A parallel-plate flow chamber consisting of two transparent electro-conductive glass plates was constructed. The two glass plates were set parallel to each other and connected to a potentiostat apparatus to regulate the strength of the electric field between the plates. A microbial cell suspension was flowed through the chamber. This system enabled the application of an electrostatic force to suspend charged particles, e.g. microbial cells, existing between the two plates. The time course of the cell attachment of Pseudomonas syringae pv. atropurpurea NIAES 1309 suspended in 10 mM phosphate buffer solution (pH 7.0) to the glass plate was investigated at various electric field strengths ranging from −4.2 to +4.1 V cm−1. The attachment rate and the maximum number of attached cells increased linearly with the increase in the strength of the positive electric field. In contrast, the rate and the number of cells decreased linearly with the decrease in the strength of the negative electric field. These linear relations gave a specific value for the strength of the electric field (−5.9 ± 0.7 V cm−1) where the electrostatic repulsion and the microbial attachment force were thought to be equal, resulting in no cell attachment. From this value, the electrostatic repulsion, i.e. the microbial attachment force, was calculated to be 5.0 × 10−11 N cell−1 for cells of average size. 相似文献
The enthalpies of crystallization of LiNO3 and LiNO3–3H2O from aqueous solutions at 25°C, measured by a calorimetric method and determined from the previously published data on the concentration dependence of the enthalpy of solution, are reported. The results are compared with the values obtained from the concentration dependences of the activity coefficients and from the temperature dependences of the solubilities. The enthalpy of solution at infinite dilution and the enthalpy of hydration are given. 相似文献
Summary High-molecular-weight sodium hyaluronate isolated from rooster combs was degraded by ultrasonication. The molecular weight of hyaluronate and its polydispersity was determined by gel-permeation chromatography. During 75-min treatment the molecular weight value decreased from 1.39×106 Da to 2.25×105 Da while the polydispersity of the molecular weight increased from 1.29 to 2.36. The reciprocal value of the square of the hyaluronate's molecular weight was linearly proportional to the time of ultrasonication. 相似文献
Summary Xylenol isomers can be resolved on most polyols (sugars or sugar alcohols) and polyethers (polyethylene glycol or polypropylene glycol) with hydrogen-bonding interaction. They are separated on vinical polyols which have more hydroxy hydrogen than tetrol, and even on vicinal triol when its hydroxy hydrogen is acidic (stronger proton donor). The stronger is the hydrogen bonding interaction between xylenols and the liquid phase, the better is the separation of 2,4- and 2,5-xylenol, and the poorer the separation of 2,4-and 2,3-xylenol. 相似文献
2-Propargyl-substituted methoxy-1,3-diketones react with alkoxymethylamines or paraform and secondary amines in the presence of CuCl at the acetylene group to afford Mannich bases.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 1293–1295, May, 1996. 相似文献
In recent years, the self-assembled growth of semiconductor nanostructures, that show quantum size effects, has been of considerable interest. Laser devices operating with self-assembled InAs quantum dots (QDs) embedded in GaAs have been demonstrated. Here, we report on the InAs/GaAs system and raise the question of how the shape of the QDs changes with the orientation of the GaAs substrate. The growth of the InAs QDs is understood in terms of the Stranski–Krastanow growth mode. For modeling the growth process, the shape and atomic structure of the QDs have to be known. This is a difficult task for such embedded entities.
In our approach, InAs is grown by molecular beam epitaxy on GaAs until self-assembled QDs are formed. At this point the growth is interrupted and atomically resolved scanning tunneling microscopy (STM) images are acquired. We used preparation parameters known from the numerous publications on InAs/GaAs. In order to learn more about the self-assemblage process we studied QD formation on different GaAs(0 0 1), (1 1 3)A, and (
)B substrates. From the atomically resolved STM images we could determine the shape of the QDs. The quantum “dots” are generally rather flat entities better characterized as “lenses”. In order to achieve this flatness, the QDs are terminated by high-index bounding facets on low-index substrates and vice versa. Our results will be summarized in comparison with the existing literature. 相似文献
We have optimized a method for the determination of intracellular glutathione by high-performance liquid chromatography, using fluorimetric detection. To minimize artifacts and provide an accurate determination of intracellular glutathione, cell extracts were prepared using extraction conditions specifically designed to inhibit autoxidation and enzymatic degradation of glutathione. The sensitivity of the method was enhanced by adjusting the dansyl chloride derivatization reaction with regard to parameters such as pH, reaction time and dansyl chloride concentration. Both oxidized and reduced forms of glutathione were quantified using the refined method in extracts of oxidatively stressed J774A.1 mouse macrophage cells and reflected an expected shift in cellular redox status. 相似文献
Palladium loaded calcium-hydroxyapatite, Pd(z)/CaHAp, and calcium-fluoroapatite, Pd(z)/CaFAp, were synthesised and characterised by TEM, XRD, IR and UV–vis–NIR spectroscopies. Introduction of palladium does not change the structure of CaHAp and CaFAp. The average size of PdO particles was found to be around 4–5 nm on Pd(1)/CaHAp but larger (6–7 nm) on Pd(1)/CaFap. The acid–base properties of the supports and of the catalysts were studied using butan-2-ol conversion. On CaHAp and CaFAp, the butenes yield (dehydration reaction) is very low either in the absence or in the presence of oxygen. The methyl ethyl ketone yield (dehydrogenation reaction) is significant only in the presence of oxygen and higher over CaFAp. Conversely, the performances of Pd(z)/CaHAp are better than those of Pd(z)/CaFAp below 180 °C. Above 180 °C, buta-2-ol combustion is favoured on Pd/CaHAp but not on Pd/CaFAp.
In methane oxidation, Pd(z)/CaHAp showed also a much larger activity than Pd(z)/CaFAp. On 2 wt% Pd loaded CaHAp, the methane oxidation reaches a conversion of almost 100% at 350 °C, which is comparable with the performance of conventional Pd/Al2O3 catalysts. The reducibility of PdO under methane–oxygen mixtures is lower on Pd(z)/CaHAp. For both reactions, the lower activity of Pd(z)/CaFAp is related to its higher acidity, resulting from the substitution of OH− by F−, and to the larger PdO particle size. 相似文献