FAAS法测定新疆两种不同产地沙枣中的6种微量元素

目的建立沙枣中Ca、Mg、Cu、Zn、Fe、Mn等6种微量元素的火焰原子吸收光谱法。方法在确定的火焰原子吸收光谱法检测条件下,使用马弗炉处理样品,对新疆两种不同产地的沙枣中Ca、Mg、Cu、Zn、Fe、Mn等6种微量元素的含量进行了测定。结果各元素的回收率（／Z=5）在97．50％～104．60％之间,RSD（n=5）在1．61％～3．51％之间。结论该法简单、快速,具有很好的精密度和灵敏度,适用于沙枣中6种微量元素的测定。     

墨西哥鼠尾草与齿叶薰衣草挥发油化学成分比较

采用水蒸汽蒸馏法提取墨西哥鼠尾草和齿叶薰衣草挥发油,通过GC-MS分析其化学成分,并用峰面积归一化法计算各组分的相对含量。结果表明,墨西哥鼠尾草和齿叶薰衣草挥发油化学成分和含量有较大差别。     

Pressurized fluid extraction of essential oil from Lavandula hybrida using a modified supercritical fluid extractor and a central composite design for optimization

Essential oil components were extracted from lavandin (Lavandula hybrida) flowers using pressurized fluid extraction. A central composite design was used to optimize the effective extraction variables. The chemical composition of extracted samples was analyzed by a gas chromatograph-flame ionization detector column. For achieving 100% extraction yield, the temperature, pressure, extraction time, and the solvent flow rate were adjusted at 90.6°C, 63 bar, 30.4 min, and 0.2 mL/min, respectively. The results showed that pressurized fluid extraction is a practical technique for separation of constituents such as 1,8-cineole (8.1%), linalool (34.1%), linalyl acetate (30.5%), and camphor (7.3%) from lavandin to be applied in the food, fragrance, pharmaceutical, and natural biocides industries.     

GLC - Mass Spectroscopy of Lavandula dentata Oil

The constituents of Saudi Lavandula dentata oil were identified by two GLC-Mass spectrophotometric systems. Camphor, fechone, fenchyl alcohol and borneol were the major components of the oil. Other components were also identified.     

High-performance liquid chromatography tandem mass spectrometry for simultaneous detection of aflatoxins B1, B2, G1 and G2 in Indian medicinal herbs using QuEChERS-based extraction procedure

Since the discovery of aflatoxins (AFs) in the 1960s, much research has focused on detecting the toxins in contaminated food and feedstuffs. But the quality determination in medicinal plant matrices with respect to AFs is scare. Hence, a simple, accurate and sensitive high-performance liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) method was developed for simultaneous determination of AFs AFB₁, AFB₂, AFG₁ and AFG₂ in two Indian popular medicinal herbs i.e. senna (Cassia angustifolia) and kalmegh (Andrographis paniculata). AFs have been extracted from herb matrix using a QuEChERS (quick, easy, cheap, effective, rugged and safe)-based extraction procedure followed by applying primary secondary amine and C18 for further clean-up step and then were quantified under the multiple reaction monitoring together with positive ionisation modes. Matrix-matched calibration was used for quantification in order to reduce the matrix effect. Validation of the method was carried out in herbs by recovery experiments. Recoveries of the spiked samples were in the range of 61.9–111.5% with an inter-day and intraday relative standard deviation lower than 20.0%. Limits of detection and quantification ranged from 0.41 to 0.95 ng mL^?1 and 1.2 to 3.8 μg kg^?1, respectively. The expanded uncertainty of the method was <21% for all the toxins in both the herbs. Finally, the proposed method was successfully applied to determine AF residues in real field samples of senna and kalmegh obtained from different locations in India.     

Essential oil obtained from micropropagated lavender,its effect on HSF cells and application in cosmetic emulsion as a natural protective substance

The aim of the study was to determine the influence of the essential oils isolated from the field – grown and micropropagated in vitro narrow – leaved lavender of the ‘Munstead’ cultivar, on human skin cells, and their capability to synthesise procollagen. The amount of procollagen type I produced by fibroblast cells was determined using ELISA kit. Essential oil isolated from micropropagated lavender was further used as a protective ingredient against the development of microorganisms in O/W cosmetic emulsion. The presented results demonstrate that the use of 0.01, 0.001 and 0.0001% essential oils isolated from in vitro plants stimulate HSF cells to the production of procollagen. It was further performed that the tested essential oil used in the concentration of 0.1% in a cosmetic emulsion is characterised by preservative effect for cosmetic preparations for the period of 3 months.     

