10.4±0.5 pmol/mg of proteins, respectively. We also depicted nitrosylated protein electrophoretic profiles of brain cerebrum of 5-month-old AD transgenic (Tg) mice model. In Tg mice brain, 15.5±0.4 pmol of nitrosothiols/mg of proteins was quantified while wild type contained 11.7±0.3 pmol/mg proteins. The methodology is validated to quantify low levels of S-nitrosylated protein in complex protein mixtures from both physiological and pathological conditions. 相似文献
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113.
Zhijuan Cao Fengxian Qiu Qing Wang Guorong Cao Lin Zhuang Qiang Shen Xiaolong Xu Jie Wang Qian Chen Dongya Yang 《Optik》2013
The azo benzothiazole polyurethane–urea (ABPUU) was synthesized from chromophore molecule 4-[(6-nitrobenzothiazole-2-yl)diazenyl]phenyl-1,3-diamine NBDPD, polyether polyol (NJ-210) and isophorone diisocyanate (IPDI). The structure, thermal property, mechanical property and physical property were characterized and investigated. The refractive index (n) and thermo-optic coefficient (dn/dT) of ABPUU was determined at different temperature and wavelength (532 nm, 650 nm and 850 nm) using attenuated total reflection (ATR) technique. Using the CCD digital imaging devices, transmission loss of ABPUU was measured. A 1 × 2 Y-branched and 2 × 2 Mach–Zehnder interferometer (MZI) switch with two rib waveguides, dual driving electrodes and two critical 3-dB couplers polymeric thermo-optic switches based on thermo-optic effect of prepared ABPUU were designed and simulation. The power consumption of the Y-branched switch is less than 0.84 mW. The Y-branched and MZI switching rising and falling times obtained are 0.8 ms and 0.2 ms, respectively. 相似文献
114.
本文提出了量子信令交换机的模型, 该交换机由经典信息控制模块、交换控制模块和量子交换模块三部分组成. 经典控制模块负责将纠缠初态信息传送给纠缠测量及交换单元并更新路由信息. 交换控制模块实现通路选择, 为纠缠对的分发提供通路. 量子交换模块制备纠缠对, 进行Bell态的测量, 完成纠缠交换. 量子信令交换机可以实现多用户间的信令传输及局域网通信. 通过对交换机的性能分析与仿真, 结果表明该交换机结构简单、安全保密、便于扩展、时延小, 对于构建量子通信网有很好的支撑作用.
关键词:
量子通信
量子信令网
量子信令交换机
纠缠交换 相似文献
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Spectral changes of vector Gaussian Schell-model beams passing through an aperture lens 总被引:1,自引:0,他引:1
On the basis of the polarization matrix and propagation equation of the cross-spectral density matrix, the spectral changes in the focal plane of vector Gaussian Schell-model (GSM) beams focused by an aperture lens are studied, where the influence of correlation and polarizer on the behavior of the spectral switch is stressed. It is found that the critical position uc, spectral minimum Smin, and transition height Δ of the spectral switch depend on the auto-correlation but do not depend on the cross-correlation of GSM beams if there is no polarizer. However, in the presence of the polarizer uc, Smin and Δ depend on both auto- and cross-correlations. The polarizer affects the spectral switch of vector GSM beams, whereas it does not affect the spectral switch of scalar GSM beams. 相似文献
117.
Based on electro-optic switch effect in crystal, a novel laser ranging method is proposed. CW-laser emitted by laser transmitter propagates forward to the measured target, after being reflected by the target, and then goes back to the transmitter. Close to the transmitter, a special mono-block LiNbO3 crystal is added into the round-trip light beams. High-voltage pulses with the sharp enough changes in rising edges are loaded on the crystal. Based on electro-optic effect, double refraction and internal double reflection effect in crystal, the crystal cuts off the round-trip light beams, and reflects a light pulse cut out by the crystal to a detector aside from the original beam path. The pulse width T is the period that laser propagates forward and back between the crystal and the target. The feasibility of the new idea is proved by our experiments and a brand-new way for the laser ranging is provided. 相似文献
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All-optical shift registers are basic building modules for the development of ultra-high speed optical time division multiplexing networks. In this paper, we review the progress that has been made in this cutting-edge technology, focusing on implementations that exploit the attractive features of semiconductor optical amplifier (SOA)-based interferometric configurations. We present regenerative storage performed with an all-optical recirculating shift register with an inverter at 10 Gb/s using a SOA-assisted Sagnac switch and a second SOA to provide feedback. We demonstrate also an all-optical memory based on the SOA-assisted Ultrafast Nonlinear Interferometer capable of reading/writing 20 Gb/s packets of variable length without data inversion. These registers can find application in the development of two nontrivial complex all-optical circuits of enhanced functionality. The first is an all-optical pseudorandom binary sequence generator for which we describe an efficient design algorithm and propose ways for monitoring and verification. The second is an all-optical error counter for which we address the error detection and evaluation issues using a novel sampling technique. These circuits are key elements for the implementation of a high-speed, all-optical bit error rate tester (BERT), which has the potential to outperform its electronic equivalent and constitute a possible new product for the telecommunications industry. 相似文献