Two new dammarane triterpenoids from Kageneckia angustifolia D. Don

Two new dammarane triterpenoids, 20R,21-epoxydammar-24-ene-3,23-dione and 20R,21-epoxy-3beta-hydroxydammar-24-ene-23-one have been isolated from the aerial parts of Kageneckia angustifolia D. Don, Rosaceae, along with the previously reported triterpenoids oleanolic acid and 3beta-(beta-D-glucosyloxy)-16alpha,23alpha-epoxycucurbita-5,24-dien-11-one and the phenolic prunasin. The structures of these compounds were established by MS, 1D- and 2D-NMR experiments, and the structure of the new compounds were confirmed by X-ray diffraction analysis.     

Essential oil constituents,phenolic content and antioxidant activity of Lavandula stricta Delile growing wild in southern Iran

Lavandula stricta belongs to the Lamiaceae family and is considered as an endemic medicinal plant in southern Iran. Essential oil composition, total phenolic content and antioxidant activity from two different populations of L. stricta were studied for the first time. A GC and GC/MS analysis of essential oil isolated from the aerial part of L. stricta identified 31 constituents; the major constituents were α-pinene (58.34–63.52%), linalool (8.85–9.36%), 3-methyl butyl 2-methyl butanoate (7.45–7.70%), sabinene (2.84–3.56%), limonene (2.87–3.21%) and myrcene (2.25%). The total phenolic content of methanolic extracts was determined with the Folin–Ciocalteu reagent and the antioxidant activity of methanolic extract and essential oil were determined with the 2, 2-diphenyl-1-picryl hydrazyl free radical scavenging assay, respectively. Total phenols varied from 61.05 to 64.45 mg GAE/g dry weight, and IC₅₀ values in the radical scavenging assay ranged from 334.11 to 395.23 μg/mL in methanolic extracts and 420–475 μg/mL in essential oil.     

Chemical Composition of the Essential Oil of the New Cultivars of Lavandula angustifolia Mill. Bred in Ukraine

Lavender, otherwise known as Lavandula angustifolia Mill., is widely used in landscaping, and its oil is a valuable raw material used in many industries. Therefore, new varieties of this plant are bred. The essential oil composition obtained from fresh flowers of thirteen new Ukrainian cultivars of L. angustifolia were analysed by GC-MS, and eighty-two components were identified. Linalool and linalyl acetate were principal constituents of all of the samples, and ranged from 11.4% to 46.7% and 7.4% to 44.2%, respectively. None of the studied samples fulfilled the requirements of Ph. Eur. and ISO 3515:2002. The main reason was a high content of α-terpineol (0.5–4.5%) and/or terpinene-4-ol (1.2–18.7%). Our results are in line with multiple researchers showing that the studied lavender oils do not comply with the industry standards despite their authenticity. We also investigated the effect of the growth year on the chemical composition of five tested cultivars grown on the same plots and noticed a considerable variability between years. The obtained experimental data did not show a significant inter-year trend for the content changes of the major components. Our results allow us to deeply characterize the new cultivars and evaluate their oil for a possible use in the industry, or to designate them for future selective breeding.     

山芝麻中的新三萜化合物

山芝麻 (Helicteresangustifolia L inn.)是梧桐科 (Sterculiaceae)山芝麻属植物 ,主要分布在广东、广西、云南及长江以南各省 ,用以治疗感冒发热、麻疹、疟疾和疔疮等症 [1] .王明时等 [2 ,3]曾从该植物中分离出 3个三萜类化合物和 1个萘醌化合物 .为进一步寻找有效成分 ,我们从山芝麻中又分离到 2个新的三萜化合物 1和 2 ,用波谱方法测定了其化学结构 .1　实验部分1 .1　仪器与材料　北京泰克仪器公司的双目镜 X-6型显微熔点仪 ;EQUINOX-55(Bruker)红外光谱仪 ;Varian Unity INOVA 50 0型核磁共振仪 (TMS为内标 ) ;VG Autospec-…